Integrated process for sequential extraction of bioactive phenolic compounds and proteins from mill and field olive leaves and effects on the lignocellulosic profile

The extraction of bioactive compounds in a biorefinery context could be a way to valorize agri-food byproducts, but there is a remaining part that also requires attention. Therefore, in this work the integrated extraction of phenolic compounds, including the bioactive oleuropein, and proteins from o...

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Detalles Bibliográficos
Autores: Contreras, María Del Mar, Lama Muñoz, Antonio, Gutiérrez Pérez, José Luis, Espínola, Francisco, Moya, Manuel, Romero, Inmaculada, Castro, Eulogio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/169035
Acceso en línea:https://hdl.handle.net/11441/169035
https://doi.org/10.3390/foods8110531
Access Level:acceso abierto
Palabra clave:Bioactive compounds
Biorefinery
Oleuropein
Olive leaves
Phenolic compounds
Vegetable protein
Ultrasound
Descripción
Sumario:The extraction of bioactive compounds in a biorefinery context could be a way to valorize agri-food byproducts, but there is a remaining part that also requires attention. Therefore, in this work the integrated extraction of phenolic compounds, including the bioactive oleuropein, and proteins from olive mill leaves was addressed following three schemes, including the use of ultrasound. This a ected the total phenolic content (4475.5–6166.9 mg gallic acid equivalents/100 g), oleuropein content (675.3–1790.0 mg/100 g), and antioxidant activity (18,234.3–25,459.0 mol trolox equivalents/100 g). No e ect was observed on either the protein recovery or the content of sugars and lignin in the extraction residues. Concerning the recovery of proteins, three operational parameters were evaluated by response surface methodology. The optimum (63.1%) was achieved using NaOH 0.7 M at 100 C for 240 min. Then, the selected scheme was applied to olive leaves from the f ield, observing di erences in the content of some of the studied components. It also changed the lignocellulosic profile of the extraction residues of both leaf types, which were enriched in cellulose. Overall, these results could be useful to diversify the valorization chain in the olive sector.