Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorization

13p.-4 fig.-5 tab.

Detalles Bibliográficos
Autores: Méndez-Líter, Juan A., Eugenio, Laura I. de, Hakalin, Neumara L. S., Prieto Orzanco, Alicia, Martínez, María Jesús
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/263444
Acceso en línea:http://hdl.handle.net/10261/263444
Access Level:acceso abierto
Palabra clave:Biodiesel by-products
Cellulases
Fungi
Hydrolases
Wastes
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spelling Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorizationMéndez-Líter, Juan A.Eugenio, Laura I. deHakalin, Neumara L. S.Prieto Orzanco, AliciaMartínez, María JesúsBiodiesel by-productsCellulasesFungiHydrolasesWastes13p.-4 fig.-5 tab.As β-glucosidases represent the major bottleneck for the industrial degradation of plant biomass, great efforts are being devoted to discover both novel and robust versions of these enzymes, as well as to develop efficient and inexpensive ways to produce them. In this work, raw glycerol from chemical production of biodiesel was tested as carbon source for the fungus Talaromyces amestolkiae with the aim of producing enzyme β-glucosidase-enriched cocktails. Approximately 11 U/mL β-glucosidase was detected in these cultures, constituting the major cellulolytic activity. Proteomic analysis showed BGL-3 as the most abundant protein and the main β-glucosidase. This crude enzyme was successfully used to supplement a basal commercial cellulolytic cocktail (Celluclast 1.5 L) for saccharification of pretreated wheat straw, corroborating that even hardly exploitable industrial wastes, such as glycerol, can be used as secondary raw materials to produce valuable enzymatic preparations in a framework of the circular economy.This work has been funded by Projects GLYSUS RTI2018-093683-Β-I00 (MCIU/AEI/FEDER), RETOPROSOST2 S2018/EMT-4459 (Comunidad de Madrid) and BioSFerA EU-H2020-NMBP-TR-IND.Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Comunidad de MadridEuropean CommissionEugenio, Laura I. de [0000-0002-0496-8663]Hakalin, Neumara L. S. [0000-0002-9027-013X]Prieto, Alicia [0000-0002-5075-4025]Martínez, María Jesús [0000-0003-2166-1097]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/263444reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#S2018/EMT-4459/RETOPROSOST2https://doi.org/10.3390/jof7050363Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2634442026-05-22T06:33:51Z
dc.title.none.fl_str_mv Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorization
title Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorization
spellingShingle Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorization
Méndez-Líter, Juan A.
Biodiesel by-products
Cellulases
Fungi
Hydrolases
Wastes
title_short Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorization
title_full Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorization
title_fullStr Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorization
title_full_unstemmed Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorization
title_sort Production of a β-glucosidase-rich cocktail from talaromyces amestolkiae using raw glycerol: Its role for lignocellulose waste valorization
dc.creator.none.fl_str_mv Méndez-Líter, Juan A.
Eugenio, Laura I. de
Hakalin, Neumara L. S.
Prieto Orzanco, Alicia
Martínez, María Jesús
author Méndez-Líter, Juan A.
author_facet Méndez-Líter, Juan A.
Eugenio, Laura I. de
Hakalin, Neumara L. S.
Prieto Orzanco, Alicia
Martínez, María Jesús
author_role author
author2 Eugenio, Laura I. de
Hakalin, Neumara L. S.
Prieto Orzanco, Alicia
Martínez, María Jesús
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Comunidad de Madrid
European Commission
Eugenio, Laura I. de [0000-0002-0496-8663]
Hakalin, Neumara L. S. [0000-0002-9027-013X]
Prieto, Alicia [0000-0002-5075-4025]
Martínez, María Jesús [0000-0003-2166-1097]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biodiesel by-products
Cellulases
Fungi
Hydrolases
Wastes
topic Biodiesel by-products
Cellulases
Fungi
Hydrolases
Wastes
description 13p.-4 fig.-5 tab.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/263444
url http://hdl.handle.net/10261/263444
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
S2018/EMT-4459/RETOPROSOST2
https://doi.org/10.3390/jof7050363
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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