Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis

© 2021 by the authors.

Detalles Bibliográficos
Autores: Marrero, M., Monroig, Óscar, Betancor, Mónica B., Herrera, M., Pérez, José A., Garrido, Diego, Galindo, A., Giráldez, I., Rodríguez, Covadonga
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/239701
Acceso en línea:http://hdl.handle.net/10261/239701
Access Level:acceso abierto
Palabra clave:Solea senegalensis
Fads2
Elovl5
LC-PUFA biosynthesis
Diet
Salinity
EPA
DHA
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spelling Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensisMarrero, M.Monroig, ÓscarBetancor, Mónica B.Herrera, M.Pérez, José A.Garrido, DiegoGalindo, A.Giráldez, I.Rodríguez, CovadongaSolea senegalensisFads2Elovl5LC-PUFA biosynthesisDietSalinityEPADHA© 2021 by the authors.Fish vary in their ability to biosynthesise long-chain polyunsaturated fatty acids (LC-PUFA) depending upon the complement and function of key enzymes commonly known as fatty acyl desaturases and elongases. It has been reported in Solea senegalensis the existence of a Δ4 desaturase, enabling the biosynthesis of docosahexaenoic acid (DHA) from eicosapentaenoic acid (EPA), which can be modulated by the diet. The present study aims to evaluate the combined effects of the partial replacement of fish oil (FO) with vegetable oils and reduced environmental salinity in the fatty acid composition of relevant body compartments (muscle, hepatocytes and enterocytes), the enzymatic activity over α-linolenic acid (ALA) to form n-3 LC-PUFA through the incubation of isolated hepatocytes and enterocytes with [1-14C] 18:3 n-3, and the regulation of the S. senegalensis fads2 and elovl5 in the liver and intestine. The presence of radiolabelled products, including 18:4n-3, 20:4n-3 and EPA, provided compelling evidence that a complete pathway enabling the biosynthesis of EPA from ALA, establishing S. senegalensis, has at least one Fads2 with ∆6 activity. Dietary composition prevailed over salinity in regulating the expression of fads2, while salinity did so over dietary composition for elovl5. FO replacement enhanced the proportion of DHA in S. senegalensis muscle and the combination with 20 ppt salinity increased the amount of n-3 LC-PUFA in hepatocytes.This study was funded through the project PROPUFAW3 of the Ministry of Science, Innovation and Universities, Spanish Government (AGL2015-70994-R); Agencia Canaria de Investigación, Innovación y Sociedad de la Información de la Consejería de Economía, Conocimiento y Empleo and Fondo Social Europeo (FSE) Programa Operativo Integrado de Canarias 2014-2020, Eje 3 Tema Prioritario 74 (85%) (PhD contract M.M.); CajaSiete (PhD contract A.G.).Peer reviewedMultidisciplinary Digital Publishing InstituteMinisterio de Economía y Competitividad (España)Agencia Canaria de Investigación, Innovación y Sociedad de la InformaciónEuropean CommissionCajasieteConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/239701reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-70994-Rhttps://doi.org/10.3390/md19050254Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2397012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis
title Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis
spellingShingle Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis
Marrero, M.
Solea senegalensis
Fads2
Elovl5
LC-PUFA biosynthesis
Diet
Salinity
EPA
DHA
title_short Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis
title_full Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis
title_fullStr Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis
title_full_unstemmed Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis
title_sort Influence of Dietary Lipids and Environmental Salinity on the n-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis Capacity of the Marine Teleost Solea senegalensis
dc.creator.none.fl_str_mv Marrero, M.
Monroig, Óscar
Betancor, Mónica B.
Herrera, M.
Pérez, José A.
Garrido, Diego
Galindo, A.
Giráldez, I.
Rodríguez, Covadonga
author Marrero, M.
author_facet Marrero, M.
Monroig, Óscar
Betancor, Mónica B.
Herrera, M.
Pérez, José A.
Garrido, Diego
Galindo, A.
Giráldez, I.
Rodríguez, Covadonga
author_role author
author2 Monroig, Óscar
Betancor, Mónica B.
Herrera, M.
Pérez, José A.
Garrido, Diego
Galindo, A.
Giráldez, I.
Rodríguez, Covadonga
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Canaria de Investigación, Innovación y Sociedad de la Información
European Commission
Cajasiete
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Solea senegalensis
Fads2
Elovl5
LC-PUFA biosynthesis
Diet
Salinity
EPA
DHA
topic Solea senegalensis
Fads2
Elovl5
LC-PUFA biosynthesis
Diet
Salinity
EPA
DHA
description © 2021 by the authors.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/239701
url http://hdl.handle.net/10261/239701
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-70994-R
https://doi.org/10.3390/md19050254
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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