Cytokinin Plays a Multifaceted Role in Ralstonia solanacearum-Triggered Plant Disease Development

Cytokinin signalling plays both positive and negative roles in plant resistance to pathogens. It is not clear whether the role ofcytokinin changes at the different stages of pathogen infection. Arabidopsis thaliana sequentially exhibits distinct root mor-phological symptoms during Ralstonia solanace...

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Detalles Bibliográficos
Autores: Wang, Xiang, Gong, Qichang, Cheng, Shengyang, Qin, Ning, Cao, Tao, Chen, Yue, Wang, Dongdong, Valls, Marc, Coll, Núria S., Chen, Qin, Zhao, Cuizhu, Lu, Haibin
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/376188
Acceso en línea:http://hdl.handle.net/10261/376188
https://api.elsevier.com/content/abstract/scopus_id/85213016256
Access Level:acceso abierto
Palabra clave:Bacterial wilt
Cytokinin
Plant immunity
Ralstonia solanacearum
Root disease symptoms
Descripción
Sumario:Cytokinin signalling plays both positive and negative roles in plant resistance to pathogens. It is not clear whether the role ofcytokinin changes at the different stages of pathogen infection. Arabidopsis thaliana sequentially exhibits distinct root mor-phological symptoms during Ralstonia solanacearum infection, which offers a good system to investigate function of cytokininin the whole pathogen infection process. Using this system, we found increase of cytokinin signalling by Lonely Guy 2 (LOG2)overexpression or depletion of type-A Arabidopsis Response Regulators (ARRs), negative regulators of cytokinin signalling path-way, promoted cell death, wilting symptom and bacterial growth, but attenuated primary root growth inhibition and lateral rootformation. The decrease of cytokinin signalling by mutation on Isopentenyl Transferases (IPTs) inhibited root hair formation, celldeath, wilting symptom and bacterial colonisation. Application of different concentration of exogenesis 6-benzylaminopurine(6-BA) showed first promoted, then decreased root hair formation. Moreover, application of 6-BA accelerated cell death but sup-pressed lateral root formation and primary root growth inhibition. The diverse roles of cytokinin in these different root diseasephenotypes suggested function of cytokinin during plant responses to R. solanacearum is cell type-specific, which provides newinsights on roles of cytokinin signalling in regulation on plant–pathogen interactions.