Folate transport by prawn hepatopancreas brush-border membrane vesicles.
The transport system of folic acid (Pte-Glu) b y brush-borde r membrane vesicles (BBMV ) isolated from prawn (Penaeus japonicm) hepatopancreas , was studied by measuring the uptake of Pte-Glu . This uptake was found to have two components , intravesicular transport and membrane binding . Membrane bi...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 1998 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:idus.us.es:11441/28777 |
| Acceso en línea: | http://hdl.handle.net/11441/28777 https://doi.org/10.1023/A:1022236616277 |
| Access Level: | acceso abierto |
| Palabra clave: | Folate transport prawn hepatopancreas brush-border membrane vesicles kinetics |
| Sumario: | The transport system of folic acid (Pte-Glu) b y brush-borde r membrane vesicles (BBMV ) isolated from prawn (Penaeus japonicm) hepatopancreas , was studied by measuring the uptake of Pte-Glu . This uptake was found to have two components , intravesicular transport and membrane binding . Membrane binding was not affected by the presence of a trans - membrane pH-gradient at a short incubation period . However , a transmembrane pH - gradient increased membrane binding at 6 0 min. The transpor to f Pte-Glu appeared to be carrier-mediated , was stimulated by an inwardly proton gradient (p H 5. 5 outside , 7. 4 inside ) and was unaffected by a sodium-gradient . The relationship between pH gradient-driven Pte-Glu uptake and medium Pte-Glu concentration followed saturating Michaelis-Menten kinetics . Eadie-Hofste e representation of the pH gradient-driven Pte-Glu uptake indicated a single transport system with a Km of 0.3 7 ^ Man d Vmax of 1.06pmol/mg protein/15s . These findings indicate that BBM V isolated from prawn hepatopancreas possesses a Pte - Glu transport system similar to that described in mammalian intestine. |
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