Folate transport by prawn hepatopancreas brush-border membrane vesicles.

The transport system of folic acid (Pte-Glu) b y brush-borde r membrane vesicles (BBMV ) isolated from prawn (Penaeus japonicm) hepatopancreas , was studied by measuring the uptake of Pte-Glu . This uptake was found to have two components , intravesicular transport and membrane binding . Membrane bi...

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Authors: Blaya, J. A., García Muriana, Francisco José, Ruiz Gutiérrez, Valentina, Vázquez Cueto, Carmen María, Bolufer González, José
Format: article
Publication Date:1998
Country:España
Institution:Universidad de Sevilla (US)
Repository:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/28777
Online Access:http://hdl.handle.net/11441/28777
https://doi.org/10.1023/A:1022236616277
Access Level:Open access
Keyword:Folate transport
prawn
hepatopancreas
brush-border membrane vesicles
kinetics
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repository_id_str
spelling Folate transport by prawn hepatopancreas brush-border membrane vesicles.Blaya, J. A.García Muriana, Francisco JoséRuiz Gutiérrez, ValentinaVázquez Cueto, Carmen MaríaBolufer González, JoséFolate transportprawnhepatopancreasbrush-border membrane vesicleskineticsThe transport system of folic acid (Pte-Glu) b y brush-borde r membrane vesicles (BBMV ) isolated from prawn (Penaeus japonicm) hepatopancreas , was studied by measuring the uptake of Pte-Glu . This uptake was found to have two components , intravesicular transport and membrane binding . Membrane binding was not affected by the presence of a trans - membrane pH-gradient at a short incubation period . However , a transmembrane pH - gradient increased membrane binding at 6 0 min. The transpor to f Pte-Glu appeared to be carrier-mediated , was stimulated by an inwardly proton gradient (p H 5. 5 outside , 7. 4 inside ) and was unaffected by a sodium-gradient . The relationship between pH gradient-driven Pte-Glu uptake and medium Pte-Glu concentration followed saturating Michaelis-Menten kinetics . Eadie-Hofste e representation of the pH gradient-driven Pte-Glu uptake indicated a single transport system with a Km of 0.3 7 ^ Man d Vmax of 1.06pmol/mg protein/15s . These findings indicate that BBM V isolated from prawn hepatopancreas possesses a Pte - Glu transport system similar to that described in mammalian intestine.Portland PressFisiología1998info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/28777https://doi.org/10.1023/A:1022236616277reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBioscience Reports, 18(1), 9-17http://dx.doi.org/10.1023/A:1022236616277info:eu-repo/semantics/openAccessoai:idus.us.es:11441/287772026-06-17T12:51:07Z
dc.title.none.fl_str_mv Folate transport by prawn hepatopancreas brush-border membrane vesicles.
title Folate transport by prawn hepatopancreas brush-border membrane vesicles.
spellingShingle Folate transport by prawn hepatopancreas brush-border membrane vesicles.
Blaya, J. A.
Folate transport
prawn
hepatopancreas
brush-border membrane vesicles
kinetics
title_short Folate transport by prawn hepatopancreas brush-border membrane vesicles.
title_full Folate transport by prawn hepatopancreas brush-border membrane vesicles.
title_fullStr Folate transport by prawn hepatopancreas brush-border membrane vesicles.
title_full_unstemmed Folate transport by prawn hepatopancreas brush-border membrane vesicles.
title_sort Folate transport by prawn hepatopancreas brush-border membrane vesicles.
dc.creator.none.fl_str_mv Blaya, J. A.
García Muriana, Francisco José
Ruiz Gutiérrez, Valentina
Vázquez Cueto, Carmen María
Bolufer González, José
author Blaya, J. A.
author_facet Blaya, J. A.
García Muriana, Francisco José
Ruiz Gutiérrez, Valentina
Vázquez Cueto, Carmen María
Bolufer González, José
author_role author
author2 García Muriana, Francisco José
Ruiz Gutiérrez, Valentina
Vázquez Cueto, Carmen María
Bolufer González, José
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Fisiología
dc.subject.none.fl_str_mv Folate transport
prawn
hepatopancreas
brush-border membrane vesicles
kinetics
topic Folate transport
prawn
hepatopancreas
brush-border membrane vesicles
kinetics
description The transport system of folic acid (Pte-Glu) b y brush-borde r membrane vesicles (BBMV ) isolated from prawn (Penaeus japonicm) hepatopancreas , was studied by measuring the uptake of Pte-Glu . This uptake was found to have two components , intravesicular transport and membrane binding . Membrane binding was not affected by the presence of a trans - membrane pH-gradient at a short incubation period . However , a transmembrane pH - gradient increased membrane binding at 6 0 min. The transpor to f Pte-Glu appeared to be carrier-mediated , was stimulated by an inwardly proton gradient (p H 5. 5 outside , 7. 4 inside ) and was unaffected by a sodium-gradient . The relationship between pH gradient-driven Pte-Glu uptake and medium Pte-Glu concentration followed saturating Michaelis-Menten kinetics . Eadie-Hofste e representation of the pH gradient-driven Pte-Glu uptake indicated a single transport system with a Km of 0.3 7 ^ Man d Vmax of 1.06pmol/mg protein/15s . These findings indicate that BBM V isolated from prawn hepatopancreas possesses a Pte - Glu transport system similar to that described in mammalian intestine.
publishDate 1998
dc.date.none.fl_str_mv 1998
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/28777
https://doi.org/10.1023/A:1022236616277
url http://hdl.handle.net/11441/28777
https://doi.org/10.1023/A:1022236616277
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Bioscience Reports, 18(1), 9-17
http://dx.doi.org/10.1023/A:1022236616277
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Portland Press
publisher.none.fl_str_mv Portland Press
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411575861870592
score 15.301603

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