Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation

Resident peritoneal macrophages (resMØs) are crucial for repairing peritoneal injuries and controlling infections by forming mesothelium-bound resMØ-aggre gates in the peritoneal wall and omentum. Here we present a protocol to analyze these structures in mouse models of peritoneal inflammation. We d...

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Authors: Ferriz, Margarita, Vega Pérez, Adrián, Gutiérrez González, Alejandra, Álvarez Ladrón, Natalia, Ardavín, Carlos
Format: article
Publication Date:2023
Country:España
Institution:Universidad Alfonso X el Sabio
Repository:Repositorio Institucional de la Universidad Alfonso X el Sabio
Language:English
OAI Identifier:oai:archive.uax.com:20.500.12080/44938
Online Access:https://hdl.handle.net/20.500.12080/44938
Access Level:Open access
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spelling Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammationFerriz, MargaritaVega Pérez, AdriánGutiérrez González, AlejandraÁlvarez Ladrón, NataliaArdavín, CarlosResident peritoneal macrophages (resMØs) are crucial for repairing peritoneal injuries and controlling infections by forming mesothelium-bound resMØ-aggre gates in the peritoneal wall and omentum. Here we present a protocol to analyze these structures in mouse models of peritoneal inflammation. We describe the dissection, fixation, immunofluorescent staining, and mounting of whole perito neal wall and omentum samples and subsequent confocal microscopy imaging of resMØ-aggregates. We also detail the steps to isolate resMØ-aggregates for additional studies, including flow cytometry and electron-microscopy-based analysis.UAX Archive20232023-03-0120232023-03-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12080/44938reponame:Repositorio Institucional de la Universidad Alfonso X el Sabioinstname:Universidad Alfonso X el SabioInglésengopen accesshttp://purl.org/coar/access_right/c_abf2CC-BYhttp://creativecommons.org/licenses/by/4.0/deed.esinfo:eu-repo/semantics/openAccessoai:archive.uax.com:20.500.12080/449382026-05-28T12:40:21Z
dc.title.none.fl_str_mv Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation
title Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation
spellingShingle Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation
Ferriz, Margarita
title_short Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation
title_full Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation
title_fullStr Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation
title_full_unstemmed Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation
title_sort Whole-mount immunofluorescence imaging and isolation of mesothelium-bound immune cell aggregates during mouse peritoneal inflammation
dc.creator.none.fl_str_mv Ferriz, Margarita
Vega Pérez, Adrián
Gutiérrez González, Alejandra
Álvarez Ladrón, Natalia
Ardavín, Carlos
author Ferriz, Margarita
author_facet Ferriz, Margarita
Vega Pérez, Adrián
Gutiérrez González, Alejandra
Álvarez Ladrón, Natalia
Ardavín, Carlos
author_role author
author2 Vega Pérez, Adrián
Gutiérrez González, Alejandra
Álvarez Ladrón, Natalia
Ardavín, Carlos
author2_role author
author
author
author
dc.contributor.none.fl_str_mv UAX Archive
description Resident peritoneal macrophages (resMØs) are crucial for repairing peritoneal injuries and controlling infections by forming mesothelium-bound resMØ-aggre gates in the peritoneal wall and omentum. Here we present a protocol to analyze these structures in mouse models of peritoneal inflammation. We describe the dissection, fixation, immunofluorescent staining, and mounting of whole perito neal wall and omentum samples and subsequent confocal microscopy imaging of resMØ-aggregates. We also detail the steps to isolate resMØ-aggregates for additional studies, including flow cytometry and electron-microscopy-based analysis.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-03-01
2023
2023-03-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12080/44938
url https://hdl.handle.net/20.500.12080/44938
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
CC-BY
http://creativecommons.org/licenses/by/4.0/deed.es
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
CC-BY
http://creativecommons.org/licenses/by/4.0/deed.es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Institucional de la Universidad Alfonso X el Sabio
instname:Universidad Alfonso X el Sabio
instname_str Universidad Alfonso X el Sabio
reponame_str Repositorio Institucional de la Universidad Alfonso X el Sabio
collection Repositorio Institucional de la Universidad Alfonso X el Sabio
repository.name.fl_str_mv
repository.mail.fl_str_mv
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