Improved biostimulant production through solid-state fermentation of green waste using low-temperature strategy in tray bioreactors

This study investigates solid-state fermentation (SSF) of Trichoderma harzianum on green waste (GW) to produce the biostimulant indole-3-acetic acid (IAA) and conidial spores. Initial experiments without temperature control achieved high yields (201.3 µg g-1 dry matter (DM) of IAA and 1.1 × 109 spor...

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Detalles Bibliográficos
Autores: Ghoreishi, Golafarin|||0000-0002-1682-9310, Font, Xavier|||0000-0003-4981-7436, Sánchez, Antoni|||0000-0003-4254-8528, Barrena, Raquel|||0000-0002-6077-7765
Tipo de recurso: artículo
Fecha de publicación:2026
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:326251
Acceso en línea:https://ddd.uab.cat/record/326251
https://dx.doi.org/urn:doi:10.1016/j.biortech.2026.133931
Access Level:acceso abierto
Palabra clave:Biopesticide
Indole-3-acetic acid
Principal component analysis
Trichoderma harzianum
Descripción
Sumario:This study investigates solid-state fermentation (SSF) of Trichoderma harzianum on green waste (GW) to produce the biostimulant indole-3-acetic acid (IAA) and conidial spores. Initial experiments without temperature control achieved high yields (201.3 µg g-1 dry matter (DM) of IAA and 1.1 × 109 spores g-1 DM) exceeding reported literature values. However, variability was observed in the production of IAA. A principal component analysis revealed that temperature, especially in the later stages of fermentation, was intricately linked to the final IAA levels. Based on these findings, a new process strategy was developed, applying a low-temperature pattern to prevent IAA decrease and enhance its production. Experimental validation confirmed improved reproducibility and efficiency, yielding 199.9 µg g-1 DM of IAA and 1.56 × 108 spores g-1 DM. This work suggests a novel and easily scalable SSF approach that simultaneously produces biostimulants and biopesticides while valorizing green waste, contributing to sustainable practices within a circular bioeconomy.