Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetin
The aim of this work was to optimize the preparative process of quercetin loaded casein nanoparticles as well as to evaluate the pharmacokinetics of this flavonoid when administered orally in Wistar rats. Nanoparticles were obtained by coacervation after the incubation of casein, 2-hydroxypropyl-β-c...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/61902 |
| Acceso en línea: | https://hdl.handle.net/10171/61902 |
| Access Level: | acceso abierto |
| Palabra clave: | Materias Investigacion::Ciencias de la Salud::Química médica Nanoparticles Cyclodextrin Casein Quercetin Nutraceutical Oral delivery |
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Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetinPeñalva, R. (Rebeca)|||/items/04b1892c-08d1-4c62-b9a3-6bc0340c7a7eEsparza, I. (Irene)|||/items/d3ae94dd-a0bc-4baa-b05f-8ba804fd99e4Morales, J. (Jorge)|||/items/e65f5428-b569-416f-8ca5-c6aac44dfbbaGonzalez-Navarro, C.J. (Carlos Javier)|||/items/480527b9-23db-4625-b9e0-a91f385e9a1aLarrañeta, E. (Eneko)|||/items/dfeb2f8f-aa42-45ef-82fe-d61110bb0166Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173Materias Investigacion::Ciencias de la Salud::Química médicaNanoparticlesCyclodextrinCaseinQuercetinNutraceuticalOral deliveryThe aim of this work was to optimize the preparative process of quercetin loaded casein nanoparticles as well as to evaluate the pharmacokinetics of this flavonoid when administered orally in Wistar rats. Nanoparticles were obtained by coacervation after the incubation of casein, 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and quercetin in an aqueous environment. Then, nanoparticles were purified and dried. The resulting nanoparticles displayed a size of 200 nm with a negative zeta potential and a payload of about 32 μg/mg. Release studies showed a zero-order kinetic, suggesting a mechanism based on erosion of the nanoparticle matrix. For the pharmacokinetic study, quercetin was orally administered to rats as a single dose of 25 mg/kg. Animals treated with quercetin-loaded casein nanoparticles displayed higher plasma levels than those observed in animals receiving the solution of the flavonoid (control). Thus, the relative oral bioavailability of quercetin when administered as casein nanoparticles (close to 37%) was found to be about 9-times higher than the oral solution of the flavonoid in a mixture of PEG 400 and water. In summary, the combination of casein and 2-hydroxypropyl-β-cyclodextrin produces nanoparticles that may be a good option to load quercetin for both nutraceutical and pharmaceutical purposes.Elsevier BVDadun. Depósito Académico Digital Universidad de Navarra20212021-09-0220192019-01-0120192019-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/61902reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/619022026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetin |
| title |
Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetin |
| spellingShingle |
Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetin Peñalva, R. (Rebeca)|||/items/04b1892c-08d1-4c62-b9a3-6bc0340c7a7e Materias Investigacion::Ciencias de la Salud::Química médica Nanoparticles Cyclodextrin Casein Quercetin Nutraceutical Oral delivery |
| title_short |
Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetin |
| title_full |
Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetin |
| title_fullStr |
Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetin |
| title_full_unstemmed |
Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetin |
| title_sort |
Casein nanoparticles in combination with 2-hydroxypropyl-β-cyclodextrin improves the oral bioavailability of quercetin |
| dc.creator.none.fl_str_mv |
Peñalva, R. (Rebeca)|||/items/04b1892c-08d1-4c62-b9a3-6bc0340c7a7e Esparza, I. (Irene)|||/items/d3ae94dd-a0bc-4baa-b05f-8ba804fd99e4 Morales, J. (Jorge)|||/items/e65f5428-b569-416f-8ca5-c6aac44dfbba Gonzalez-Navarro, C.J. (Carlos Javier)|||/items/480527b9-23db-4625-b9e0-a91f385e9a1a Larrañeta, E. (Eneko)|||/items/dfeb2f8f-aa42-45ef-82fe-d61110bb0166 Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173 |
| author |
Peñalva, R. (Rebeca)|||/items/04b1892c-08d1-4c62-b9a3-6bc0340c7a7e |
| author_facet |
Peñalva, R. (Rebeca)|||/items/04b1892c-08d1-4c62-b9a3-6bc0340c7a7e Esparza, I. (Irene)|||/items/d3ae94dd-a0bc-4baa-b05f-8ba804fd99e4 Morales, J. (Jorge)|||/items/e65f5428-b569-416f-8ca5-c6aac44dfbba Gonzalez-Navarro, C.J. (Carlos Javier)|||/items/480527b9-23db-4625-b9e0-a91f385e9a1a Larrañeta, E. (Eneko)|||/items/dfeb2f8f-aa42-45ef-82fe-d61110bb0166 Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173 |
| author_role |
author |
| author2 |
Esparza, I. (Irene)|||/items/d3ae94dd-a0bc-4baa-b05f-8ba804fd99e4 Morales, J. (Jorge)|||/items/e65f5428-b569-416f-8ca5-c6aac44dfbba Gonzalez-Navarro, C.J. (Carlos Javier)|||/items/480527b9-23db-4625-b9e0-a91f385e9a1a Larrañeta, E. (Eneko)|||/items/dfeb2f8f-aa42-45ef-82fe-d61110bb0166 Irache-Garreta, J.M. (Juan Manuel)|||/items/c7cbbe9e-faeb-47e1-b7e8-2d956ca50173 |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Materias Investigacion::Ciencias de la Salud::Química médica Nanoparticles Cyclodextrin Casein Quercetin Nutraceutical Oral delivery |
| topic |
Materias Investigacion::Ciencias de la Salud::Química médica Nanoparticles Cyclodextrin Casein Quercetin Nutraceutical Oral delivery |
| description |
The aim of this work was to optimize the preparative process of quercetin loaded casein nanoparticles as well as to evaluate the pharmacokinetics of this flavonoid when administered orally in Wistar rats. Nanoparticles were obtained by coacervation after the incubation of casein, 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and quercetin in an aqueous environment. Then, nanoparticles were purified and dried. The resulting nanoparticles displayed a size of 200 nm with a negative zeta potential and a payload of about 32 μg/mg. Release studies showed a zero-order kinetic, suggesting a mechanism based on erosion of the nanoparticle matrix. For the pharmacokinetic study, quercetin was orally administered to rats as a single dose of 25 mg/kg. Animals treated with quercetin-loaded casein nanoparticles displayed higher plasma levels than those observed in animals receiving the solution of the flavonoid (control). Thus, the relative oral bioavailability of quercetin when administered as casein nanoparticles (close to 37%) was found to be about 9-times higher than the oral solution of the flavonoid in a mixture of PEG 400 and water. In summary, the combination of casein and 2-hydroxypropyl-β-cyclodextrin produces nanoparticles that may be a good option to load quercetin for both nutraceutical and pharmaceutical purposes. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 2019-01-01 2019 2019-01-01 2021 2021-09-02 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/61902 |
| url |
https://hdl.handle.net/10171/61902 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier BV |
| publisher.none.fl_str_mv |
Elsevier BV |
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reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
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Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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Dadun. Depósito Académico Digital de la Universidad de Navarra |
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15.300719 |