A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity

11 páginas, 5 figuras, 1 tabla.

Detalles Bibliográficos
Autores: Smith, Aileen M., Sánchez, María José, Follows, George A., Kinston, Sarah, Donaldson, Ian J., Green, Anthony R., Göttgens, Berthold
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/41453
Acceso en línea:http://hdl.handle.net/10261/41453
Access Level:acceso abierto
Palabra clave:Basic Helix-Loop-Helix Transcription Factors
Cell differentiation
Embryonic stem cells (ESCs)
Interspersed repetitive sequence
Phylogeny
Proto-oncogene proteins
Transfection
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spelling A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activitySmith, Aileen M.Sánchez, María JoséFollows, George A.Kinston, SarahDonaldson, Ian J.Green, Anthony R.Göttgens, BertholdBasic Helix-Loop-Helix Transcription FactorsCell differentiationEmbryonic stem cells (ESCs)Interspersed repetitive sequencePhylogenyProto-oncogene proteinsTransfection11 páginas, 5 figuras, 1 tabla.Altered cis-regulation is thought to underpin much of metazoan evolution, yet the underlying mechanisms remain largely obscure. The stem cell leukemia TAL1 (also known as SCL) transcription factor is essential for the normal development of blood stem cells and we have previously shown that the Tal1 +19 enhancer directs expression to hematopoietic stem cells, hematopoietic progenitors, and to endothelium. Here we demonstrate that an adjacent region 1 kb upstream (+18 element) is in an open chromatin configuration and carries active histone marks but does not function as an enhancer in transgenic mice. Instead, it boosts activity of the +19 enhancer both in stable transfection assays and during differentiation of embryonic stem (ES) cells carrying single-copy reporter constructs targeted to the Hprt locus. The +18 element contains a mammalian interspersed repeat (MIR) which is essential for the +18 function and which was transposed to the Tal1 locus ∼160 million years ago at the time of the mammalian/marsupial branchpoint. Our data demonstrate a previously unrecognized mechanism whereby enhancer activity is modulated by a transposon exerting a “booster” function which would go undetected by conventional transgenic approaches.This work was supported by the Cambridge MIT institute, Medical Research Council (MRC), Leukemia Research Fund (LRF), Biotechnology and Biological Sciences Research Fund (BBSRC), and the Wellcome Trust.Peer reviewedCold Spring Harbor Laboratory Press201120112008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/41453reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1101/gr.077008.108info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/414532026-05-22T06:33:51Z
dc.title.none.fl_str_mv A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity
title A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity
spellingShingle A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity
Smith, Aileen M.
Basic Helix-Loop-Helix Transcription Factors
Cell differentiation
Embryonic stem cells (ESCs)
Interspersed repetitive sequence
Phylogeny
Proto-oncogene proteins
Transfection
title_short A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity
title_full A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity
title_fullStr A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity
title_full_unstemmed A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity
title_sort A novel mode of enhancer evolution: The Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity
dc.creator.none.fl_str_mv Smith, Aileen M.
Sánchez, María José
Follows, George A.
Kinston, Sarah
Donaldson, Ian J.
Green, Anthony R.
Göttgens, Berthold
author Smith, Aileen M.
author_facet Smith, Aileen M.
Sánchez, María José
Follows, George A.
Kinston, Sarah
Donaldson, Ian J.
Green, Anthony R.
Göttgens, Berthold
author_role author
author2 Sánchez, María José
Follows, George A.
Kinston, Sarah
Donaldson, Ian J.
Green, Anthony R.
Göttgens, Berthold
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Basic Helix-Loop-Helix Transcription Factors
Cell differentiation
Embryonic stem cells (ESCs)
Interspersed repetitive sequence
Phylogeny
Proto-oncogene proteins
Transfection
topic Basic Helix-Loop-Helix Transcription Factors
Cell differentiation
Embryonic stem cells (ESCs)
Interspersed repetitive sequence
Phylogeny
Proto-oncogene proteins
Transfection
description 11 páginas, 5 figuras, 1 tabla.
publishDate 2008
dc.date.none.fl_str_mv 2008
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/41453
url http://hdl.handle.net/10261/41453
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1101/gr.077008.108
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
publisher.none.fl_str_mv Cold Spring Harbor Laboratory Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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