Response of thawed epidi dymal red deer spermatozoa to increasing concentrations of hydrogen peroxide, and importance of individual male variability
Oxidative stress represents a challenge during sperm manipulation. We have tested the effect of increasing hydrogen peroxide (H2O2) levels on red deer spermatozoa after cryopreservation, and the role of male-to-male variation in that response. In a first experiment, eight thawed samples were submitt...
| Autores: | , , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2011 |
| País: | España |
| Institución: | Universidad de Castilla-La Mancha |
| Repositorio: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:ruidera.uclm.es:10578/7459 |
| Acceso en línea: | http://hdl.handle.net/10578/7459 |
| Access Level: | acceso abierto |
| Palabra clave: | Ciervos Espermatozoides Estrés oxidativo Agua oxigenada |
| Sumario: | Oxidative stress represents a challenge during sperm manipulation. We have tested the effect of increasing hydrogen peroxide (H2O2) levels on red deer spermatozoa after cryopreservation, and the role of male-to-male variation in that response. In a first experiment, eight thawed samples were submitted to 0, 25, 50, 100 and 200 μM H2O2 for 2 h at 37 °C. Intracellular ROS (H2DCFDA-CM) increased with H2O2 concentration, but we only detected a decrease in sperm function (motility by CASA and chromatin damage by SCSA) with 200 μM. Lipoperoxidation (TBARS) increased slightly with 50 μM H2O2 and above. In a second experiment, samples from 7 males were submitted to 0 and 200 μM H2O2 for 2 h, triplicating the experiment within each male. Males differed at thawing and regarding their response to incubation and H2O2 presence. We found that the kinematic parameters reflected male-to-male variability, whereas the response of the different males was similar for lipid peroxidation and viability. A multiparametric analysis showed that males grouped differently if samples were assessed after thawing, after incubation without H2O2, or after incubation with H2O2. Red deer spermatozoa are relatively resilient to H2O2 after thawing, but it seems to be a great male-to-male variability regarding the response to oxidative stress. The acknowledgement of this individual variability might improve the development of optimized sperm work protocols. |
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