Liquid chromatography-mass spectrometry

Since the development of atmospheric pressure ionisation (API), especially the electrospray ionisation (ESI) interface, liquid chromatography-mass spectrometry (LC-MS) has become a routine analytical tool. The strength of LC-MS lies in its ability to selectively determine trace analytes in very comp...

ver descrição completa

Detalhes bibliográficos
Autores: Caixach, Josep, Flores, Cintia, Spoof, Lisa, Meriluoto, Jussi, Schmidt, Wido, Mazur-Marzec, Hanna, Hiskia, Anastasia, Kaloudis, Triantafyllos, Furey, Ambrose
Formato: otro
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/414991
Acesso em linha:http://hdl.handle.net/10261/414991
https://api.elsevier.com/content/abstract/scopus_id/85041524466
Access Level:acceso abierto
Palavra-chave:LC-MS
Liquid Chromatography–Mass Spectrometry
http://metadata.un.org/sdg/9
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
id ES_8bc0eacf3ec40270bbd5f8d92a6e25ea
oai_identifier_str oai:digital.csic.es:10261/414991
network_acronym_str ES
network_name_str España
repository_id_str
spelling Liquid chromatography-mass spectrometryCaixach, JosepFlores, CintiaSpoof, LisaMeriluoto, JussiSchmidt, WidoMazur-Marzec, HannaHiskia, AnastasiaKaloudis, TriantafyllosFurey, AmbroseLC-MSLiquid Chromatography–Mass Spectrometryhttp://metadata.un.org/sdg/9http://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesBuild resilient infrastructure, promote inclusive and sustainable industrialization and foster innovationSince the development of atmospheric pressure ionisation (API), especially the electrospray ionisation (ESI) interface, liquid chromatography-mass spectrometry (LC-MS) has become a routine analytical tool. The strength of LC-MS lies in its ability to selectively determine trace analytes in very complex sample matrices. The fast scanning speeds of modern MS instruments and the wide selection of MS technologies available, including instruments with the capability to perform tandem MS (MS/MS) and even multigenerational MS (MSn), along with instruments that can provide high mass accuracy, make this technology ideal for identifying multiple target compounds in a single analytical run. These multiple attributes and abilities have made LC-MS a very valuable tool in the characterisation of naturally occurring bioactive molecules and their analogues in a wide range of sample types. However, with use comes knowledge and nowadays LC-MS users realise that the technique is not the panacea they once believed it to be. Matrix-dependent ion suppression can be a significant problem with real samples impinging negatively on critical experimental parameters including LOD, LOQ, linearity, etc. Thankfully researchers have found ways to identify, quantify and circumnavigate this problem; these approaches now form part of the routine protocol employed for the validation of LC-MS-based methods. The objective of this review is to provide an up-to-date overview of the current techniques in LC-MS and its application for cyanobacterial toxin characterisation.Peer reviewedWiley-VCHConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262017info:eu-repo/semantics/otherhttp://purl.org/coar/resource_type/c_3248Postprintinfo:eu-repo/semantics/acceptedVersioninfo:eu-repo/semantics/bookParthttp://hdl.handle.net/10261/414991https://api.elsevier.com/content/abstract/scopus_id/85041524466reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésHandbook of Cyanobacterial Monitoring and Cyanotoxin Analysishttps://doi.org/10.1002/9781119068761.ch23Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4149912026-05-22T06:33:51Z
dc.title.none.fl_str_mv Liquid chromatography-mass spectrometry
title Liquid chromatography-mass spectrometry
spellingShingle Liquid chromatography-mass spectrometry
Caixach, Josep
LC-MS
Liquid Chromatography–Mass Spectrometry
http://metadata.un.org/sdg/9
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
title_short Liquid chromatography-mass spectrometry
title_full Liquid chromatography-mass spectrometry
title_fullStr Liquid chromatography-mass spectrometry
title_full_unstemmed Liquid chromatography-mass spectrometry
title_sort Liquid chromatography-mass spectrometry
dc.creator.none.fl_str_mv Caixach, Josep
Flores, Cintia
Spoof, Lisa
Meriluoto, Jussi
Schmidt, Wido
Mazur-Marzec, Hanna
Hiskia, Anastasia
Kaloudis, Triantafyllos
Furey, Ambrose
author Caixach, Josep
author_facet Caixach, Josep
Flores, Cintia
Spoof, Lisa
Meriluoto, Jussi
Schmidt, Wido
Mazur-Marzec, Hanna
Hiskia, Anastasia
Kaloudis, Triantafyllos
Furey, Ambrose
author_role author
author2 Flores, Cintia
Spoof, Lisa
Meriluoto, Jussi
Schmidt, Wido
Mazur-Marzec, Hanna
Hiskia, Anastasia
Kaloudis, Triantafyllos
Furey, Ambrose
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv LC-MS
Liquid Chromatography–Mass Spectrometry
http://metadata.un.org/sdg/9
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
topic LC-MS
Liquid Chromatography–Mass Spectrometry
http://metadata.un.org/sdg/9
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
Build resilient infrastructure, promote inclusive and sustainable industrialization and foster innovation
description Since the development of atmospheric pressure ionisation (API), especially the electrospray ionisation (ESI) interface, liquid chromatography-mass spectrometry (LC-MS) has become a routine analytical tool. The strength of LC-MS lies in its ability to selectively determine trace analytes in very complex sample matrices. The fast scanning speeds of modern MS instruments and the wide selection of MS technologies available, including instruments with the capability to perform tandem MS (MS/MS) and even multigenerational MS (MSn), along with instruments that can provide high mass accuracy, make this technology ideal for identifying multiple target compounds in a single analytical run. These multiple attributes and abilities have made LC-MS a very valuable tool in the characterisation of naturally occurring bioactive molecules and their analogues in a wide range of sample types. However, with use comes knowledge and nowadays LC-MS users realise that the technique is not the panacea they once believed it to be. Matrix-dependent ion suppression can be a significant problem with real samples impinging negatively on critical experimental parameters including LOD, LOQ, linearity, etc. Thankfully researchers have found ways to identify, quantify and circumnavigate this problem; these approaches now form part of the routine protocol employed for the validation of LC-MS-based methods. The objective of this review is to provide an up-to-date overview of the current techniques in LC-MS and its application for cyanobacterial toxin characterisation.
publishDate 2017
dc.date.none.fl_str_mv 2017
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/other
http://purl.org/coar/resource_type/c_3248
Postprint
info:eu-repo/semantics/acceptedVersion
dc.type.openaire.fl_str_mv info:eu-repo/semantics/bookPart
format other
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/414991
https://api.elsevier.com/content/abstract/scopus_id/85041524466
url http://hdl.handle.net/10261/414991
https://api.elsevier.com/content/abstract/scopus_id/85041524466
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Handbook of Cyanobacterial Monitoring and Cyanotoxin Analysis
https://doi.org/10.1002/9781119068761.ch23
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412848936943616
score 15,812429

Registos relacionados