Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain

Objectives: To carry out the characterization of the genes encoding extended-spectrum β-lactamases (ESBLs) and their genetic environments in four expanded-spectrum cephalosporin-resistant Salmonella enterica isolates (serovars: two Virchow, one Enteritidis, one Rissen) recovered during the monitorin...

Descripción completa

Detalles Bibliográficos
Autores: Riaño, I. [0000-0001-8410-2706], Moreno, M.A. [0000-0002-5357-3684], Teshager, T., Sáenz, Y. [0000-0002-2457-4258], Domínguez, L. [0000-0001-8843-0010], Torres, C. [0000-0003-3709-1690]
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2006
País:España
Institución:Universidad de La Rioja (UR)
Repositorio:RIUR. Repositorio Institucional de la Universidad de La Rioja
OAI Identifier:oai:portal.dialnet.es:doc/5bbc68f9b750603269e812b9
Acceso en línea:https://investigacion.unirioja.es/documentos/5bbc68f9b750603269e812b9
Access Level:acceso abierto
Palabra clave:CTX-M-9
ESBLs
Integrons
S. enterica
SHV-12
id ES_8a05ea8fefd49fd1af8e3dbfc4b53c01
oai_identifier_str oai:portal.dialnet.es:doc/5bbc68f9b750603269e812b9
network_acronym_str ES
network_name_str España
repository_id_str
spelling Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in SpainRiaño, I. [0000-0001-8410-2706]Moreno, M.A. [0000-0002-5357-3684]Teshager, T.Sáenz, Y. [0000-0002-2457-4258]Domínguez, L. [0000-0001-8843-0010]Torres, C. [0000-0003-3709-1690]CTX-M-9ESBLsIntegronsS. entericaSHV-12Objectives: To carry out the characterization of the genes encoding extended-spectrum β-lactamases (ESBLs) and their genetic environments in four expanded-spectrum cephalosporin-resistant Salmonella enterica isolates (serovars: two Virchow, one Enteritidis, one Rissen) recovered during the monitoring programmes performed in Spain by the VAV Network from faecal samples of pigs, poultry and laying hens at the slaughterhouse level. Methods: The presence and characterization of ESBL genes as well as their genetic environments in the four S. enterica isolates were investigated by PCR and sequencing. The presence of other resistance genes was also analysed by PCR and sequencing. Results: Three avian S. enterica isolates (two Virchow and one Enteritidis) harboured the blaCTX-M-9 gene combined with blaTEM-1b. The blaCTX-M-9 gene was included in these three isolates in a class 1 integron with the following 5′→3′ structure: integron 1 variable region (dfrA16-aadA2 gene cassettes)-qacEΔ1-sul1-orf 513-blaCTX-M-9-orf3-like- orf1005. The sul2 gene was also detected in these three blaCTX-M-9-containing isolates and tet (A) in one of them. The two serovar Virchow isolates showed an indistinguishable PFGE pattern, although they were recovered from different animal species (broiler and laying hen). A porcine ESBL-positive isolate (serovar Rissen) harboured the blaSHV-12 gene combined with blaTEM-1b. This blaSHV-12 -containing isolate also harboured the tet (A), aadA and sul1 genes. Conclusions: The emergence of ESBL-producing S. enterica isolates among food animals is described for the first time in Spain, with those of the CTX-M group being the predominant ESBLs detected. © 2006 Oxford University Press.2006info:eu-repo/semantics/articleSubtype: Articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://investigacion.unirioja.es/documentos/5bbc68f9b750603269e812b9reponame:RIUR. Repositorio Institucional de la Universidad de La Riojainstname:Universidad de La Rioja (UR)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1093/JAC/DKL337info:eu-repo/semantics/altIdentifier/wos/WOS:000240587400022info:eu-repo/semantics/altIdentifier/pmid/16935865info:eu-repo/semantics/altIdentifier/pissn/0305-7453Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain, 2006, vol. 58, núm. 4, pág. 844-847info:eu-repo/semantics/openAccessoai:portal.dialnet.es:doc/5bbc68f9b750603269e812b92026-06-14T12:47:17Z
dc.title.none.fl_str_mv Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain
title Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain
spellingShingle Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain
