Conservation of Phenotypes in the Roman High- and Low-Avoidance Rat Strains After Embryo Transfer

The Roman high- (RHA-I) and low-avoidance (RLA-I) rat strains are bi-directionally bred for their good versus non-acquisition of two-way active avoidance, respectively. They have recently been re-derived through embryo transfer (ET) to Sprague-Dawley females to generate specific pathogen free (SPF)...

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Detalles Bibliográficos
Autores: Río-Álamos, Cristóbal|||0000-0002-5676-4494, Gerbolés, Cristina|||0000-0002-9587-1014, Tapias-Espinosa, Carles|||0000-0003-3110-0138, Sampedro-Viana, Daniel|||0000-0003-3922-4039, Oliveras, Ignasi|||0000-0002-3082-0355, Sánchez González, Ana, Cañete, Toni|||0000-0002-8950-8120, Blázquez Romero, Glòria, López, María del Mar, Baldellou, Carlos, Otaegui Goya, Pedro José, Tobeña, Adolf|||0000-0001-6137-0660, Fernández-Teruel, Alberto|||0000-0001-5993-7058
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:285949
Acceso en línea:https://ddd.uab.cat/record/285949
https://dx.doi.org/urn:doi:10.1007/s10519-017-9854-2
Access Level:acceso abierto
Palabra clave:Behavioural phenotyping
Embryo transfer
Roman rat strains
Stress-induced corticosterone
Two-way active avoidance
Descripción
Sumario:The Roman high- (RHA-I) and low-avoidance (RLA-I) rat strains are bi-directionally bred for their good versus non-acquisition of two-way active avoidance, respectively. They have recently been re-derived through embryo transfer (ET) to Sprague-Dawley females to generate specific pathogen free (SPF) RHA-I/RLA-I rats. Offspring were phenotyped at generations 1 (G1, born from Sprague-Dawley females), 3 and 5 (G3 and G5, born from RHA-I and RLA-I from G2-G4, respectively), and compared with generation 60 from our non-SPF colony. Phenotyping included two-way avoidance acquisition, context-conditioned fear, open-field behaviour, novelty-seeking, baseline startle, pre-pulse inhibition (PPI) and stress-induced increase in plasma corticosterone concentration. Post-ET between-strain differences in avoidance acquisition, context-conditioned freezing and novelty-induced self-grooming are conserved. Other behavioural traits (i.e. hole-board head-dipping, novel object exploration, open-field activity, startle, PPI) differentiate the strains at G3-G5 but not at G1, suggesting that the pre-/post-natal environment may have influenced these co-segregated traits at G1, though further selection pressure along the subsequent generations (G1-G5) rescues the typical strain-related differences.

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