In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivation

Bioorthogonal metallocatalysis has opened up a xenobiotic route to perform nonenzymatic catalytic transformations in living settings. Despite their promising features, most metals are deactivated inside cells by a myriad of reactive biomolecules, including biogenic thiols, thereby limiting the catal...

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Detalhes bibliográficos
Autores: Rubio‐Ruiz, Belén, Pérez‐López, Ana M., Usón, Laura, Ortega-Liébana, M. Carmen, Valero, Teresa, Arruebo, Manuel, Hueso, José L., Sebastián, Víctor, Santamaría, Jesús, Unciti-Broceta, Asier
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/329906
Acesso em linha:http://hdl.handle.net/10261/329906
Access Level:acceso abierto
Palavra-chave:Palladium
Gold
Nanoalloys
Catalysis
Bioorthogonal
Nanoencapsulation
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spelling In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivationRubio‐Ruiz, BelénPérez‐López, Ana M.Usón, LauraOrtega-Liébana, M. CarmenValero, TeresaArruebo, ManuelHueso, José L.Sebastián, VíctorSantamaría, JesúsUnciti-Broceta, AsierPalladiumGoldNanoalloysCatalysisBioorthogonalNanoencapsulationBioorthogonal metallocatalysis has opened up a xenobiotic route to perform nonenzymatic catalytic transformations in living settings. Despite their promising features, most metals are deactivated inside cells by a myriad of reactive biomolecules, including biogenic thiols, thereby limiting the catalytic functioning of these abiotic reagents. Here we report the development of cytocompatible alloyed AuPd nanoparticles with the capacity to elicit bioorthogonal depropargylations with high efficiency in biological media. We also show that the intracellular catalytic performance of these nanoalloys is significantly enhanced by protecting them following two different encapsulation methods. Encapsulation in mesoporous silica nanorods resulted in augmented catalyst reactivity, whereas the use of a biodegradable PLGA matrix increased nanoalloy delivery across the cell membrane. The functional potential of encapsulated AuPd was demonstrated by releasing the potent chemotherapy drug paclitaxel inside cancer cells. Nanoalloy encapsulation provides a novel methodology to develop nanoreactors capable of mediating new-to-life reactions in cells.We thank the confocal microscopy facilities of the IGC for helping with the experiments [CIBER-BBN (initiative funded by the VI National R&D&i Plan 2008–2011, Iniciativa Ingenio 2010, Consolider Program, CIBER Actions and financed by the Instituto de Salud Carlos III with assistance from the European Regional Development Fund]. The synthesis of materials has been performed by the Platform of Production of Biomaterials and Nanoparticles of the NANBIOSIS ICTS, more specifically by the Nanoparticle Synthesis Unit of the CIBER in BioEngineering, Biomaterials & Nanomedicine (CIBER-BBN). ELECMI (LMA-UNIZAR) ICTS is also gratefully acknowledged.Peer reviewedAmerican Chemical SocietyInstituto de Salud Carlos IIIEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/329906reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1021/acs.nanolett.2c03593Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3299062026-05-22T06:33:51Z
dc.title.none.fl_str_mv In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivation
title In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivation
spellingShingle In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivation
Rubio‐Ruiz, Belén
Palladium
Gold
Nanoalloys
Catalysis
Bioorthogonal
Nanoencapsulation
title_short In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivation
title_full In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivation
title_fullStr In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivation
title_full_unstemmed In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivation
title_sort In cellulo bioorthogonal catalysis by encapsulated AuPd nanoalloys: Overcoming intracellular deactivation
dc.creator.none.fl_str_mv Rubio‐Ruiz, Belén
Pérez‐López, Ana M.
Usón, Laura
Ortega-Liébana, M. Carmen
Valero, Teresa
Arruebo, Manuel
Hueso, José L.
Sebastián, Víctor
Santamaría, Jesús
Unciti-Broceta, Asier
author Rubio‐Ruiz, Belén
author_facet Rubio‐Ruiz, Belén
Pérez‐López, Ana M.
Usón, Laura
Ortega-Liébana, M. Carmen
Valero, Teresa
Arruebo, Manuel
Hueso, José L.
Sebastián, Víctor
Santamaría, Jesús
Unciti-Broceta, Asier
author_role author
author2 Pérez‐López, Ana M.
Usón, Laura
Ortega-Liébana, M. Carmen
Valero, Teresa
Arruebo, Manuel
Hueso, José L.
Sebastián, Víctor
Santamaría, Jesús
Unciti-Broceta, Asier
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Palladium
Gold
Nanoalloys
Catalysis
Bioorthogonal
Nanoencapsulation
topic Palladium
Gold
Nanoalloys
Catalysis
Bioorthogonal
Nanoencapsulation
description Bioorthogonal metallocatalysis has opened up a xenobiotic route to perform nonenzymatic catalytic transformations in living settings. Despite their promising features, most metals are deactivated inside cells by a myriad of reactive biomolecules, including biogenic thiols, thereby limiting the catalytic functioning of these abiotic reagents. Here we report the development of cytocompatible alloyed AuPd nanoparticles with the capacity to elicit bioorthogonal depropargylations with high efficiency in biological media. We also show that the intracellular catalytic performance of these nanoalloys is significantly enhanced by protecting them following two different encapsulation methods. Encapsulation in mesoporous silica nanorods resulted in augmented catalyst reactivity, whereas the use of a biodegradable PLGA matrix increased nanoalloy delivery across the cell membrane. The functional potential of encapsulated AuPd was demonstrated by releasing the potent chemotherapy drug paclitaxel inside cancer cells. Nanoalloy encapsulation provides a novel methodology to develop nanoreactors capable of mediating new-to-life reactions in cells.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/329906
url http://hdl.handle.net/10261/329906
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1021/acs.nanolett.2c03593
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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