Lung recovery from DNA damage induced by graphene oxide is dependent on size, dose and inflammation profile

[Background] A key aspect of any new material safety assessment is the evaluation of their in vivo genotoxicity. Graphene oxide (GO) has been studied for many promising applications, but there are remaining concerns about its safety profile, especially after inhalation. Herein we tested whether GO l...

ver descrição completa

Detalhes bibliográficos
Autores: Visani de Luna, Luis Augusto, Loret, Thomas, Fordham, Alexander, Arshad, Atta, Drummond, Matthew, Dodd, Abbie, Lozano, Neus, Kostarelos, Kostas, Bussy, Cyrill
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/287592
Acesso em linha:http://hdl.handle.net/10261/287592
Access Level:Acceso aberto
Palavra-chave:Genotoxicity
γ-H2AX
Graphene oxide
Toxicology
Inflammation
Lungs
Descrição
Resumo:[Background] A key aspect of any new material safety assessment is the evaluation of their in vivo genotoxicity. Graphene oxide (GO) has been studied for many promising applications, but there are remaining concerns about its safety profile, especially after inhalation. Herein we tested whether GO lateral dimension, comparing micrometric (LGO) and nanometric (USGO) GO sheets, has a role in the formation of DNA double strand breaks in mouse lungs. We used spatial resolution and differential cell type analysis to measure DNA damages in both epithelial and immune cells, after either single or repeated exposure.