Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.

[EN]Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) is the main infectious disease of newborn calves. The first step of infection involves bacterial attachment to the intestinal mucosa. This adhesion is mediated by fimbriae that recognize some glycoconjugates on the host cell surface, in...

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Detalhes bibliográficos
Autores: Martín Martín, María Jesús, Martín-Sosa, Samuel, Alonso, Josefa M, Hueso, Pablo
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2003
País:España
Recursos:Universidad de Salamanca (USAL)
Repositório:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/161814
Acesso em linha:http://hdl.handle.net/10366/161814
Access Level:Acesso embargado
Palavra-chave:Bacterial Toxins
Gangliosides
Ceramide
K99 antigen
Cattle
Escherichia coli
Antigens
Cattle Diseases
Ceramides
Escherichia coli Infections
Molecular Structure
Diarrhea
G(M3) Ganglioside
Bacterial Adhesion
Carbohydrate Sequence
Milk
Animals
2403 Bioquímica
leche
gangliósidos
toxinas bacterianas
bovinos
estructura molecular
adhesión bacteriana
gangliósido G(M3)
enfermedades de los bovinos
ceramidas
animales
secuencia de carbohidratos
diarrea
infecciones por Escherichia coli
antígenos
id ES_88dcdf515f3f754a65f2ff33762d7165
oai_identifier_str oai:gredos.usal.es:10366/161814
network_acronym_str ES
network_name_str España
repository_id_str
spelling Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.Martín Martín, María JesúsMartín-Sosa, SamuelAlonso, Josefa MHueso, PabloBacterial ToxinsGangliosidesCeramideK99 antigenCattleEscherichia coliAntigensCattle DiseasesCeramidesEscherichia coli InfectionsMolecular StructureBacterial ToxinsDiarrheaGangliosidesG(M3) GangliosideBacterial AdhesionCarbohydrate SequenceMilkAnimals2403 Bioquímicalechegangliósidostoxinas bacterianasbovinosestructura molecularadhesión bacterianagangliósido G(M3)enfermedades de los bovinosceramidasanimalessecuencia de carbohidratosEscherichia colidiarreainfecciones por Escherichia coliantígenos[EN]Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) is the main infectious disease of newborn calves. The first step of infection involves bacterial attachment to the intestinal mucosa. This adhesion is mediated by fimbriae that recognize some glycoconjugates on the host cell surface, in particular, several gangliosides. Because milk also contains gangliosides, these have been suggested to serve as ligands for bacterial fimbriae and thus prevent the bacterial attachment to mucosa. The most relevant ETEC strains in calves, including those with K99 and F41 fimbriae, were assayed to determine whether they are able to bind gangliosides isolated from several stages of bovine lactation. Both GM3 and GD3, the main gangliosides of milk, were recognized by ETEC strains, although the different fimbriae showed diverse levels of affinity. Unexpectedly, the adhesion to colostral gangliosides was considerably weaker than that to gangliosides from the other stages of lactation. Because the carbohydrate moiety did not change and because differences in the percentages of unsaturated FA and sphingosine between colostrum and other stages were observed, we conclude that the differences in adhesion could be due to a different composition of the ganglioside ceramide.Wileyinfo202520252003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/161814reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésPrograma de Apoyo a Proyectos de Investigación de la Junta de Castilla y León, España (SA093/01)Attribution-NonCommercial-NoDerivs 3.0 Unportedhttp://creativecommons.org/licenses/by-nc-nd/3.0/info:eu-repo/semantics/embargoedAccessoai:gredos.usal.es:10366/1618142026-06-07T06:28:51Z
dc.title.none.fl_str_mv Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.
title Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.
spellingShingle Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.
