In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity

[EN] Alginate crosslinked with calcium cations is a promising hydrogel for biomedical applications as it is non-toxic, has suitable mechanical properties and is insoluble in water. Cobalt has been shown to possess antibacterial capacity against Gram-positive and Gram-negative bacteria, and has an an...

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Authors: Cano-Vicent, Alba, Martínez-Agut, Andrea, Tuñón-Molina, Alberto, Bakshi, Hamid, Alfagih, Iman M., Serrano-Aroca, Ángel, Tambuwala, Murtaza M., Sabater i Serra, Roser|||0000-0002-5550-7066
Format: article
Publication Date:2023
Country:España
Institution:Universitat Politècnica de València (UPV)
Repository:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Language:English
OAI Identifier:oai:riunet.upv.es:10251/209931
Online Access:https://riunet.upv.es/handle/10251/209931
Access Level:Open access
Keyword:Alginate
Cobalt
Toxicity
Antiviral
Antibacterial
Anticancer
INGENIERIA ELECTRICA
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spelling In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activityCano-Vicent, AlbaMartínez-Agut, AndreaTuñón-Molina, AlbertoBakshi, HamidAlfagih, Iman M.Serrano-Aroca, ÁngelTambuwala, Murtaza M.Sabater i Serra, Roser|||0000-0002-5550-7066AlginateCobaltToxicityAntiviralAntibacterialAnticancerINGENIERIA ELECTRICA[EN] Alginate crosslinked with calcium cations is a promising hydrogel for biomedical applications as it is non-toxic, has suitable mechanical properties and is insoluble in water. Cobalt has been shown to possess antibacterial capacity against Gram-positive and Gram-negative bacteria, and has an angiogenesis effect. In this study, alginate films were crosslinked with Ca2+ and Co2+ ions to explore their biological properties in terms of antiviral capacity, antibacterial properties, anticancer activity and their toxicity. The results show that the hydrogel with a very small amount of cobalt was biocompatible in vivo using the Caenorhabditis elegans model and in vitro on human keratinocyte cells and it also exhibited antibacterial activity against the life-threatening methicillinresistant Staphylococcus aureus. Furthermore, this hydrogel showed antiviral activity against a surrogate of SARSCoV-2 and anticancer properties against melanoma and colon cancer cells, which render it a promising material for biomedical applications such as wound healing and tissue engineering. Water sorption experiments, Fourier transform infrared spectroscopy, electron microscopy with Energy Dispersive X-ray Spectrometry and degradation analysis in acid aqueous medium were performed to complete the characterization of these new materials.The authors would like to express their gratitude to the Fundacion Universidad Catolica de Valencia San Vicente Martir and to the Spanish Ministry of Science and Innovation for their financial support through Grant 2020-231-006UCV and PID2020-119333RB-I00 / AEI / 10.13039/501100011033, respectively. The CIBER-BBN initiative is funded by the VI National R&D&I Plan 2008 - 2011, Iniciativa Ingenio 2010, Consolider Program. CIBER actions are financed by the Instituto de Salud Carlos III with assistance from the European Regional Development. Funding support also from Researchers Supporting Project number (RSP-2023R782), King Saud University, Riyadh, Saudi Arabia.ElsevierEscuela Técnica Superior de Ingeniería Aeroespacial y Diseño IndustrialDepartamento de Ingeniería EléctricaCentro de Biomateriales e Ingeniería TisularKing Saud UniversityInstituto de Salud Carlos IIIAgencia Estatal de InvestigaciónUniversidad Católica de Valencia San Vicente MártirRepositorio Institucional de la Universitat Politècnica de València Riunet20232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/209931reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-119333RB-I00 SOPORTES BIOFUNCIONALES CON CAPACIDAD OSTEOINDUCTORA Y ANTIMICROBIANA PARA INGENIERIA TISULAR OSEAInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 CIBER-BBNKing Saud University, Arabia Saudí https://doi.org/10.13039/501100002383 RSP-2023R782Universidad Católica de Valencia San Vicente Mártir https://doi.org/10.13039/501100015082 2020-231-006UCVopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2099312026-06-13T07:49:27Z
dc.title.none.fl_str_mv In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity
title In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity
spellingShingle In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity
Cano-Vicent, Alba
Alginate
Cobalt
Toxicity
Antiviral
Antibacterial
Anticancer
INGENIERIA ELECTRICA
title_short In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity
title_full In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity
title_fullStr In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity
title_full_unstemmed In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity
title_sort In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity
dc.creator.none.fl_str_mv Cano-Vicent, Alba
Martínez-Agut, Andrea
Tuñón-Molina, Alberto
Bakshi, Hamid
Alfagih, Iman M.
