Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows

Background/Objectives: Classical swine fever (CSF) continues to challenge global eradication efforts, particularly in endemic regions, where pregnant sows face heightened risks of vertical transmission following exposure to CSFV. Methods: This study evaluates the early protective efficacy of FlagT4G...

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Autores: Coronado, Liani, Cobos, Àlex, Muñoz-Aguilera, Adriana, Puente Marin, Sara, Guevara, Gemma, Riquelme, Cristina, Heredia, Saray, Borca, Manuel V., Ganges, Llilianne
Formato: artículo
Fecha de publicación:2025
País:España
Recursos:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:dnet:irtapubpro__::64e973f844306dcccfdc925cd29a841b
Acesso em linha:http://hdl.handle.net/20.500.12327/4798
https://doi.org/10.3390/vaccines13080803
Access Level:acceso abierto
Palavra-chave:619
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spelling Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant SowsCoronado, LianiCobos, ÀlexMuñoz-Aguilera, AdrianaPuente Marin, SaraGuevara, GemmaRiquelme, CristinaHeredia, SarayBorca, Manuel V.Ganges, Llilianne619Background/Objectives: Classical swine fever (CSF) continues to challenge global eradication efforts, particularly in endemic regions, where pregnant sows face heightened risks of vertical transmission following exposure to CSFV. Methods: This study evaluates the early protective efficacy of FlagT4G, a novel live attenuated DIVA-compatible vaccine. Pregnant sows were vaccinated at mid-gestation and challenged 14 days later with a highly virulent CSFV strain. Results: FlagT4G conferred complete clinical protection, preventing both maternal viremia and transplacental transmission. No CSFV RNA, specific antibodies, or IFN-α were detected in fetal samples from vaccinated animals. In contrast, unvaccinated sows exhibited clinical signs, high viral loads, and widespread fetal infection. Interestingly, early protection was observed even in the absence of strong humoral responses in some vaccinated sows, suggesting a potential role for innate or T-cell-mediated immunity in conferring rapid protection. Conclusions: The demonstrated efficacy of FlagT4G within two weeks of vaccination underscores its feasibility for integration into emergency vaccination programs. Its DIVA compatibility and ability to induce early fetal protection against highly virulent CSFV strains position it as a promising tool for CSF control and eradication strategies.This research was funded by USDA-IRTA Agreement No. NACA 58-8064-0-013F and the Spanish Ministry of Science and Innovation, grant PID2021-125599OB-100. L.C. was supported by the Juan de la Cierva Program (2022), Spanish Ministry of Science and Innovation. A.M.-A. was supported by the ICA grant 001-2022. The IRTA is supported by the CERCA program/Generalitat de Catalunya.info:eu-repo/semantics/publishedVersionMDPIProducció AnimalSanitat Animal202520252025info:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/20.500.12327/4798https://doi.org/10.3390/vaccines13080803reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésVaccinesMICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-125599OB-I00/ES/FACTORES DEL VIRUS Y DEL HOSPEDADOR COMO DIANAS PARA EL DISEÑO DE UNA NUEVA ESTRATEGIA DIVA FRENTE A LA PESTE PORCINA CLÁSICA/Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:irtapubpro__::64e973f844306dcccfdc925cd29a841b2026-06-16T08:51:17Z
dc.title.none.fl_str_mv Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows
title Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows
spellingShingle Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows
Coronado, Liani
619
title_short Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows
title_full Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows
title_fullStr Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows
title_full_unstemmed Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows
title_sort Protection Against Transplacental Transmission of a Highly Virulent Classical Swine Fever Virus Two Weeks After Single-Dose FlagT4G Vaccination in Pregnant Sows
dc.creator.none.fl_str_mv Coronado, Liani
Cobos, Àlex
Muñoz-Aguilera, Adriana
Puente Marin, Sara
Guevara, Gemma
Riquelme, Cristina
Heredia, Saray
Borca, Manuel V.
Ganges, Llilianne
author Coronado, Liani
author_facet Coronado, Liani
Cobos, Àlex
Muñoz-Aguilera, Adriana
Puente Marin, Sara
Guevara, Gemma
Riquelme, Cristina
Heredia, Saray
Borca, Manuel V.
Ganges, Llilianne
author_role author
author2 Cobos, Àlex
Muñoz-Aguilera, Adriana
Puente Marin, Sara
Guevara, Gemma
Riquelme, Cristina
Heredia, Saray
Borca, Manuel V.
Ganges, Llilianne
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Sanitat Animal
dc.subject.none.fl_str_mv 619
topic 619
description Background/Objectives: Classical swine fever (CSF) continues to challenge global eradication efforts, particularly in endemic regions, where pregnant sows face heightened risks of vertical transmission following exposure to CSFV. Methods: This study evaluates the early protective efficacy of FlagT4G, a novel live attenuated DIVA-compatible vaccine. Pregnant sows were vaccinated at mid-gestation and challenged 14 days later with a highly virulent CSFV strain. Results: FlagT4G conferred complete clinical protection, preventing both maternal viremia and transplacental transmission. No CSFV RNA, specific antibodies, or IFN-α were detected in fetal samples from vaccinated animals. In contrast, unvaccinated sows exhibited clinical signs, high viral loads, and widespread fetal infection. Interestingly, early protection was observed even in the absence of strong humoral responses in some vaccinated sows, suggesting a potential role for innate or T-cell-mediated immunity in conferring rapid protection. Conclusions: The demonstrated efficacy of FlagT4G within two weeks of vaccination underscores its feasibility for integration into emergency vaccination programs. Its DIVA compatibility and ability to induce early fetal protection against highly virulent CSFV strains position it as a promising tool for CSF control and eradication strategies.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/4798
https://doi.org/10.3390/vaccines13080803
url http://hdl.handle.net/20.500.12327/4798
https://doi.org/10.3390/vaccines13080803
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Vaccines
MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2021-125599OB-I00/ES/FACTORES DEL VIRUS Y DEL HOSPEDADOR COMO DIANAS PARA EL DISEÑO DE UNA NUEVA ESTRATEGIA DIVA FRENTE A LA PESTE PORCINA CLÁSICA/
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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