Martín Martín, María Jesús
Bacterial Toxins
Gangliosides
Ceramide
K99 antigen
Cattle
Escherichia coli
Antigens
Cattle Diseases
Ceramides
Escherichia coli Infections
Molecular Structure
Bacterial Toxins
Diarrhea
Gangliosides
G(M3) Ganglioside
Bacterial Adhesion
Carbohydrate Sequence
Milk
Animals
2403 Bioquímica
leche
gangliósidos
toxinas bacterianas
bovinos
estructura molecular
adhesión bacteriana
gangliósido G(M3)
enfermedades de los bovinos
ceramidas
animales
secuencia de carbohidratos
Escherichia coli
diarrea
infecciones por Escherichia coli
antígenos
title_short Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.
title_full Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.
title_fullStr Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.
title_full_unstemmed Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.
title_sort Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.
dc.creator.none.fl_str_mv Martín Martín, María Jesús
Martín-Sosa, Samuel
Alonso, Josefa M
Hueso, Pablo
author Martín Martín, María Jesús
author_facet Martín Martín, María Jesús
Martín-Sosa, Samuel
Alonso, Josefa M
Hueso, Pablo
author_role author
author2 Martín-Sosa, Samuel
Alonso, Josefa M
Hueso, Pablo
author2_role author
author
author
dc.subject.none.fl_str_mv Bacterial Toxins
Gangliosides
Ceramide
K99 antigen
Cattle
Escherichia coli
Antigens
Cattle Diseases
Ceramides
Escherichia coli Infections
Molecular Structure
Bacterial Toxins
Diarrhea
Gangliosides
G(M3) Ganglioside
Bacterial Adhesion
Carbohydrate Sequence
Milk
Animals
2403 Bioquímica
leche
gangliósidos
toxinas bacterianas
bovinos
estructura molecular
adhesión bacteriana
gangliósido G(M3)
enfermedades de los bovinos
ceramidas
animales
secuencia de carbohidratos
Escherichia coli
diarrea
infecciones por Escherichia coli
antígenos
topic Bacterial Toxins
Gangliosides
Ceramide
K99 antigen
Cattle
Escherichia coli
Antigens
Cattle Diseases
Ceramides
Escherichia coli Infections
Molecular Structure
Bacterial Toxins
Diarrhea
Gangliosides
G(M3) Ganglioside
Bacterial Adhesion
Carbohydrate Sequence
Milk
Animals
2403 Bioquímica
leche
gangliósidos
toxinas bacterianas
bovinos
estructura molecular
adhesión bacteriana
gangliósido G(M3)
enfermedades de los bovinos
ceramidas
animales
secuencia de carbohidratos
Escherichia coli
diarrea
infecciones por Escherichia coli
antígenos
description [EN]Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) is the main infectious disease of newborn calves. The first step of infection involves bacterial attachment to the intestinal mucosa. This adhesion is mediated by fimbriae that recognize some glycoconjugates on the host cell surface, in particular, several gangliosides. Because milk also contains gangliosides, these have been suggested to serve as ligands for bacterial fimbriae and thus prevent the bacterial attachment to mucosa. The most relevant ETEC strains in calves, including those with K99 and F41 fimbriae, were assayed to determine whether they are able to bind gangliosides isolated from several stages of bovine lactation. Both GM3 and GD3, the main gangliosides of milk, were recognized by ETEC strains, although the different fimbriae showed diverse levels of affinity. Unexpectedly, the adhesion to colostral gangliosides was considerably weaker than that to gangliosides from the other stages of lactation. Because the carbohydrate moiety did not change and because differences in the percentages of unsaturated FA and sphingosine between colostrum and other stages were observed, we conclude that the differences in adhesion could be due to a different composition of the ganglioside ceramide.
publishDate 2003
dc.date.none.fl_str_mv 2003
2025
2025
info
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/161814
url http://hdl.handle.net/10366/161814
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Programa de Apoyo a Proyectos de Investigación de la Junta de Castilla y León, España (SA093/01)
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivs 3.0 Unported
http://creativecommons.org/licenses/by-nc-nd/3.0/
info:eu-repo/semantics/embargoedAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivs 3.0 Unported
http://creativecommons.org/licenses/by-nc-nd/3.0/
eu_rights_str_mv embargoedAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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