Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3D

Synthetic dendrimer peptides are a promising strategy to develop new FMD vaccines. A dendrimer peptide, termed B2T-3A, which harbors two copies of the major FMDV antigenic B-cell site [VP1 (140-158)], covalently linked to a heterotypic T-cell from the non-structural protein 3A [3A (21-35)], has been...

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Autores: Cañas Arranz, Rodrigo, de León, Patricia, Forner, Mar, 1980-, Defaus, Sira, Bustos, María J., Torres, Elisa, Andreu Martínez, David, Blanco, Esther, Sobrino, Francisco
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2020
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/45410
Acesso em linha:http://hdl.handle.net/10230/45410
http://dx.doi.org/10.3389/fvets.2020.00498
Access Level:Acceso aberto
Palavra-chave:FMDV
T-cell epitopes
dendrimer peptides
Swine
Vaccines
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spelling Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3DCañas Arranz, Rodrigode León, PatriciaForner, Mar, 1980-Defaus, SiraBustos, María J.Torres, ElisaAndreu Martínez, DavidBlanco, EstherSobrino, FranciscoFMDVT-cell epitopesdendrimer peptidesSwineVaccinesSynthetic dendrimer peptides are a promising strategy to develop new FMD vaccines. A dendrimer peptide, termed B2T-3A, which harbors two copies of the major FMDV antigenic B-cell site [VP1 (140-158)], covalently linked to a heterotypic T-cell from the non-structural protein 3A [3A (21-35)], has been shown to protect pigs against viral challenge. Interestingly, the modular design of this dendrimer peptide allows modifications aimed at improving its immunogenicity, such as the replacement of the T-cell epitope moiety. Here, we report that a dendrimer peptide, B2T-3D, harboring a T-cell epitope from FMDV 3D protein [3D (56-70)], when inoculated in pigs, elicited consistent levels of neutralizing antibodies and high frequencies of IFN-γ-producing cells upon in vitro recall with the homologous dendrimers, both responses being similar to those evoked by B2T-3A. Lymphocytes from B2T-3A-immunized pigs were in vitro-stimulated by T-3A peptide and to a lesser extent by B-peptide, while those from B2T-3D- immunized animals preferentially recognized the T-3D peptide, suggesting that this epitope is a potent inducer of IFN-γ producing-cells. These results extend the repertoire of T-cell epitopes efficiently recognized by swine lymphocytes and open the possibility of using T-3D to enhance the immunogenicity and the protection conferred by B2T-dendrimers.Frontiers202020202020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/45410http://dx.doi.org/10.3389/fvets.2020.00498reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésFront Vet Sci. 2020; 7:498© 2020 Cañas-Arranz, de León, Forner, Defaus, Bustos, Torres, Andreu, Blanco and Sobrino. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/454102026-05-29T05:05:01Z
dc.title.none.fl_str_mv Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3D
title Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3D
spellingShingle Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3D
Cañas Arranz, Rodrigo
FMDV
T-cell epitopes
dendrimer peptides
Swine
Vaccines
title_short Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3D
title_full Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3D
title_fullStr Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3D
title_full_unstemmed Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3D
title_sort Immunogenicity of a dendrimer B 2 T peptide harboring a T-cell epitope from FMDV non-structural protein 3D
dc.creator.none.fl_str_mv Cañas Arranz, Rodrigo
de León, Patricia
Forner, Mar, 1980-
Defaus, Sira
Bustos, María J.
Torres, Elisa
Andreu Martínez, David
Blanco, Esther
Sobrino, Francisco
author Cañas Arranz, Rodrigo
author_facet Cañas Arranz, Rodrigo
de León, Patricia
Forner, Mar, 1980-
Defaus, Sira
Bustos, María J.
Torres, Elisa
Andreu Martínez, David
Blanco, Esther
Sobrino, Francisco
author_role author
author2 de León, Patricia
Forner, Mar, 1980-
Defaus, Sira
Bustos, María J.
Torres, Elisa
Andreu Martínez, David
Blanco, Esther
Sobrino, Francisco
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FMDV
T-cell epitopes
dendrimer peptides
Swine
Vaccines
topic FMDV
T-cell epitopes
dendrimer peptides
Swine
Vaccines
description Synthetic dendrimer peptides are a promising strategy to develop new FMD vaccines. A dendrimer peptide, termed B2T-3A, which harbors two copies of the major FMDV antigenic B-cell site [VP1 (140-158)], covalently linked to a heterotypic T-cell from the non-structural protein 3A [3A (21-35)], has been shown to protect pigs against viral challenge. Interestingly, the modular design of this dendrimer peptide allows modifications aimed at improving its immunogenicity, such as the replacement of the T-cell epitope moiety. Here, we report that a dendrimer peptide, B2T-3D, harboring a T-cell epitope from FMDV 3D protein [3D (56-70)], when inoculated in pigs, elicited consistent levels of neutralizing antibodies and high frequencies of IFN-γ-producing cells upon in vitro recall with the homologous dendrimers, both responses being similar to those evoked by B2T-3A. Lymphocytes from B2T-3A-immunized pigs were in vitro-stimulated by T-3A peptide and to a lesser extent by B-peptide, while those from B2T-3D- immunized animals preferentially recognized the T-3D peptide, suggesting that this epitope is a potent inducer of IFN-γ producing-cells. These results extend the repertoire of T-cell epitopes efficiently recognized by swine lymphocytes and open the possibility of using T-3D to enhance the immunogenicity and the protection conferred by B2T-dendrimers.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/45410
http://dx.doi.org/10.3389/fvets.2020.00498
url http://hdl.handle.net/10230/45410
http://dx.doi.org/10.3389/fvets.2020.00498
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Front Vet Sci. 2020; 7:498
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers
publisher.none.fl_str_mv Frontiers
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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