Serrano-Aroca, Ángel
Tambuwala, Murtaza M.
Sabater i Serra, Roser|||0000-0002-5550-7066
author Cano-Vicent, Alba
author_facet Cano-Vicent, Alba
Martínez-Agut, Andrea
Tuñón-Molina, Alberto
Bakshi, Hamid
Alfagih, Iman M.
Serrano-Aroca, Ángel
Tambuwala, Murtaza M.
Sabater i Serra, Roser|||0000-0002-5550-7066
author_role author
author2 Martínez-Agut, Andrea
Tuñón-Molina, Alberto
Bakshi, Hamid
Alfagih, Iman M.
Serrano-Aroca, Ángel
Tambuwala, Murtaza M.
Sabater i Serra, Roser|||0000-0002-5550-7066
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Escuela Técnica Superior de Ingeniería Aeroespacial y Diseño Industrial
Departamento de Ingeniería Eléctrica
Centro de Biomateriales e Ingeniería Tisular
King Saud University
Instituto de Salud Carlos III
Agencia Estatal de Investigación
Universidad Católica de Valencia San Vicente Mártir
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Alginate
Cobalt
Toxicity
Antiviral
Antibacterial
Anticancer
INGENIERIA ELECTRICA
topic Alginate
Cobalt
Toxicity
Antiviral
Antibacterial
Anticancer
INGENIERIA ELECTRICA
description [EN] Alginate crosslinked with calcium cations is a promising hydrogel for biomedical applications as it is non-toxic, has suitable mechanical properties and is insoluble in water. Cobalt has been shown to possess antibacterial capacity against Gram-positive and Gram-negative bacteria, and has an angiogenesis effect. In this study, alginate films were crosslinked with Ca2+ and Co2+ ions to explore their biological properties in terms of antiviral capacity, antibacterial properties, anticancer activity and their toxicity. The results show that the hydrogel with a very small amount of cobalt was biocompatible in vivo using the Caenorhabditis elegans model and in vitro on human keratinocyte cells and it also exhibited antibacterial activity against the life-threatening methicillinresistant Staphylococcus aureus. Furthermore, this hydrogel showed antiviral activity against a surrogate of SARSCoV-2 and anticancer properties against melanoma and colon cancer cells, which render it a promising material for biomedical applications such as wound healing and tissue engineering. Water sorption experiments, Fourier transform infrared spectroscopy, electron microscopy with Energy Dispersive X-ray Spectrometry and degradation analysis in acid aqueous medium were performed to complete the characterization of these new materials.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/209931
url https://riunet.upv.es/handle/10251/209931
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PID2020-119333RB-I00 SOPORTES BIOFUNCIONALES CON CAPACIDAD OSTEOINDUCTORA Y ANTIMICROBIANA PARA INGENIERIA TISULAR OSEA
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 CIBER-BBN
King Saud University, Arabia Saudí https://doi.org/10.13039/501100002383 RSP-2023R782
Universidad Católica de Valencia San Vicente Mártir https://doi.org/10.13039/501100015082 2020-231-006UCV
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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