Synonymous genome recoding

Synthetic genome recoding is a new means of generating designed organisms with altered phenotypes. Synonymous mutations introduced into the protein coding region tolerate modifications in DNA or mRNA without modifying the encoded proteins. Synonymous genome-wide recoding has allowed the synthetic ge...

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Autores: Martínez, Miguel Angel, Jordan de Paiz, Ana|||0000-0002-3495-7201, Franco Cirera, Sandra|||0000-0003-4126-2202, Nevot Banús, Maria|||0000-0003-4947-4648
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:226633
Acceso en línea:https://ddd.uab.cat/record/226633
https://dx.doi.org/urn:doi:10.1093/nar/gkz831
Access Level:acceso abierto
Palabra clave:Animals
Communicable Disease Control, methods
Genome, Bacterial
Genome, Viral
Genomics, methods
Humans
Mutagenesis
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spelling Synonymous genome recodinga tool to explore microbial biology and new therapeutic strategiesMartínez, Miguel AngelJordan de Paiz, Ana|||0000-0002-3495-7201Franco Cirera, Sandra|||0000-0003-4126-2202Nevot Banús, Maria|||0000-0003-4947-4648AnimalsCommunicable Disease Control, methodsGenome, BacterialGenome, ViralGenomics, methodsHumansMutagenesisSynthetic genome recoding is a new means of generating designed organisms with altered phenotypes. Synonymous mutations introduced into the protein coding region tolerate modifications in DNA or mRNA without modifying the encoded proteins. Synonymous genome-wide recoding has allowed the synthetic generation of different small-genome viruses with modified phenotypes and biological properties. Recently, a decreased cost of chemically synthesizing DNA and improved methods for assembling DNA fragments (e.g. lambda red recombination and CRISPR-based editing) have enabled the construction of an Escherichia coli variant with a 4-Mb synthetic synonymously recoded genome with a reduced number of sense codons (n = 59) encoding the 20 canonical amino acids. Synonymous genome recoding is increasing our knowledge of microbial interactions with innate immune responses, identifying functional genome structures, and strategically ameliorating cis-inhibitory signaling sequences related to splicing, replication (in eukaryotes), and complex microbe functions, unraveling the relevance of codon usage for the temporal regulation of gene expression and the microbe mutant spectrum and adaptability. New biotechnological and therapeutic applications of this methodology can easily be envisaged. In this review, we discuss how synonymous genome recoding may impact our knowledge of microbial biology and the development of new and better therapeutic methodologies.Universitat Autònoma de Barcelona 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/226633https://dx.doi.org/urn:doi:10.1093/nar/gkz831reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2016-75277-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BES-2014-069931Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 RD16-0025-0041open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2266332026-06-06T12:50:31Z
dc.title.none.fl_str_mv Synonymous genome recoding
a tool to explore microbial biology and new therapeutic strategies
title Synonymous genome recoding
spellingShingle Synonymous genome recoding
Martínez, Miguel Angel
Animals
Communicable Disease Control, methods
Genome, Bacterial
Genome, Viral
Genomics, methods
Humans
Mutagenesis
title_short Synonymous genome recoding
title_full Synonymous genome recoding
title_fullStr Synonymous genome recoding
title_full_unstemmed Synonymous genome recoding
title_sort Synonymous genome recoding
dc.creator.none.fl_str_mv Martínez, Miguel Angel
Jordan de Paiz, Ana|||0000-0002-3495-7201
Franco Cirera, Sandra|||0000-0003-4126-2202
Nevot Banús, Maria|||0000-0003-4947-4648
author Martínez, Miguel Angel
author_facet Martínez, Miguel Angel
Jordan de Paiz, Ana|||0000-0002-3495-7201
Franco Cirera, Sandra|||0000-0003-4126-2202
Nevot Banús, Maria|||0000-0003-4947-4648
author_role author
author2 Jordan de Paiz, Ana|||0000-0002-3495-7201
Franco Cirera, Sandra|||0000-0003-4126-2202
Nevot Banús, Maria|||0000-0003-4947-4648
author2_role author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Animals
Communicable Disease Control, methods
Genome, Bacterial
Genome, Viral
Genomics, methods
Humans
Mutagenesis
topic Animals
Communicable Disease Control, methods
Genome, Bacterial
Genome, Viral
Genomics, methods
Humans
Mutagenesis
description Synthetic genome recoding is a new means of generating designed organisms with altered phenotypes. Synonymous mutations introduced into the protein coding region tolerate modifications in DNA or mRNA without modifying the encoded proteins. Synonymous genome-wide recoding has allowed the synthetic generation of different small-genome viruses with modified phenotypes and biological properties. Recently, a decreased cost of chemically synthesizing DNA and improved methods for assembling DNA fragments (e.g. lambda red recombination and CRISPR-based editing) have enabled the construction of an Escherichia coli variant with a 4-Mb synthetic synonymously recoded genome with a reduced number of sense codons (n = 59) encoding the 20 canonical amino acids. Synonymous genome recoding is increasing our knowledge of microbial interactions with innate immune responses, identifying functional genome structures, and strategically ameliorating cis-inhibitory signaling sequences related to splicing, replication (in eukaryotes), and complex microbe functions, unraveling the relevance of codon usage for the temporal regulation of gene expression and the microbe mutant spectrum and adaptability. New biotechnological and therapeutic applications of this methodology can easily be envisaged. In this review, we discuss how synonymous genome recoding may impact our knowledge of microbial biology and the development of new and better therapeutic methodologies.
publishDate 2019
dc.date.none.fl_str_mv 2
2019-01-01
2019
2019-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/226633
https://dx.doi.org/urn:doi:10.1093/nar/gkz831
url https://ddd.uab.cat/record/226633
https://dx.doi.org/urn:doi:10.1093/nar/gkz831
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 SAF2016-75277-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BES-2014-069931
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 RD16-0025-0041
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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