Enterotoxigenic Escherichia coli strains bind bovine milk gangliosides in a ceramide-dependent process.
[EN]Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) is the main infectious disease of newborn calves. The first step of infection involves bacterial attachment to the intestinal mucosa. This adhesion is mediated by fimbriae that recognize some glycoconjugates on the host cell surface, in...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2003 |
| País: | España |
| Institución: | Universidad de Salamanca (USAL) |
| Repositorio: | GREDOS. Repositorio Institucional de la Universidad de Salamanca |
| OAI Identifier: | oai:gredos.usal.es:10366/161814 |
| Acceso en línea: | http://hdl.handle.net/10366/161814 |
| Access Level: | acceso embargado |
| Palabra clave: | Bacterial Toxins Gangliosides Ceramide K99 antigen Cattle Escherichia coli Antigens Cattle Diseases Ceramides Escherichia coli Infections Molecular Structure Diarrhea G(M3) Ganglioside Bacterial Adhesion Carbohydrate Sequence Milk Animals 2403 Bioquímica leche gangliósidos toxinas bacterianas bovinos estructura molecular adhesión bacteriana gangliósido G(M3) enfermedades de los bovinos ceramidas animales secuencia de carbohidratos diarrea infecciones por Escherichia coli antígenos |
| Sumario: | [EN]Diarrhea caused by enterotoxigenic Escherichia coli (ETEC) is the main infectious disease of newborn calves. The first step of infection involves bacterial attachment to the intestinal mucosa. This adhesion is mediated by fimbriae that recognize some glycoconjugates on the host cell surface, in particular, several gangliosides. Because milk also contains gangliosides, these have been suggested to serve as ligands for bacterial fimbriae and thus prevent the bacterial attachment to mucosa. The most relevant ETEC strains in calves, including those with K99 and F41 fimbriae, were assayed to determine whether they are able to bind gangliosides isolated from several stages of bovine lactation. Both GM3 and GD3, the main gangliosides of milk, were recognized by ETEC strains, although the different fimbriae showed diverse levels of affinity. Unexpectedly, the adhesion to colostral gangliosides was considerably weaker than that to gangliosides from the other stages of lactation. Because the carbohydrate moiety did not change and because differences in the percentages of unsaturated FA and sphingosine between colostrum and other stages were observed, we conclude that the differences in adhesion could be due to a different composition of the ganglioside ceramide. |
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