MUC1 aptamer-capped mesoporous silica nanoparticles for controlled drug delivery and radio-imaging applications

[EN] Mucin 1 (MUC1) is a cell surface protein overexpressed in breast cancer. Mesoporous silica nanoparticles (MSNs) loaded with safranin O, functionalized with aminopropyl groups and gated with the negatively charged MUC1 aptamer have been prepared (S1-apMUC1) for specific targeting and cargo relea...

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Detalles Bibliográficos
Autores: Pascual, Lluís, Cerqueira, Cristal, García-Fernández, Alba, de Luis-Fernández, Beatriz, Soares, Emerson, Souza, Marta, Missailidis, Sotiris, Santos-Oliveira, Ralph, Orzaez, Mar, Martínez-Máñez, Ramón|||0000-0001-5873-9674, Sancenón Galarza, Félix|||0000-0002-5205-7135
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/103726
Acceso en línea:https://riunet.upv.es/handle/10251/103726
Access Level:acceso abierto
Palabra clave:Radiopharmaceuticals
Nanotechnology
Oncology
Mesoporous silica nanoparticles
QUIMICA INORGANICA
BIOQUIMICA Y BIOLOGIA MOLECULAR
QUIMICA ORGANICA
Descripción
Sumario:[EN] Mucin 1 (MUC1) is a cell surface protein overexpressed in breast cancer. Mesoporous silica nanoparticles (MSNs) loaded with safranin O, functionalized with aminopropyl groups and gated with the negatively charged MUC1 aptamer have been prepared (S1-apMUC1) for specific targeting and cargo release in tumoral versus non-tumoral cells. Confocal microscopy studies showed that the S1-apMUC1 nanoparticles were internalized in MDA-MB-231 breast cancer cells that overexpress MUC1 receptor with subsequent pore opening and cargo release. Interestingly, the MCF-10-A non-tumorigenic breast epithelial cell line that do not overexpress MUC1, showed reduced (S1-apMUC1) internalization. Negligible internalization was also found for S1-ap nanoparticles that contained a scrambled DNA sequence as gatekeeper. S2-apMUC1 nanoparticles (similar to S1-apMUC1 but loaded with doxorubicin) internalized in MDA-MB-231 cells and induced a remarkable reduction in cell viability. Moreover, S1-apMUC1 nanoparticles radio-labeled with Tc-99m (S1-apMUC1-Tc) showed a remarkable tumor targeting in in vivo studies with MDA-MB-231 tumor-bearing Balb/c mice. (C) 2017 Elsevier Inc. All rights reserved.

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