Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System

Pomegranate (Punica granatum L.) is a common edible fruit. Its juice can be used as a source of antioxidative compounds, primarily polyphenols and vitamin C, in addition to other vitamins and minerals. Nevertheless, little is still known about how the enzymatic machinery, mainly that related to oxid...

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Authors: Pinilla, Melisa, Iglesias-Moya, Jessica, Campos, María Jesús, Corpas, Francisco J., Palma Martínez, José Manuel
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/191528
Online Access:http://hdl.handle.net/10261/191528
Access Level:Open access
Keyword:Juice
SDS-PAGE
Peroxisomes
Seeds
Superoxide dismutase
Catalase
Glyoxylate cycle
Varieties Valenciana and Mollar
Isozymes
NADP-dehydrogenases
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spelling Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating SystemPinilla, MelisaIglesias-Moya, JessicaCampos, María JesúsCorpas, Francisco J.Palma Martínez, José ManuelJuiceSDS-PAGEPeroxisomesSeedsSuperoxide dismutaseCatalaseGlyoxylate cycleVarieties Valenciana and MollarIsozymesNADP-dehydrogenasesPomegranate (Punica granatum L.) is a common edible fruit. Its juice can be used as a source of antioxidative compounds, primarily polyphenols and vitamin C, in addition to other vitamins and minerals. Nevertheless, little is still known about how the enzymatic machinery, mainly that related to oxidative metabolism, is influenced by the genotype and the environmental and climate conditions where pomegranate plants grow. In this work, seeds and juices from two pomegranate varieties (Valenciana and Mollar) grown in two different Spanish locations were assayed. Both varieties showed clear differences in their respective polypeptide profiles. The analysis of the isoenzymatic superoxide dismutase (SOD) activity pattern displayed oneMn-SODand five CuZn-SODs (I-V) whose abundances depended on the variety. Furthermore, by immunoblot assays, at least one additional Fe-SOD with a subunit size of about 23 kDa was also detected in both varieties. Besides this, the presence of the H2O2-scavenging peroxisomal catalase in seeds and juice indicates that an active metabolism of reactive oxygen species (ROS) takes place in this fruit, but the two pomegranate varieties showed opposite activity profiles. The activities of the main NADPH-regenerating enzymes, including glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphlogluconate dehydrogenase (6PGDH), NADP-dependent isocitrate dehydrogenase (NADP-ICDH), and NADP-dependent malic enzyme (NADP-ME), were studied in the same plant materials, and they behaved differently depending on the genotype. Finally, our data demonstrate the presence of two specific enzymes of the peroxisomal glyoxylate cycle, malate synthase (MS) and isocitrate lyase (ICL). These enzymes participate in oilseeds by channeling the lipid catabolism to the carbohydrate synthesis for further use in seed germination and early seedling growth. The results obtained in this work indicate that a similar mechanism to that reported in oilseeds may also operate in pomegranate.This research was achieved by an ERDF-cofinanced grant from the Ministry of Economy and Competitiveness (AGL2015-65104-P) and the Junta de Andalucía (group BIO192), Spain.Peer ReviewedMolecular Diversity Preservation InternationalMinisterio de Economía y Competitividad (España)Junta de AndalucíaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/191528reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-65104-Phttps://www.mdpi.com/2073-4395/9/6/338Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1915282026-05-22T06:33:51Z
dc.title.none.fl_str_mv Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System
title Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System
spellingShingle Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System
Pinilla, Melisa
Juice
SDS-PAGE
Peroxisomes
Seeds
Superoxide dismutase
Catalase
Glyoxylate cycle
Varieties Valenciana and Mollar
Isozymes
NADP-dehydrogenases
title_short Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System
title_full Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System
title_fullStr Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System
title_full_unstemmed Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System
title_sort Pomegranate (Punica granatum L.) Fruits: Characterization of the main enzymatic antioxidants (Peroxisomal Catalase and SOD Isozymes) and the NADPH-Regenerating System
dc.creator.none.fl_str_mv Pinilla, Melisa
Iglesias-Moya, Jessica
Campos, María Jesús
Corpas, Francisco J.
Palma Martínez, José Manuel
author Pinilla, Melisa
author_facet Pinilla, Melisa
Iglesias-Moya, Jessica
Campos, María Jesús
Corpas, Francisco J.
Palma Martínez, José Manuel
author_role author
author2 Iglesias-Moya, Jessica
Campos, María Jesús
Corpas, Francisco J.
Palma Martínez, José Manuel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Junta de Andalucía
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Juice
SDS-PAGE
Peroxisomes
Seeds
Superoxide dismutase
Catalase
Glyoxylate cycle
Varieties Valenciana and Mollar
Isozymes
NADP-dehydrogenases
topic Juice
SDS-PAGE
Peroxisomes
Seeds
Superoxide dismutase
Catalase
Glyoxylate cycle
Varieties Valenciana and Mollar
Isozymes
NADP-dehydrogenases
description Pomegranate (Punica granatum L.) is a common edible fruit. Its juice can be used as a source of antioxidative compounds, primarily polyphenols and vitamin C, in addition to other vitamins and minerals. Nevertheless, little is still known about how the enzymatic machinery, mainly that related to oxidative metabolism, is influenced by the genotype and the environmental and climate conditions where pomegranate plants grow. In this work, seeds and juices from two pomegranate varieties (Valenciana and Mollar) grown in two different Spanish locations were assayed. Both varieties showed clear differences in their respective polypeptide profiles. The analysis of the isoenzymatic superoxide dismutase (SOD) activity pattern displayed oneMn-SODand five CuZn-SODs (I-V) whose abundances depended on the variety. Furthermore, by immunoblot assays, at least one additional Fe-SOD with a subunit size of about 23 kDa was also detected in both varieties. Besides this, the presence of the H2O2-scavenging peroxisomal catalase in seeds and juice indicates that an active metabolism of reactive oxygen species (ROS) takes place in this fruit, but the two pomegranate varieties showed opposite activity profiles. The activities of the main NADPH-regenerating enzymes, including glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphlogluconate dehydrogenase (6PGDH), NADP-dependent isocitrate dehydrogenase (NADP-ICDH), and NADP-dependent malic enzyme (NADP-ME), were studied in the same plant materials, and they behaved differently depending on the genotype. Finally, our data demonstrate the presence of two specific enzymes of the peroxisomal glyoxylate cycle, malate synthase (MS) and isocitrate lyase (ICL). These enzymes participate in oilseeds by channeling the lipid catabolism to the carbohydrate synthesis for further use in seed germination and early seedling growth. The results obtained in this work indicate that a similar mechanism to that reported in oilseeds may also operate in pomegranate.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/191528
url http://hdl.handle.net/10261/191528
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-65104-P
https://www.mdpi.com/2073-4395/9/6/338
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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