Improved biostimulant production through solid-state fermentation of green waste using low-temperature strategy in tray bioreactors
This study investigates solid-state fermentation (SSF) of Trichoderma harzianum on green waste (GW) to produce the biostimulant indole-3-acetic acid (IAA) and conidial spores. Initial experiments without temperature control achieved high yields (201.3 µg g-1 dry matter (DM) of IAA and 1.1 × 109 spor...
| Authors: | , , , |
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| Format: | article |
| Publication Date: | 2026 |
| Country: | España |
| Institution: | Universitat Autònoma de Barcelona |
| Repository: | Dipòsit Digital de Documents de la UAB |
| Language: | English |
| OAI Identifier: | oai:ddd.uab.cat:326251 |
| Online Access: | https://ddd.uab.cat/record/326251 https://dx.doi.org/urn:doi:10.1016/j.biortech.2026.133931 |
| Access Level: | Open access |
| Keyword: | Biopesticide Indole-3-acetic acid Principal component analysis Trichoderma harzianum |
| Summary: | This study investigates solid-state fermentation (SSF) of Trichoderma harzianum on green waste (GW) to produce the biostimulant indole-3-acetic acid (IAA) and conidial spores. Initial experiments without temperature control achieved high yields (201.3 µg g-1 dry matter (DM) of IAA and 1.1 × 109 spores g-1 DM) exceeding reported literature values. However, variability was observed in the production of IAA. A principal component analysis revealed that temperature, especially in the later stages of fermentation, was intricately linked to the final IAA levels. Based on these findings, a new process strategy was developed, applying a low-temperature pattern to prevent IAA decrease and enhance its production. Experimental validation confirmed improved reproducibility and efficiency, yielding 199.9 µg g-1 DM of IAA and 1.56 × 108 spores g-1 DM. This work suggests a novel and easily scalable SSF approach that simultaneously produces biostimulants and biopesticides while valorizing green waste, contributing to sustainable practices within a circular bioeconomy. |
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