Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas cultures

1,2-dichloropropane (1,2-DCP) and 1,2,3-trichloropropane (1,2,3-TCP) are hazardous chemicals frequently detected in groundwater near agricultural zones due to their historical use in chlorinated fumigant formulations. In this study, we show that the organohalide-respiring bacterium <em style=&quo...

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Autores: Trueba-Santiso, Alba, Torrentó, Clara, Soder-Walz, Jesica M., Fernández-Verdejo, David, Rosell, Mònica, Marco-Urrea, Ernest
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2024
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/215727
Acceso en línea:https://hdl.handle.net/2445/215727
Access Level:acceso abierto
Palabra clave:Hidrologia d'aigües subterrànies
Contaminació de l'aigua
Isòtops
Groundwater hydrology
Water pollution
Isotopes
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spelling Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas culturesTrueba-Santiso, AlbaTorrentó, ClaraSoder-Walz, Jesica M.Fernández-Verdejo, DavidRosell, MònicaMarco-Urrea, ErnestHidrologia d'aigües subterràniesContaminació de l'aiguaIsòtopsGroundwater hydrologyWater pollutionIsotopes1,2-dichloropropane (1,2-DCP) and 1,2,3-trichloropropane (1,2,3-TCP) are hazardous chemicals frequently detected in groundwater near agricultural zones due to their historical use in chlorinated fumigant formulations. In this study, we show that the organohalide-respiring bacterium <em style="color:black">Dehalogenimonas alkenigignens</em><span style="color:black"> strain BRE15M can grow during the dihaloelimination of </span>1,2-DCP and 1,2,3-TCP to propene and allyl chloride, respectively. Our work also provides the first application of dual isotope approach to investigate the anaerobic reductive dechlorination of 1,2-DCP and 1,2,3-TCP. Stable carbon and chlorine isotope fractionation values for 1,2-DCP (Ɛ<sub>C </sub>= -13.6 ± 1.4 ‰ and Ɛ<sub>Cl</sub>= -27.4 ± 5.2 ‰) and 1,2,3-TCP (Ɛ<sub>C </sub>= -3.8 ± 0.6 ‰ and Ɛ<sub>Cl</sub>= -0.8 ± 0.5 ‰) were obtained resulting in distinct dual isotope slopes (Λ<sub>12DCP</sub>= 0.5 ± 0.1, Λ<sub>123TCP</sub>= 4 ± 2). However direct comparison of Λ<sub>C-Cl</sub> among different substrates is not possible and investigation of the C and Cl apparent kinetic isotope effects lead to the hypothesis that <em>concerted </em>dichloroelimination mechanism is more likely for both compounds. In fact, whole cell activity assays using cells suspensions of the <em>Dehalogenimonas</em>-containing culture grown with 1,2-DCP and methyl viologen as electron donor suggest that the same set of reductive dehalogenases was involved in the transformation of 1,2-DCP and 1,2,3-TCP. This study opens the door to the application of isotope techniques for evaluating biodegradation of 1,2-DCP and 1,2,3-TCP, which often co-occur in groundwaters near agricultural fields.Elsevier Ltd2024202420242024info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion11 p.application/pdfhttps://hdl.handle.net/2445/215727Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.chemosphere.2024.142170Chemosphere, 2024, vol. 358https://doi.org/10.1016/j.chemosphere.2024.142170cc-by-nc-nd (c) The Author(s), 2024http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/2157272026-05-29T05:05:01Z
dc.title.none.fl_str_mv Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas cultures
title Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas cultures
spellingShingle Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas cultures
Trueba-Santiso, Alba
Hidrologia d'aigües subterrànies
Contaminació de l'aigua
Isòtops
Groundwater hydrology
Water pollution
Isotopes
title_short Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas cultures
title_full Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas cultures
title_fullStr Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas cultures
title_full_unstemmed Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas cultures
title_sort Dual C–Cl isotope fractionation offers potential to assess biodegradation of 1,2-dichloropropane and 1,2,3-trichloropropane by Dehalogenimonas cultures
dc.creator.none.fl_str_mv Trueba-Santiso, Alba
Torrentó, Clara
Soder-Walz, Jesica M.
Fernández-Verdejo, David
Rosell, Mònica
Marco-Urrea, Ernest
author Trueba-Santiso, Alba
author_facet Trueba-Santiso, Alba
Torrentó, Clara
Soder-Walz, Jesica M.
Fernández-Verdejo, David
Rosell, Mònica
Marco-Urrea, Ernest
author_role author
author2 Torrentó, Clara
Soder-Walz, Jesica M.
Fernández-Verdejo, David
Rosell, Mònica
Marco-Urrea, Ernest
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Hidrologia d'aigües subterrànies
Contaminació de l'aigua
Isòtops
Groundwater hydrology
Water pollution
Isotopes
topic Hidrologia d'aigües subterrànies
Contaminació de l'aigua
Isòtops
Groundwater hydrology
Water pollution
Isotopes
description 1,2-dichloropropane (1,2-DCP) and 1,2,3-trichloropropane (1,2,3-TCP) are hazardous chemicals frequently detected in groundwater near agricultural zones due to their historical use in chlorinated fumigant formulations. In this study, we show that the organohalide-respiring bacterium <em style="color:black">Dehalogenimonas alkenigignens</em><span style="color:black"> strain BRE15M can grow during the dihaloelimination of </span>1,2-DCP and 1,2,3-TCP to propene and allyl chloride, respectively. Our work also provides the first application of dual isotope approach to investigate the anaerobic reductive dechlorination of 1,2-DCP and 1,2,3-TCP. Stable carbon and chlorine isotope fractionation values for 1,2-DCP (Ɛ<sub>C </sub>= -13.6 ± 1.4 ‰ and Ɛ<sub>Cl</sub>= -27.4 ± 5.2 ‰) and 1,2,3-TCP (Ɛ<sub>C </sub>= -3.8 ± 0.6 ‰ and Ɛ<sub>Cl</sub>= -0.8 ± 0.5 ‰) were obtained resulting in distinct dual isotope slopes (Λ<sub>12DCP</sub>= 0.5 ± 0.1, Λ<sub>123TCP</sub>= 4 ± 2). However direct comparison of Λ<sub>C-Cl</sub> among different substrates is not possible and investigation of the C and Cl apparent kinetic isotope effects lead to the hypothesis that <em>concerted </em>dichloroelimination mechanism is more likely for both compounds. In fact, whole cell activity assays using cells suspensions of the <em>Dehalogenimonas</em>-containing culture grown with 1,2-DCP and methyl viologen as electron donor suggest that the same set of reductive dehalogenases was involved in the transformation of 1,2-DCP and 1,2,3-TCP. This study opens the door to the application of isotope techniques for evaluating biodegradation of 1,2-DCP and 1,2,3-TCP, which often co-occur in groundwaters near agricultural fields.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/215727
url https://hdl.handle.net/2445/215727
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.chemosphere.2024.142170
Chemosphere, 2024, vol. 358
https://doi.org/10.1016/j.chemosphere.2024.142170
dc.rights.none.fl_str_mv cc-by-nc-nd (c) The Author(s), 2024
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) The Author(s), 2024
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ltd
publisher.none.fl_str_mv Elsevier Ltd
dc.source.none.fl_str_mv Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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