Immunogenicity of foot-and-mouth disease virus dendrimer peptides: need for a T-cell epitope and ability to elicit heterotypic responses

An approach based on a dendrimer display of B- and T-cell epitopes relevant for antibody induction has been shown to be effective as a foot-and-mouth disease (FMD) vaccine. B2T dendrimers combining two copies of the major FMD virus (FMDV) type O B-cell epitope (capsid proteinVP1 (140-158)) covalentl...

Descripción completa

Detalles Bibliográficos
Autores: Cañas Arranz, Rodrigo, de León, Patricia, Defaus, Sira, Torres, Elisa, Forner, Mar, 1980-, Bustos, María J., Andreu Martínez, David, Blanco, Esther, Sobrino, Francisco
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/48664
Acceso en línea:http://hdl.handle.net/10230/48664
http://dx.doi.org/10.3390/molecules26164714
Access Level:acceso abierto
Palabra clave:FMDV
Dendrimer
Peptide vaccine
Descripción
Sumario:An approach based on a dendrimer display of B- and T-cell epitopes relevant for antibody induction has been shown to be effective as a foot-and-mouth disease (FMD) vaccine. B2T dendrimers combining two copies of the major FMD virus (FMDV) type O B-cell epitope (capsid proteinVP1 (140-158)) covalently linked to a heterotypic T-cell epitope from non-structural protein 3A (21-35), henceforth B2T-3A, has previously been shown to elicit high neutralizing antibody (nAb) titers and IFN-γ-producing cells in both mice and pigs. Here, we provide evidence that the B- and T-cell epitopes need to be tethered to a single molecular platform for successful T-cell help, leading to efficient nAb induction in mice. In addition, mice immunized with a non-covalent mixture of B2T-3A dendrimers containing the B-cell epitopes of FMDV types O and C induced similarly high nAb levels against both serotypes, opening the way for a multivalent vaccine platform against a variety of serologically different FMDVs. These findings are relevant for the design of vaccine strategies based on B- and T-cell epitope combinations.

Similares en LA Referencia