Fast and efficient neural conversion of human hematopoietic cells

Neurons obtained directly from human somatic cells hold great promise for disease modeling and drug screening. Available protocols rely on overexpression of transcription factors using integrative vectors and are often slow, complex, and inefficient. We report a fast and efficient approach for gener...

Full description

Bibliographic Details
Authors: Castaño, Julio, Menéndez Buján, Pablo, Bruzos-Cidon, Cristina, Straccia, Marco, Sousa, Amaia, Zabaleta, Lorea, Vazquez, Nerea, Zubiarrain, Amaia, Sonntag, Kai Christian, Ugedo, Luisa, Carvajal-Vergara, Xonia, Canals i Coll, Josep M., Torrecilla, María, Sanchez-Pernaute, Rosario, Giorgetti, Alessandra
Format: article
Status:Published version
Publication Date:2014
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/121199
Online Access:https://hdl.handle.net/2445/121199
Access Level:Open access
Keyword:Leucòcits
Fisiologia cel·lular
Xarxes neuronals (Neurobiologia)
Leucocytes
Cell physiology
Neural networks (Neurobiology)
id ES_7b53ef99ddcf41246686c992aaa23288
oai_identifier_str oai:recercat.cat:2445/121199
network_acronym_str ES
network_name_str España
repository_id_str
spelling Fast and efficient neural conversion of human hematopoietic cellsCastaño, JulioMenéndez Buján, PabloBruzos-Cidon, CristinaStraccia, MarcoSousa, AmaiaZabaleta, LoreaVazquez, NereaZubiarrain, AmaiaSonntag, Kai ChristianUgedo, LuisaCarvajal-Vergara, XoniaCanals i Coll, Josep M.Torrecilla, MaríaSanchez-Pernaute, RosarioGiorgetti, AlessandraLeucòcitsFisiologia cel·lularXarxes neuronals (Neurobiologia)LeucocytesCell physiologyNeural networks (Neurobiology)Neurons obtained directly from human somatic cells hold great promise for disease modeling and drug screening. Available protocols rely on overexpression of transcription factors using integrative vectors and are often slow, complex, and inefficient. We report a fast and efficient approach for generating induced neural cells (iNCs) directly from human hematopoietic cells using Sendai virus. Upon SOX2 and c-MYC expression, CD133-positive cord blood cells rapidly adopt a neuroepithelial morphology and exhibit high expansion capacity. Under defined neurogenic culture conditions, they express mature neuronal markers and fire spontaneous action potentials that can be modulated with neurotransmitters. SOX2 and c-MYC are also sufficient to convert peripheral blood mononuclear cells into iNCs. However, the conversion process is less efficient and resulting iNCs have limited expansion capacity and electrophysiological activity upon differentiation. Our study demonstrates rapid and efficient generation of iNCs from hematopoietic cells while underscoring the impact of target cells on conversion efficiency.Elsevier2018201820142018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion14 p.application/pdfhttps://hdl.handle.net/2445/121199Articles publicats en revistes (Biomedicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1016/j.stemcr.2014.10.008Stem Cell Reports, 2014, vol. 3, num. 6, p. 1118-1131https://doi.org/10.1016/j.stemcr.2014.10.008cc-by (c) Castaño, Julio et al., 2014http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/1211992026-05-29T05:05:01Z
dc.title.none.fl_str_mv Fast and efficient neural conversion of human hematopoietic cells
title Fast and efficient neural conversion of human hematopoietic cells
spellingShingle Fast and efficient neural conversion of human hematopoietic cells
Castaño, Julio
Leucòcits
Fisiologia cel·lular
Xarxes neuronals (Neurobiologia)
Leucocytes
Cell physiology
Neural networks (Neurobiology)
title_short Fast and efficient neural conversion of human hematopoietic cells
title_full Fast and efficient neural conversion of human hematopoietic cells
title_fullStr Fast and efficient neural conversion of human hematopoietic cells
title_full_unstemmed Fast and efficient neural conversion of human hematopoietic cells
title_sort Fast and efficient neural conversion of human hematopoietic cells
dc.creator.none.fl_str_mv Castaño, Julio
Menéndez Buján, Pablo
Bruzos-Cidon, Cristina
Straccia, Marco
Sousa, Amaia
Zabaleta, Lorea
Vazquez, Nerea
Zubiarrain, Amaia
Sonntag, Kai Christian
Ugedo, Luisa
Carvajal-Vergara, Xonia
Canals i Coll, Josep M.
Torrecilla, María
Sanchez-Pernaute, Rosario
Giorgetti, Alessandra
author Castaño, Julio
author_facet Castaño, Julio
Menéndez Buján, Pablo
Bruzos-Cidon, Cristina
Straccia, Marco
Sousa, Amaia
Zabaleta, Lorea
Vazquez, Nerea
Zubiarrain, Amaia
Sonntag, Kai Christian
Ugedo, Luisa
Carvajal-Vergara, Xonia
Canals i Coll, Josep M.
Torrecilla, María
Sanchez-Pernaute, Rosario
Giorgetti, Alessandra
author_role author
author2 Menéndez Buján, Pablo
Bruzos-Cidon, Cristina
Straccia, Marco
Sousa, Amaia
Zabaleta, Lorea
Vazquez, Nerea
Zubiarrain, Amaia
Sonntag, Kai Christian
Ugedo, Luisa
Carvajal-Vergara, Xonia
Canals i Coll, Josep M.
Torrecilla, María
Sanchez-Pernaute, Rosario
Giorgetti, Alessandra
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Leucòcits
Fisiologia cel·lular
Xarxes neuronals (Neurobiologia)
Leucocytes
Cell physiology
Neural networks (Neurobiology)
topic Leucòcits
Fisiologia cel·lular
Xarxes neuronals (Neurobiologia)
Leucocytes
Cell physiology
Neural networks (Neurobiology)
description Neurons obtained directly from human somatic cells hold great promise for disease modeling and drug screening. Available protocols rely on overexpression of transcription factors using integrative vectors and are often slow, complex, and inefficient. We report a fast and efficient approach for generating induced neural cells (iNCs) directly from human hematopoietic cells using Sendai virus. Upon SOX2 and c-MYC expression, CD133-positive cord blood cells rapidly adopt a neuroepithelial morphology and exhibit high expansion capacity. Under defined neurogenic culture conditions, they express mature neuronal markers and fire spontaneous action potentials that can be modulated with neurotransmitters. SOX2 and c-MYC are also sufficient to convert peripheral blood mononuclear cells into iNCs. However, the conversion process is less efficient and resulting iNCs have limited expansion capacity and electrophysiological activity upon differentiation. Our study demonstrates rapid and efficient generation of iNCs from hematopoietic cells while underscoring the impact of target cells on conversion efficiency.
publishDate 2014
dc.date.none.fl_str_mv 2014
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/121199
url https://hdl.handle.net/2445/121199
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.stemcr.2014.10.008
Stem Cell Reports, 2014, vol. 3, num. 6, p. 1118-1131
https://doi.org/10.1016/j.stemcr.2014.10.008
dc.rights.none.fl_str_mv cc-by (c) Castaño, Julio et al., 2014
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Castaño, Julio et al., 2014
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 14 p.
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Articles publicats en revistes (Biomedicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411506772246528
score 15,812429

Similar Items