In vivo and in vitro biocompatible alginate film crosslinked with Ca2+ and Co2+ manifests antiviral, antibacterial and anticancer activity

[EN] Alginate crosslinked with calcium cations is a promising hydrogel for biomedical applications as it is non-toxic, has suitable mechanical properties and is insoluble in water. Cobalt has been shown to possess antibacterial capacity against Gram-positive and Gram-negative bacteria, and has an an...

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Detalles Bibliográficos
Autores: Cano-Vicent, Alba, Martínez-Agut, Andrea, Tuñón-Molina, Alberto, Bakshi, Hamid, Alfagih, Iman M., Serrano-Aroca, Ángel, Tambuwala, Murtaza M., Sabater i Serra, Roser|||0000-0002-5550-7066
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/209931
Acceso en línea:https://riunet.upv.es/handle/10251/209931
Access Level:acceso abierto
Palabra clave:Alginate
Cobalt
Toxicity
Antiviral
Antibacterial
Anticancer
INGENIERIA ELECTRICA
Descripción
Sumario:[EN] Alginate crosslinked with calcium cations is a promising hydrogel for biomedical applications as it is non-toxic, has suitable mechanical properties and is insoluble in water. Cobalt has been shown to possess antibacterial capacity against Gram-positive and Gram-negative bacteria, and has an angiogenesis effect. In this study, alginate films were crosslinked with Ca2+ and Co2+ ions to explore their biological properties in terms of antiviral capacity, antibacterial properties, anticancer activity and their toxicity. The results show that the hydrogel with a very small amount of cobalt was biocompatible in vivo using the Caenorhabditis elegans model and in vitro on human keratinocyte cells and it also exhibited antibacterial activity against the life-threatening methicillinresistant Staphylococcus aureus. Furthermore, this hydrogel showed antiviral activity against a surrogate of SARSCoV-2 and anticancer properties against melanoma and colon cancer cells, which render it a promising material for biomedical applications such as wound healing and tissue engineering. Water sorption experiments, Fourier transform infrared spectroscopy, electron microscopy with Energy Dispersive X-ray Spectrometry and degradation analysis in acid aqueous medium were performed to complete the characterization of these new materials.