Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development

Cytokinin signalling plays both positive and negative roles in plant resistance to pathogens. It is not clear whether the role ofcytokinin changes at the different stages of pathogen infection. Arabidopsis thaliana sequentially exhibits distinct root mor-phological symptoms during Ralstonia solanace...

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Autores: Wang, Xiang, Gong, Qichang, Cheng, Shengyang, Qin, Ning, Cao, Tao, Chen, Yue, Wang, Dongdong, Valls, Marc, Coll, Núria S., Chen, Qin, Zhao, Cuizhu, Lu, Haibin
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/376188
Acesso em linha:http://hdl.handle.net/10261/376188
https://api.elsevier.com/content/abstract/scopus_id/85213016256
Access Level:acceso abierto
Palavra-chave:Bacterial wilt
Cytokinin
Plant immunity
Ralstonia solanacearum
Root disease symptoms
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spelling Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease DevelopmentWang, XiangGong, QichangCheng, ShengyangQin, NingCao, TaoChen, YueWang, DongdongValls, MarcColl, Núria S.Chen, QinZhao, CuizhuLu, HaibinBacterial wiltCytokininPlant immunityRalstonia solanacearumRoot disease symptomsCytokinin signalling plays both positive and negative roles in plant resistance to pathogens. It is not clear whether the role ofcytokinin changes at the different stages of pathogen infection. Arabidopsis thaliana sequentially exhibits distinct root mor-phological symptoms during Ralstonia solanacearum infection, which offers a good system to investigate function of cytokininin the whole pathogen infection process. Using this system, we found increase of cytokinin signalling by Lonely Guy 2 (LOG2)overexpression or depletion of type-A Arabidopsis Response Regulators (ARRs), negative regulators of cytokinin signalling path-way, promoted cell death, wilting symptom and bacterial growth, but attenuated primary root growth inhibition and lateral rootformation. The decrease of cytokinin signalling by mutation on Isopentenyl Transferases (IPTs) inhibited root hair formation, celldeath, wilting symptom and bacterial colonisation. Application of different concentration of exogenesis 6-benzylaminopurine(6-BA) showed first promoted, then decreased root hair formation. Moreover, application of 6-BA accelerated cell death but sup-pressed lateral root formation and primary root growth inhibition. The diverse roles of cytokinin in these different root diseasephenotypes suggested function of cytokinin during plant responses to R. solanacearum is cell type-specific, which provides newinsights on roles of cytokinin signalling in regulation on plant–pathogen interactions.The work was supported by the Nature Science Basic Research Plan of Shaanxi Province (2024JC-YBMS-144) for Haibin Lu, the Fundamental Research Fund of Northwest A&F University Seeds Industry Innovation (Z1090322151) for Haibin Lu, the Special Research Project from Shaanxi province (F2020221001) for Haibin Lu, the Program of Introducing Talents of Innovative Discipline to Universities (Project 111) from the State Administration of Foreign Experts Affairs (B18042) for Haibin Lu and the Natural Science Foundation of Shaanxi Province, China (2024JC-YBMS-180) for Cuizhu Zhao.Peer reviewedJohn Wiley & SonsShaanxi ProvinceNorthwest A&F UniversityState Administration of Foreign Experts Affairs (China)Natural Science Foundation of Shaanxi ProvinceValls, Marc [0000-0003-2312-0091]Lu, Haibin [0000-0002-0613-4697]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/376188https://api.elsevier.com/content/abstract/scopus_id/85213016256reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1111/mpp.70045https://doi.org/10.1111/mpp.70045Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3761882026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development
title Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development
spellingShingle Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development
Wang, Xiang
Bacterial wilt
Cytokinin
Plant immunity
Ralstonia solanacearum
Root disease symptoms
title_short Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development
title_full Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development
title_fullStr Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development
title_full_unstemmed Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development
title_sort Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development
dc.creator.none.fl_str_mv Wang, Xiang
Gong, Qichang
Cheng, Shengyang
Qin, Ning
Cao, Tao
Chen, Yue
Wang, Dongdong
Valls, Marc
Coll, Núria S.
Chen, Qin
Zhao, Cuizhu
Lu, Haibin
author Wang, Xiang
author_facet Wang, Xiang
Gong, Qichang
Cheng, Shengyang
Qin, Ning
Cao, Tao
Chen, Yue
Wang, Dongdong
Valls, Marc
Coll, Núria S.
Chen, Qin
Zhao, Cuizhu
Lu, Haibin
author_role author
author2 Gong, Qichang
Cheng, Shengyang
Qin, Ning
Cao, Tao
Chen, Yue
Wang, Dongdong
Valls, Marc
Coll, Núria S.
Chen, Qin
Zhao, Cuizhu
Lu, Haibin
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Shaanxi Province
Northwest A&F University
State Administration of Foreign Experts Affairs (China)
Natural Science Foundation of Shaanxi Province
Valls, Marc [0000-0003-2312-0091]
Lu, Haibin [0000-0002-0613-4697]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacterial wilt
Cytokinin
Plant immunity
Ralstonia solanacearum
Root disease symptoms
topic Bacterial wilt
Cytokinin
Plant immunity
Ralstonia solanacearum
Root disease symptoms
description Cytokinin signalling plays both positive and negative roles in plant resistance to pathogens. It is not clear whether the role ofcytokinin changes at the different stages of pathogen infection. Arabidopsis thaliana sequentially exhibits distinct root mor-phological symptoms during Ralstonia solanacearum infection, which offers a good system to investigate function of cytokininin the whole pathogen infection process. Using this system, we found increase of cytokinin signalling by Lonely Guy 2 (LOG2)overexpression or depletion of type-A Arabidopsis Response Regulators (ARRs), negative regulators of cytokinin signalling path-way, promoted cell death, wilting symptom and bacterial growth, but attenuated primary root growth inhibition and lateral rootformation. The decrease of cytokinin signalling by mutation on Isopentenyl Transferases (IPTs) inhibited root hair formation, celldeath, wilting symptom and bacterial colonisation. Application of different concentration of exogenesis 6-benzylaminopurine(6-BA) showed first promoted, then decreased root hair formation. Moreover, application of 6-BA accelerated cell death but sup-pressed lateral root formation and primary root growth inhibition. The diverse roles of cytokinin in these different root diseasephenotypes suggested function of cytokinin during plant responses to R. solanacearum is cell type-specific, which provides newinsights on roles of cytokinin signalling in regulation on plant–pathogen interactions.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/376188
https://api.elsevier.com/content/abstract/scopus_id/85213016256
url http://hdl.handle.net/10261/376188
https://api.elsevier.com/content/abstract/scopus_id/85213016256
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1111/mpp.70045
https://doi.org/10.1111/mpp.70045
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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