Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.

Circulating extracellular vesicles (EVs) have a great impact on human health as biomarkers and messengers in intercellular signalling. We aimed to determine how the miRNA profile of circulating EVs during an acute coronary event interferes with the vasculogenic potential of endothelial cells (EC). E...

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Autores: Bobi, Joaquin, Jimenez-Trinidad, Francisco-Rafael, Ortega-Paz, Luis, Cortes-Serra, Nuria, Lazaro, Iolanda, Rodriguez, Juan-Jose, Fernandez-Becerra, Carmen, García-Álvarez, Ana, Sabate, Manel, Brugaletta, Salvatore, Dantas, Ana-Paula
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/27046
Acceso en línea:https://hdl.handle.net/20.500.12105/27046
Access Level:acceso abierto
Palabra clave:acute coronary syndrome
angiogenesis
extracellular vesicles
miRNA profile
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spelling Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.Bobi, JoaquinJimenez-Trinidad, Francisco-RafaelOrtega-Paz, LuisCortes-Serra, NuriaLazaro, IolandaRodriguez, Juan-JoseFernandez-Becerra, CarmenGarcía-Álvarez, AnaSabate, ManelBrugaletta, SalvatoreDantas, Ana-Paulaacute coronary syndromeangiogenesisextracellular vesiclesmiRNA profileCirculating extracellular vesicles (EVs) have a great impact on human health as biomarkers and messengers in intercellular signalling. We aimed to determine how the miRNA profile of circulating EVs during an acute coronary event interferes with the vasculogenic potential of endothelial cells (EC). EVs were purified from the plasma of patients in the acute phase of non-ST segment elevation myocardial infarction (NSTEMI, n = 33) and from healthy donors (n = 19) used as a control group. Human ECs were treated with EV suspension (5 × 10 particles/cm) and tested for their vasculogenic potential and mRNA expression. The EV miRNA profile was determined by miRNA array. EV levels were markedly increased in the plasma of NSTEMI (2.3 × 10 ± 1.5 × 10 particles/mL) versus control (1.2 × 10 ± 1.1 × 10 particles/mL; p = .02). Treatment of ECs with control EVs increased migration, tube formation, and shaped more branched vessel-like structures in comparison to Sham-treated ECs. Nevertheless, EVs from NSTEMI lacked their vasculogenic potential. Network analysis of EV miRNA and EC mRNA expression revealed a correlation of increased miR-199a-3p and miR-125a-5p expression with a decrease in components involved in EC sprout and stabilization. Combined therapy with miR-199a-3p and miR-125a-5p decreased EC vasculogenic potential. Moreover, anti-miRNA therapy with a combination of anti-miR-125a-5p and anti-miR-199a-3p restored the vasculogenic potential impaired by NSTEMI EVs. Circulating EVs play an important role in the control of angiogenesis. However, in the acute phase of NSTEMI, intercellular communication via EV is modified and loses its ability to generate new blood vessels. The loss of angiogenic capacity of EVs during NSTEMI may be an important player in the disease progression and outcomes.WILEY20252025-12-1620252025-07-0120252025-07-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12105/27046reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/270462026-06-12T12:43:37Z
dc.title.none.fl_str_mv Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.
title Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.
spellingShingle Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.
Bobi, Joaquin
acute coronary syndrome
angiogenesis
extracellular vesicles
miRNA profile
title_short Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.
title_full Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.
title_fullStr Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.
title_full_unstemmed Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.
title_sort Antiangiogenic effect of circulating extracellular vesicles in acute coronary syndrome: Role of miR-199a-3p and miR-125a-5p.
dc.creator.none.fl_str_mv Bobi, Joaquin
Jimenez-Trinidad, Francisco-Rafael
Ortega-Paz, Luis
Cortes-Serra, Nuria
Lazaro, Iolanda
Rodriguez, Juan-Jose
Fernandez-Becerra, Carmen
García-Álvarez, Ana
Sabate, Manel
Brugaletta, Salvatore
Dantas, Ana-Paula
author Bobi, Joaquin
author_facet Bobi, Joaquin
Jimenez-Trinidad, Francisco-Rafael
Ortega-Paz, Luis
Cortes-Serra, Nuria
Lazaro, Iolanda
Rodriguez, Juan-Jose
Fernandez-Becerra, Carmen
García-Álvarez, Ana
Sabate, Manel
Brugaletta, Salvatore
Dantas, Ana-Paula
author_role author
author2 Jimenez-Trinidad, Francisco-Rafael
Ortega-Paz, Luis
Cortes-Serra, Nuria
Lazaro, Iolanda
Rodriguez, Juan-Jose
Fernandez-Becerra, Carmen
García-Álvarez, Ana
Sabate, Manel
Brugaletta, Salvatore
Dantas, Ana-Paula
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv acute coronary syndrome
angiogenesis
extracellular vesicles
miRNA profile
topic acute coronary syndrome
angiogenesis
extracellular vesicles
miRNA profile
description Circulating extracellular vesicles (EVs) have a great impact on human health as biomarkers and messengers in intercellular signalling. We aimed to determine how the miRNA profile of circulating EVs during an acute coronary event interferes with the vasculogenic potential of endothelial cells (EC). EVs were purified from the plasma of patients in the acute phase of non-ST segment elevation myocardial infarction (NSTEMI, n = 33) and from healthy donors (n = 19) used as a control group. Human ECs were treated with EV suspension (5 × 10 particles/cm) and tested for their vasculogenic potential and mRNA expression. The EV miRNA profile was determined by miRNA array. EV levels were markedly increased in the plasma of NSTEMI (2.3 × 10 ± 1.5 × 10 particles/mL) versus control (1.2 × 10 ± 1.1 × 10 particles/mL; p = .02). Treatment of ECs with control EVs increased migration, tube formation, and shaped more branched vessel-like structures in comparison to Sham-treated ECs. Nevertheless, EVs from NSTEMI lacked their vasculogenic potential. Network analysis of EV miRNA and EC mRNA expression revealed a correlation of increased miR-199a-3p and miR-125a-5p expression with a decrease in components involved in EC sprout and stabilization. Combined therapy with miR-199a-3p and miR-125a-5p decreased EC vasculogenic potential. Moreover, anti-miRNA therapy with a combination of anti-miR-125a-5p and anti-miR-199a-3p restored the vasculogenic potential impaired by NSTEMI EVs. Circulating EVs play an important role in the control of angiogenesis. However, in the acute phase of NSTEMI, intercellular communication via EV is modified and loses its ability to generate new blood vessels. The loss of angiogenic capacity of EVs during NSTEMI may be an important player in the disease progression and outcomes.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-12-16
2025
2025-07-01
2025
2025-07-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/27046
url https://hdl.handle.net/20.500.12105/27046
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv WILEY
publisher.none.fl_str_mv WILEY
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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