Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons

Moreau, Matthieu X.; Saillour, Yoann; Elorriaga, Vicente; Bouloudi, Benoît; Delberghe, Elodie; Deutsch Guerrero, Tanya; Ochandorena Saa, Amaia; Maeso Alonso, Laura; Marqués Martínez, Margarita; Marín Vieira, María Carmen; Spassky, Nathalie; Pierani, Alessandra; Causeret, Frédéric

Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons

[EN] Cajal-Retzius cells (CRs) are key players in cerebral cortex development, and they display a unique transcriptomic identity. Here, we use scRNA-seq to reconstruct the differentiation trajectory of mouse hem-derived CRs, and we unravel the transient expression of a complete gene module previousl...

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Authors:	Moreau, Matthieu X., Saillour, Yoann, Elorriaga, Vicente, Bouloudi, Benoît, Delberghe, Elodie, Deutsch Guerrero, Tanya, Ochandorena Saa, Amaia, Maeso Alonso, Laura, Marqués Martínez, Margarita, Marín Vieira, María Carmen, Spassky, Nathalie, Pierani, Alessandra, Causeret, Frédéric
Format:	article
Status:	Versión aceptada para publicación
Publication Date:	2023
Country:	España
Institution:	Ajuntament de Barcelona
Repository:	BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:	oai:buleria.unileon.es:10612/23632
Online Access:	https://www.sciencedirect.com/science/article/pii/S1534580723002459?via%3Dihub https://hdl.handle.net/10612/23632
Access Level:	Open access
Keyword:	Biología Biotecnología Genética Cajal-Retzius cells Cerebral cortex Multiciliogenesis Neuronal differentiation Neuronal identity Fate specification Choroid plexus 2407 Biología Celular 3109.02 Genética 24 Ciencias de la Vida 240108 Genética Animal 240701 Cultivo Celular 2409 Genética 2415 Biología Molecular

