Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity.
Objective: Sex hormone-binding globulin (SHBG) binds and transports testosterone and estradiol in plasma. The possibility that SHBG is a mixture of transporting proteins has been postulated. We analyzed in parallel the effects of obesity status on the levels and binding capacity of circulating SHBG...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/122084 |
| Acceso en línea: | https://hdl.handle.net/2445/122084 |
| Access Level: | acceso abierto |
| Palabra clave: | Hormones sexuals Obesitat mòrbida Gènere Sex hormones Morbid obesity Gender |
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Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity.Grasa Martínez, Maria del MarGulfo Cabrales, JoseCamps, NúriaAlcalá, RosaMonserrat, LauraMoreno-Navarrete, José MaríaOrtega, Francisco J.Esteve Ràfols, MontserratRemesar Betlloch, XavierFernández López, José AntonioFernández-Real Lemos, José ManuelAlemany, Marià, 1946-Hormones sexualsObesitat mòrbidaGènereSex hormonesMorbid obesityGenderObjective: Sex hormone-binding globulin (SHBG) binds and transports testosterone and estradiol in plasma. The possibility that SHBG is a mixture of transporting proteins has been postulated. We analyzed in parallel the effects of obesity status on the levels and binding capacity of circulating SHBG and their relationship with testosterone and estradiol.Design: Anthropometric measures and plasma were obtained from apparently healthy young (i.e. 35 +/- 7 years) premenopausal women (n = 32) and men (n = 30), with normal weight and obesity (BMI > 30 kg/m(2)).Methods: SHBG protein (Western blot), as well as the plasma levels of testosterone, estradiol, cortisol and insulin (ELISA) were measured. Specific binding of estradiol and testosterone to plasma SHBG was analyzed using tritiumlabeled hormones.Results: Significant differences in SHBG were observed within the obesity status and gender, with discordant patterns of change in testosterone and estradiol. In men, testosterone occupied most of the binding sites. Estrogen binding was much lower in all subjects. Lower SHBG of morbidly obese (BMI > 40 kg/m(2)) subjects affected testosterone but not estradiol. The ratio of binding sites to SHBG protein levels was constant for testosterone, but not for estradiol. The influence of gender was maximal in morbid obesity, with men showing the highest binding/SHBG ratios.Conclusions: The results reported here are compatible with SHBG being a mixture of at least two functionally different hormone-binding globulins, being affected by obesity and gender and showing different structure, affinities for testosterone and estradiol and also different immunoreactivity.Bioscientifica2018201820172018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion12 p.application/pdfhttps://hdl.handle.net/2445/122084Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1530/EJE-16-0834European Journal of Endocrinology, 2017, vol. 176, num. 4, p. 393-404https://doi.org/10.1530/EJE-16-0834(c) European Society of Endocrinology, 2017info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1220842026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity. |
| title |
Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity. |
| spellingShingle |
Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity. Grasa Martínez, Maria del Mar Hormones sexuals Obesitat mòrbida Gènere Sex hormones Morbid obesity Gender |
| title_short |
Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity. |
| title_full |
Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity. |
| title_fullStr |
Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity. |
| title_full_unstemmed |
Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity. |
| title_sort |
Modulation of SHBG binding to testosterone and estradiol by sex and morbid obesity. |
| dc.creator.none.fl_str_mv |
Grasa Martínez, Maria del Mar Gulfo Cabrales, Jose Camps, Núria Alcalá, Rosa Monserrat, Laura Moreno-Navarrete, José María Ortega, Francisco J. Esteve Ràfols, Montserrat Remesar Betlloch, Xavier Fernández López, José Antonio Fernández-Real Lemos, José Manuel Alemany, Marià, 1946- |
| author |
Grasa Martínez, Maria del Mar |
| author_facet |
Grasa Martínez, Maria del Mar Gulfo Cabrales, Jose Camps, Núria Alcalá, Rosa Monserrat, Laura Moreno-Navarrete, José María Ortega, Francisco J. Esteve Ràfols, Montserrat Remesar Betlloch, Xavier Fernández López, José Antonio Fernández-Real Lemos, José Manuel Alemany, Marià, 1946- |
| author_role |
author |
| author2 |
Gulfo Cabrales, Jose Camps, Núria Alcalá, Rosa Monserrat, Laura Moreno-Navarrete, José María Ortega, Francisco J. Esteve Ràfols, Montserrat Remesar Betlloch, Xavier Fernández López, José Antonio Fernández-Real Lemos, José Manuel Alemany, Marià, 1946- |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Hormones sexuals Obesitat mòrbida Gènere Sex hormones Morbid obesity Gender |
| topic |
Hormones sexuals Obesitat mòrbida Gènere Sex hormones Morbid obesity Gender |
| description |
Objective: Sex hormone-binding globulin (SHBG) binds and transports testosterone and estradiol in plasma. The possibility that SHBG is a mixture of transporting proteins has been postulated. We analyzed in parallel the effects of obesity status on the levels and binding capacity of circulating SHBG and their relationship with testosterone and estradiol.Design: Anthropometric measures and plasma were obtained from apparently healthy young (i.e. 35 +/- 7 years) premenopausal women (n = 32) and men (n = 30), with normal weight and obesity (BMI > 30 kg/m(2)).Methods: SHBG protein (Western blot), as well as the plasma levels of testosterone, estradiol, cortisol and insulin (ELISA) were measured. Specific binding of estradiol and testosterone to plasma SHBG was analyzed using tritiumlabeled hormones.Results: Significant differences in SHBG were observed within the obesity status and gender, with discordant patterns of change in testosterone and estradiol. In men, testosterone occupied most of the binding sites. Estrogen binding was much lower in all subjects. Lower SHBG of morbidly obese (BMI > 40 kg/m(2)) subjects affected testosterone but not estradiol. The ratio of binding sites to SHBG protein levels was constant for testosterone, but not for estradiol. The influence of gender was maximal in morbid obesity, with men showing the highest binding/SHBG ratios.Conclusions: The results reported here are compatible with SHBG being a mixture of at least two functionally different hormone-binding globulins, being affected by obesity and gender and showing different structure, affinities for testosterone and estradiol and also different immunoreactivity. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017 2018 2018 2018 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/122084 |
| url |
https://hdl.handle.net/2445/122084 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Versió postprint del document publicat a: https://doi.org/10.1530/EJE-16-0834 European Journal of Endocrinology, 2017, vol. 176, num. 4, p. 393-404 https://doi.org/10.1530/EJE-16-0834 |
| dc.rights.none.fl_str_mv |
(c) European Society of Endocrinology, 2017 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
(c) European Society of Endocrinology, 2017 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
12 p. application/pdf |
| dc.publisher.none.fl_str_mv |
Bioscientifica |
| publisher.none.fl_str_mv |
Bioscientifica |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Bioquímica i Biomedicina Molecular) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| instname_str |
Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| reponame_str |
Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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15,811543 |