Riaño, I. [0000-0001-8410-2706]
CTX-M-9
ESBLs
Integrons
S. enterica
SHV-12
title_short Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain
title_full Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain
title_fullStr Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain
title_full_unstemmed Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain
title_sort Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain
dc.creator.none.fl_str_mv Riaño, I. [0000-0001-8410-2706]
Moreno, M.A. [0000-0002-5357-3684]
Teshager, T.
Sáenz, Y. [0000-0002-2457-4258]
Domínguez, L. [0000-0001-8843-0010]
Torres, C. [0000-0003-3709-1690]
author Riaño, I. [0000-0001-8410-2706]
author_facet Riaño, I. [0000-0001-8410-2706]
Moreno, M.A. [0000-0002-5357-3684]
Teshager, T.
Sáenz, Y. [0000-0002-2457-4258]
Domínguez, L. [0000-0001-8843-0010]
Torres, C. [0000-0003-3709-1690]
author_role author
author2 Moreno, M.A. [0000-0002-5357-3684]
Teshager, T.
Sáenz, Y. [0000-0002-2457-4258]
Domínguez, L. [0000-0001-8843-0010]
Torres, C. [0000-0003-3709-1690]
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CTX-M-9
ESBLs
Integrons
S. enterica
SHV-12
topic CTX-M-9
ESBLs
Integrons
S. enterica
SHV-12
description Objectives: To carry out the characterization of the genes encoding extended-spectrum β-lactamases (ESBLs) and their genetic environments in four expanded-spectrum cephalosporin-resistant Salmonella enterica isolates (serovars: two Virchow, one Enteritidis, one Rissen) recovered during the monitoring programmes performed in Spain by the VAV Network from faecal samples of pigs, poultry and laying hens at the slaughterhouse level. Methods: The presence and characterization of ESBL genes as well as their genetic environments in the four S. enterica isolates were investigated by PCR and sequencing. The presence of other resistance genes was also analysed by PCR and sequencing. Results: Three avian S. enterica isolates (two Virchow and one Enteritidis) harboured the blaCTX-M-9 gene combined with blaTEM-1b. The blaCTX-M-9 gene was included in these three isolates in a class 1 integron with the following 5′→3′ structure: integron 1 variable region (dfrA16-aadA2 gene cassettes)-qacEΔ1-sul1-orf 513-blaCTX-M-9-orf3-like- orf1005. The sul2 gene was also detected in these three blaCTX-M-9-containing isolates and tet (A) in one of them. The two serovar Virchow isolates showed an indistinguishable PFGE pattern, although they were recovered from different animal species (broiler and laying hen). A porcine ESBL-positive isolate (serovar Rissen) harboured the blaSHV-12 gene combined with blaTEM-1b. This blaSHV-12 -containing isolate also harboured the tet (A), aadA and sul1 genes. Conclusions: The emergence of ESBL-producing S. enterica isolates among food animals is described for the first time in Spain, with those of the CTX-M group being the predominant ESBLs detected. © 2006 Oxford University Press.
publishDate 2006
dc.date.none.fl_str_mv 2006
dc.type.none.fl_str_mv info:eu-repo/semantics/article
Subtype: Article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://investigacion.unirioja.es/documentos/5bbc68f9b750603269e812b9
url https://investigacion.unirioja.es/documentos/5bbc68f9b750603269e812b9
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/JAC/DKL337
info:eu-repo/semantics/altIdentifier/wos/WOS:000240587400022
info:eu-repo/semantics/altIdentifier/pmid/16935865
info:eu-repo/semantics/altIdentifier/pissn/0305-7453
Detection and characterization of Extended-Spectrum beta-lactamases (ESBLs) in Salmonella enterica strains of healthy food animals in Spain, 2006, vol. 58, núm. 4, pág. 844-847
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIUR. Repositorio Institucional de la Universidad de La Rioja
instname:Universidad de La Rioja (UR)
instname_str Universidad de La Rioja (UR)
reponame_str RIUR. Repositorio Institucional de la Universidad de La Rioja
collection RIUR. Repositorio Institucional de la Universidad de La Rioja
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412670408491008
score 15,300719

Similares en LA Referencia