Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggs

This research investigates the potential of lactic acid bacteria (LAB) from freshwater salmonids as prospective probiotics for application in aquaculture. LAB and pathogenic bacteria were obtained from mucus and tissues of Oncorhynchus mykiss and Salmo trutta from fish farms in northeast Spain that...

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Autores: Vargas González, Augusto, Barajas Vélez, Miguel Ángel, Pérez Sánchez, Tania
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/47980
Acceso en línea:https://hdl.handle.net/2454/47980
Access Level:acceso abierto
Palabra clave:Antagonistic assays
Antimicrobial resistance
Embryonated eggs
Fish pathogens
Lactic acid bacteria
Probiotics
Salmo trutta
Salmonids
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spelling Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggsVargas González, AugustoBarajas Vélez, Miguel ÁngelPérez Sánchez, TaniaAntagonistic assaysAntimicrobial resistanceEmbryonated eggsFish pathogensLactic acid bacteriaProbioticsSalmo truttaSalmonidsThis research investigates the potential of lactic acid bacteria (LAB) from freshwater salmonids as prospective probiotics for application in aquaculture. LAB and pathogenic bacteria were obtained from mucus and tissues of Oncorhynchus mykiss and Salmo trutta from fish farms in northeast Spain that had not used antibiotics for the six months preceding the study. Isolates were identified using Gram staining and sequencing of 16S rRNA and ITS-1. To assess the safety of the LAB, antibiotic susceptibility tests (ASTs) against 23 antimicrobials were performed. In vitro antagonism assays were conducted to evaluate the inhibitory effects of living LAB using the agar diffusion test method and their metabolites using the agar well diffusion method. The assays targeted six specific pathogens: Aeromonas salmonicida subsp. salmonicida, Carnobacterium maltaromaticum, Vagococcus salmoninarum, Yersinia ruckeri, Lactococcus garvieae, and the marine pathogen Vibrio jasicida. Additionally, a toxicity assay was conducted on embryonic eggs of S. trutta. The ASTs on probiotic LAB candidates revealed varied responses to antimicrobials, but no resistance to oxytetracycline or florfenicol, which are two antibiotics commonly used in aquaculture, was detected. The in vitro assays indicate that LAB exhibit antagonistic effects against pathogens, primarily when directly stimulated by their presence. In applications involving embryonic eggs or larvae, certain live strains of LAB were found to have adverse effects, with some isolates resulting in higher mortality rates compared to the control group or other isolates. Furthermore, the potential pathogenicity of certain LAB strains, typically considered safe in salmonids, warrants deeper investigation.This research was conducted with internal funding provided by the research team members. Additionally, A.V.-G. acknowledges the support of a predoctoral grant from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 801586, managed by Campus Iberus/Universidad Pública de Navarra.MDPICiencias de la SaludOsasun Zientziak2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/47980reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/801586© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/479802026-06-17T12:41:47Z
dc.title.none.fl_str_mv Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggs
title Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggs
spellingShingle Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggs
Vargas González, Augusto
Antagonistic assays
Antimicrobial resistance
Embryonated eggs
Fish pathogens
Lactic acid bacteria
Probiotics
Salmo trutta
Salmonids
title_short Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggs
title_full Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggs
title_fullStr Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggs
title_full_unstemmed Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggs
title_sort Isolation of Lactic Acid Bacteria (LAB) from salmonids for potential use as probiotics: in vitro assays and toxicity assessment of salmo trutta embryonated eggs
dc.creator.none.fl_str_mv Vargas González, Augusto
Barajas Vélez, Miguel Ángel
Pérez Sánchez, Tania
author Vargas González, Augusto
author_facet Vargas González, Augusto
Barajas Vélez, Miguel Ángel
Pérez Sánchez, Tania
author_role author
author2 Barajas Vélez, Miguel Ángel
Pérez Sánchez, Tania
author2_role author
author
dc.contributor.none.fl_str_mv Ciencias de la Salud
Osasun Zientziak
dc.subject.none.fl_str_mv Antagonistic assays
Antimicrobial resistance
Embryonated eggs
Fish pathogens
Lactic acid bacteria
Probiotics
Salmo trutta
Salmonids
topic Antagonistic assays
Antimicrobial resistance
Embryonated eggs
Fish pathogens
Lactic acid bacteria
Probiotics
Salmo trutta
Salmonids
description This research investigates the potential of lactic acid bacteria (LAB) from freshwater salmonids as prospective probiotics for application in aquaculture. LAB and pathogenic bacteria were obtained from mucus and tissues of Oncorhynchus mykiss and Salmo trutta from fish farms in northeast Spain that had not used antibiotics for the six months preceding the study. Isolates were identified using Gram staining and sequencing of 16S rRNA and ITS-1. To assess the safety of the LAB, antibiotic susceptibility tests (ASTs) against 23 antimicrobials were performed. In vitro antagonism assays were conducted to evaluate the inhibitory effects of living LAB using the agar diffusion test method and their metabolites using the agar well diffusion method. The assays targeted six specific pathogens: Aeromonas salmonicida subsp. salmonicida, Carnobacterium maltaromaticum, Vagococcus salmoninarum, Yersinia ruckeri, Lactococcus garvieae, and the marine pathogen Vibrio jasicida. Additionally, a toxicity assay was conducted on embryonic eggs of S. trutta. The ASTs on probiotic LAB candidates revealed varied responses to antimicrobials, but no resistance to oxytetracycline or florfenicol, which are two antibiotics commonly used in aquaculture, was detected. The in vitro assays indicate that LAB exhibit antagonistic effects against pathogens, primarily when directly stimulated by their presence. In applications involving embryonic eggs or larvae, certain live strains of LAB were found to have adverse effects, with some isolates resulting in higher mortality rates compared to the control group or other isolates. Furthermore, the potential pathogenicity of certain LAB strains, typically considered safe in salmonids, warrants deeper investigation.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/47980
url https://hdl.handle.net/2454/47980
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/European Commission/Horizon 2020 Framework Programme/801586
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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