Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation

Infection is the leading cause of non-relapse-related mortality after allogeneic haematopoietic stem cell transplantation (HSCT). Altered functions of immune cells in nasal secretions may influence post HSCT susceptibility to viral respiratory infections. In this prospective study, we determined T a...

Descripción completa

Detalles Bibliográficos
Autores: Vela, María, Rosal, Teresa del, Pérez Martínez, Antonio, Valentín, Jaime, Casas, Inmaculada, Pozo, Francisco, Reinoso Barbero, Francisco, Bueno, David, Corral, Dolores, Méndez Echevarría, Ana María, Mozo, Yasmina, Calvo Rey, Cristina
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/691520
Acceso en línea:http://hdl.handle.net/10486/691520
https://dx.doi.org/10.1038/s41598-019-55398-y
Access Level:acceso abierto
Palabra clave:Mucosal NK cells
Infection
Cell transplantation (HSCT)
Children
Haematopoietic stem
Medicina
id ES_7af08226e1bedfb75c19c808f2efaed0
oai_identifier_str oai:repositorio.uam.es:10486/691520
network_acronym_str ES
network_name_str España
repository_id_str
spelling Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantationVela, MaríaRosal, Teresa delPérez Martínez, AntonioValentín, JaimeCasas, InmaculadaPozo, FranciscoReinoso Barbero, FranciscoBueno, DavidCorral, DoloresMéndez Echevarría, Ana MaríaMozo, YasminaCalvo Rey, CristinaMucosal NK cellsInfectionCell transplantation (HSCT)ChildrenHaematopoietic stemMedicinaInfection is the leading cause of non-relapse-related mortality after allogeneic haematopoietic stem cell transplantation (HSCT). Altered functions of immune cells in nasal secretions may influence post HSCT susceptibility to viral respiratory infections. In this prospective study, we determined T and NK cell numbers together with NK activation status in nasopharyngeal aspirates (NPA) in HSCT recipients and healthy controls using multiparametric flow cytometry. We also determined by polymerase chain reaction (PCR) the presence of 16 respiratory viruses. Samples were collected pre-HSCT, at day 0, +10, +20 and +30 after HSCT. Peripheral blood (PB) was also analyzed to determine T and NK cell numbers. A total of 27 pediatric HSCT recipients were enrolled and 16 of them had at least one viral detection (60%). Rhinovirus was the most frequent pathogen (84% of positive NPAs). NPAs of patients contained fewer T and NK cells compared to healthy controls (p = 0.0132 and p = 0.120, respectively). Viral PCR + patients showed higher NK cell number in their NPAs. The activating receptors repertoire expressed by NK cells was also higher in NPA samples, especially NKp44 and NKp46. Our study supports NK cells relevance for the immune defense against respiratory viruses in HSCT recipientsThis work was supported in part by the National Health Service of Spain, Instituto de Salud Carlos III (ISCIII), FONDOS FEDER grant (FIS) PI18/01301, CRIS Foundation to Beat Cancer, Patients’ Support Associations Fundación Mari Paz Jiménez Casado and La Sonrisa de Álex and a Small Grant Award from the European Society for Paediatric Infectious DiseasesNature Research (part of Springer Nature)Departamento de Anatomía, Histología y NeurocienciaDepartamento de PediatríaFacultad de Medicina20192019-01-01research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/691520https://dx.doi.org/10.1038/s41598-019-55398-yreponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6915202026-06-23T12:46:27Z
dc.title.none.fl_str_mv Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
spellingShingle Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
Vela, María
Mucosal NK cells
Infection
Cell transplantation (HSCT)
Children
Haematopoietic stem
Medicina
title_short Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title_full Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title_fullStr Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title_full_unstemmed Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
title_sort Possible role of highly activated mucosal NK cells against viral respiratory infections in children undergoing haematopoietic stem cell transplantation
dc.creator.none.fl_str_mv Vela, María
Rosal, Teresa del
Pérez Martínez, Antonio
Valentín, Jaime
Casas, Inmaculada
Pozo, Francisco
Reinoso Barbero, Francisco
Bueno, David
Corral, Dolores
Méndez Echevarría, Ana María
Mozo, Yasmina
Calvo Rey, Cristina
author Vela, María
author_facet Vela, María
Rosal, Teresa del
Pérez Martínez, Antonio
Valentín, Jaime
Casas, Inmaculada
Pozo, Francisco
Reinoso Barbero, Francisco
Bueno, David
Corral, Dolores
Méndez Echevarría, Ana María
Mozo, Yasmina
Calvo Rey, Cristina
author_role author
author2 Rosal, Teresa del
Pérez Martínez, Antonio
Valentín, Jaime
Casas, Inmaculada
Pozo, Francisco
Reinoso Barbero, Francisco
Bueno, David
Corral, Dolores
Méndez Echevarría, Ana María
Mozo, Yasmina
Calvo Rey, Cristina
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Anatomía, Histología y Neurociencia
Departamento de Pediatría
Facultad de Medicina
dc.subject.none.fl_str_mv Mucosal NK cells
Infection
Cell transplantation (HSCT)
Children
Haematopoietic stem
Medicina
topic Mucosal NK cells
Infection
Cell transplantation (HSCT)
Children
Haematopoietic stem
Medicina
description Infection is the leading cause of non-relapse-related mortality after allogeneic haematopoietic stem cell transplantation (HSCT). Altered functions of immune cells in nasal secretions may influence post HSCT susceptibility to viral respiratory infections. In this prospective study, we determined T and NK cell numbers together with NK activation status in nasopharyngeal aspirates (NPA) in HSCT recipients and healthy controls using multiparametric flow cytometry. We also determined by polymerase chain reaction (PCR) the presence of 16 respiratory viruses. Samples were collected pre-HSCT, at day 0, +10, +20 and +30 after HSCT. Peripheral blood (PB) was also analyzed to determine T and NK cell numbers. A total of 27 pediatric HSCT recipients were enrolled and 16 of them had at least one viral detection (60%). Rhinovirus was the most frequent pathogen (84% of positive NPAs). NPAs of patients contained fewer T and NK cells compared to healthy controls (p = 0.0132 and p = 0.120, respectively). Viral PCR + patients showed higher NK cell number in their NPAs. The activating receptors repertoire expressed by NK cells was also higher in NPA samples, especially NKp44 and NKp46. Our study supports NK cells relevance for the immune defense against respiratory viruses in HSCT recipients
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/691520
https://dx.doi.org/10.1038/s41598-019-55398-y
url http://hdl.handle.net/10486/691520
https://dx.doi.org/10.1038/s41598-019-55398-y
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research (part of Springer Nature)
publisher.none.fl_str_mv Nature Research (part of Springer Nature)
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411477329281024
score 15,300719

Similares en LA Referencia