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oai_identifier_str	oai:buleria.unileon.es:10612/23632
network_acronym_str	ES
network_name_str	España
repository_id_str
spelling	Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neuronsMoreau, Matthieu X.Saillour, YoannElorriaga, VicenteBouloudi, BenoîtDelberghe, ElodieDeutsch Guerrero, TanyaOchandorena Saa, AmaiaMaeso Alonso, LauraMarqués Martínez, MargaritaMarín Vieira, María CarmenSpassky, NathaliePierani, AlessandraCauseret, FrédéricBiologíaBiotecnologíaGenéticaCajal-Retzius cellsCerebral cortexMulticiliogenesisNeuronal differentiationNeuronal identityFate specificationChoroid plexus2407 Biología Celular3109.02 Genética24 Ciencias de la Vida240108 Genética Animal2407 Biología Celular240701 Cultivo Celular2409 Genética2415 Biología Molecular2415 Biología Molecular[EN] Cajal-Retzius cells (CRs) are key players in cerebral cortex development, and they display a unique transcriptomic identity. Here, we use scRNA-seq to reconstruct the differentiation trajectory of mouse hem-derived CRs, and we unravel the transient expression of a complete gene module previously known to control multiciliogenesis. However, CRs do not undergo centriole amplification or multiciliation. Upon deletion of Gmnc, the master regulator of multiciliogenesis, CRs are initially produced but fail to reach their normal identity resulting in their massive apoptosis. We further dissect the contribution of multiciliation effector genes and identify Trp73 as a key determinant. Finally, we use in utero electroporation to demonstrate that the intrinsic competence of hem progenitors as well as the heterochronic expression of Gmnc prevent centriole amplification in the CR lineage. Our work exemplifies how the co-option of a complete gene module, repurposed to control a distinct process, may contribute to the emergence of novel cell identitiesSICell Press; Elsevier BVBiologia CelularFacultad de Ciencias Biologicas y Ambientales2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttps://www.sciencedirect.com/science/article/pii/S1534580723002459?via%3Dihubhttps://hdl.handle.net/10612/23632reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Ajuntament de BarcelonaIngléshttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/236322026-06-24T12:43:27Z
dc.title.none.fl_str_mv	Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons
title	Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons
spellingShingle	Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons Moreau, Matthieu X. Biología Biotecnología Genética Cajal-Retzius cells Cerebral cortex Multiciliogenesis Neuronal differentiation Neuronal identity Fate specification Choroid plexus 2407 Biología Celular 3109.02 Genética 24 Ciencias de la Vida 240108 Genética Animal 2407 Biología Celular 240701 Cultivo Celular 2409 Genética 2415 Biología Molecular 2415 Biología Molecular
title_short	Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons
title_full	Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons
title_fullStr	Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons
title_full_unstemmed	Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons
title_sort	Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons
dc.creator.none.fl_str_mv	Moreau, Matthieu X. Saillour, Yoann Elorriaga, Vicente Bouloudi, Benoît Delberghe, Elodie Deutsch Guerrero, Tanya Ochandorena Saa, Amaia Maeso Alonso, Laura Marqués Martínez, Margarita Marín Vieira, María Carmen Spassky, Nathalie Pierani, Alessandra Causeret, Frédéric
author	Moreau, Matthieu X.
author_facet	Moreau, Matthieu X. Saillour, Yoann Elorriaga, Vicente Bouloudi, Benoît Delberghe, Elodie Deutsch Guerrero, Tanya Ochandorena Saa, Amaia Maeso Alonso, Laura Marqués Martínez, Margarita Marín Vieira, María Carmen Spassky, Nathalie Pierani, Alessandra Causeret, Frédéric
author_role	author
author2	Saillour, Yoann Elorriaga, Vicente Bouloudi, Benoît Delberghe, Elodie Deutsch Guerrero, Tanya Ochandorena Saa, Amaia Maeso Alonso, Laura Marqués Martínez, Margarita Marín Vieira, María Carmen Spassky, Nathalie Pierani, Alessandra Causeret, Frédéric
author2_role	author author author author author author author author author author author author
dc.contributor.none.fl_str_mv	Biologia Celular Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv	Biología Biotecnología Genética Cajal-Retzius cells Cerebral cortex Multiciliogenesis Neuronal differentiation Neuronal identity Fate specification Choroid plexus 2407 Biología Celular 3109.02 Genética 24 Ciencias de la Vida 240108 Genética Animal 2407 Biología Celular 240701 Cultivo Celular 2409 Genética 2415 Biología Molecular 2415 Biología Molecular
topic	Biología Biotecnología Genética Cajal-Retzius cells Cerebral cortex Multiciliogenesis Neuronal differentiation Neuronal identity Fate specification Choroid plexus 2407 Biología Celular 3109.02 Genética 24 Ciencias de la Vida 240108 Genética Animal 2407 Biología Celular 240701 Cultivo Celular 2409 Genética 2415 Biología Molecular 2415 Biología Molecular
description	[EN] Cajal-Retzius cells (CRs) are key players in cerebral cortex development, and they display a unique transcriptomic identity. Here, we use scRNA-seq to reconstruct the differentiation trajectory of mouse hem-derived CRs, and we unravel the transient expression of a complete gene module previously known to control multiciliogenesis. However, CRs do not undergo centriole amplification or multiciliation. Upon deletion of Gmnc, the master regulator of multiciliogenesis, CRs are initially produced but fail to reach their normal identity resulting in their massive apoptosis. We further dissect the contribution of multiciliation effector genes and identify Trp73 as a key determinant. Finally, we use in utero electroporation to demonstrate that the intrinsic competence of hem progenitors as well as the heterochronic expression of Gmnc prevent centriole amplification in the CR lineage. Our work exemplifies how the co-option of a complete gene module, repurposed to control a distinct process, may contribute to the emergence of novel cell identities
publishDate	2023
dc.date.none.fl_str_mv	2023
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion
format	article
status_str	acceptedVersion
dc.identifier.none.fl_str_mv	https://www.sciencedirect.com/science/article/pii/S1534580723002459?via%3Dihub https://hdl.handle.net/10612/23632
url	https://www.sciencedirect.com/science/article/pii/S1534580723002459?via%3Dihub https://hdl.handle.net/10612/23632
dc.language.none.fl_str_mv	Inglés
language_invalid_str_mv	Inglés
dc.rights.none.fl_str_mv	http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess
rights_invalid_str_mv	http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Cell Press; Elsevier BV
publisher.none.fl_str_mv	Cell Press; Elsevier BV
dc.source.none.fl_str_mv	reponame:BULERIA. Repositorio Institucional de la Universidad de León instname:Ajuntament de Barcelona
instname_str	Ajuntament de Barcelona
reponame_str	BULERIA. Repositorio Institucional de la Universidad de León
collection	BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_	1869411911074840576
score	15,811543

Repurposing of the multiciliation gene regulatory network in fate specification of Cajal-Retzius neurons

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