Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans

Compounds promoting anabolic effects on muscle and bone may offer an ideal treatment for osteosarcopenia while potentially impacting healthspan and lifespan. We previously demonstrated the anabolic effects of picolinic acid (PIC), a tryptophan metabolite, on bone both in vitro and in vivo. However,...

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Autores: Rivas , Daniel, Alokda , Abdelrahman, Al Saedi , Ahmed, Karasik , David, Van Raamsdonk , Jeremy M, Duque , Gustavo, Baltasar Fernández, Iván
Formato: artículo
Fecha de publicación:2025
País:España
Recursos:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/46212
Acesso em linha:https://academic.oup.com/biomedgerontology/advance-article/doi/10.1093/gerona/glaf239/8314215?login=true
https://hdl.handle.net/10578/46212
Access Level:acceso abierto
Palavra-chave:Aging
C. Elegans
Gerotherapeutics
Healthspan
Kynurenine
Tryptophan
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spelling Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegansRivas , DanielAlokda , AbdelrahmanAl Saedi , AhmedKarasik , DavidVan Raamsdonk , Jeremy MDuque , GustavoBaltasar Fernández, IvánAgingC. ElegansGerotherapeuticsHealthspanKynurenineTryptophanCompounds promoting anabolic effects on muscle and bone may offer an ideal treatment for osteosarcopenia while potentially impacting healthspan and lifespan. We previously demonstrated the anabolic effects of picolinic acid (PIC), a tryptophan metabolite, on bone both in vitro and in vivo. However, its effects on muscle and potential additional effects on lifespan and healthspan are not yet fully understood. This study aimed to investigate PICs effects on muscle cells in vitro and its impact on mobility and lifespan in an animal model. Murine C2C12 and human myoblasts were treated with PIC (1, 50 and 100 µM) or vehicle for 5 days. Myogenic regulatory factors (MRFs) were evaluated, and the fusion index and myotubules’ length were calculated at timed intervals (Day 1, 3, and 5). In vivo, Caenorhabditis elegans were treated with increasing doses of PIC, and their lifespan and rate of movement (thrashing) were evaluated at timed intervals. PIC-treated myoblasts showed a higher and earlier expression of MRFs. On day 3, PIC-treated myotubes were significantly more fused and longer when treated with PIC than vehicle-treated controls. C. elegans treated with 1 mM of PIC showed a significantly longer lifespan. In addition, the mobility of PIC-treated C. elegans was significantly increased at all timed points. In conclusion, this study demonstrates that, besides its anabolic effect on bone, PIC has an anabolic effect on muscle, which is also associated with a longer lifespan in PIC-treated C. elegans. This evidence opens up promising avenues for further exploration of PIC as a novel therapy for osteosarcopenia with additional effects on healthspan and lifespan.Oxford Academic202620262025info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://academic.oup.com/biomedgerontology/advance-article/doi/10.1093/gerona/glaf239/8314215?login=truehttps://hdl.handle.net/10578/46212reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésCIHR—Grant 525642FEDER Funds from the European Union (2022-GRIN-34296)info:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/462122026-05-27T07:36:41Z
dc.title.none.fl_str_mv Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
title Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
spellingShingle Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
Rivas , Daniel
Aging
C. Elegans
Gerotherapeutics
Healthspan
Kynurenine
Tryptophan
title_short Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
title_full Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
title_fullStr Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
title_full_unstemmed Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
title_sort Picolinic acid, a tryptophan metabolite, exhibits anabolic effects in muscle cells and improves lifespan and movement in C. elegans
dc.creator.none.fl_str_mv Rivas , Daniel
Alokda , Abdelrahman
Al Saedi , Ahmed
Karasik , David
Van Raamsdonk , Jeremy M
Duque , Gustavo
Baltasar Fernández, Iván
author Rivas , Daniel
author_facet Rivas , Daniel
Alokda , Abdelrahman
Al Saedi , Ahmed
Karasik , David
Van Raamsdonk , Jeremy M
Duque , Gustavo
Baltasar Fernández, Iván
author_role author
author2 Alokda , Abdelrahman
Al Saedi , Ahmed
Karasik , David
Van Raamsdonk , Jeremy M
Duque , Gustavo
Baltasar Fernández, Iván
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Aging
C. Elegans
Gerotherapeutics
Healthspan
Kynurenine
Tryptophan
topic Aging
C. Elegans
Gerotherapeutics
Healthspan
Kynurenine
Tryptophan
description Compounds promoting anabolic effects on muscle and bone may offer an ideal treatment for osteosarcopenia while potentially impacting healthspan and lifespan. We previously demonstrated the anabolic effects of picolinic acid (PIC), a tryptophan metabolite, on bone both in vitro and in vivo. However, its effects on muscle and potential additional effects on lifespan and healthspan are not yet fully understood. This study aimed to investigate PICs effects on muscle cells in vitro and its impact on mobility and lifespan in an animal model. Murine C2C12 and human myoblasts were treated with PIC (1, 50 and 100 µM) or vehicle for 5 days. Myogenic regulatory factors (MRFs) were evaluated, and the fusion index and myotubules’ length were calculated at timed intervals (Day 1, 3, and 5). In vivo, Caenorhabditis elegans were treated with increasing doses of PIC, and their lifespan and rate of movement (thrashing) were evaluated at timed intervals. PIC-treated myoblasts showed a higher and earlier expression of MRFs. On day 3, PIC-treated myotubes were significantly more fused and longer when treated with PIC than vehicle-treated controls. C. elegans treated with 1 mM of PIC showed a significantly longer lifespan. In addition, the mobility of PIC-treated C. elegans was significantly increased at all timed points. In conclusion, this study demonstrates that, besides its anabolic effect on bone, PIC has an anabolic effect on muscle, which is also associated with a longer lifespan in PIC-treated C. elegans. This evidence opens up promising avenues for further exploration of PIC as a novel therapy for osteosarcopenia with additional effects on healthspan and lifespan.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://academic.oup.com/biomedgerontology/advance-article/doi/10.1093/gerona/glaf239/8314215?login=true
https://hdl.handle.net/10578/46212
url https://academic.oup.com/biomedgerontology/advance-article/doi/10.1093/gerona/glaf239/8314215?login=true
https://hdl.handle.net/10578/46212
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv CIHR—Grant 525642
FEDER Funds from the European Union (2022-GRIN-34296)
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford Academic
publisher.none.fl_str_mv Oxford Academic
dc.source.none.fl_str_mv reponame:RUIdeRA. Repositorio Institucional de la UCLM
instname:Universidad de Castilla-La Mancha
instname_str Universidad de Castilla-La Mancha
reponame_str RUIdeRA. Repositorio Institucional de la UCLM
collection RUIdeRA. Repositorio Institucional de la UCLM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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