In vivo expansion of a CD9+ decidual-like NK cell subset following autologous hematopoietic stem cell transplantation

Autologous hematopoietic stem cell transplantation (autoHSCT) is a treatment option for hematological disorders and pediatric solid tumors. After an autoHSCT, natural killer (NK) cells are the first lymphocyte subset returning to normal levels. To uncover global changes during NK cell reconstitution...

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Detalles Bibliográficos
Autores: Orrantia Robles, Ane, Vázquez de Luís, Enrique, Astarloa Pando, Gabirel, Terrén Martínez, Iñigo, Amarilla Irusta, Ainhoa, Polanco Alonso, Diego, González, Carmen, Uranga, Alasne, Carrascosa, Tomás, Mateos Mazón, Juán José, García Ruiz, Juan Carlos, Callejas, Sergio, Quintas, Ana, Dopazo, Ana, Zenarruzabeitia Belaustegui, Olatz, Borrego Rabasco, Francisco
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/69452
Acceso en línea:http://hdl.handle.net/10810/69452
Access Level:acceso abierto
Palabra clave:NK cells
decidual NK cells
autologous hematopoietic stem cell 48 transplantation
RNA-seq
mevalonate
autoHSCT
decidual-like
CD9
Descripción
Sumario:Autologous hematopoietic stem cell transplantation (autoHSCT) is a treatment option for hematological disorders and pediatric solid tumors. After an autoHSCT, natural killer (NK) cells are the first lymphocyte subset returning to normal levels. To uncover global changes during NK cell reconstitution after autoHSCT, we performed RNA-sequencing on NK cells before and after autoHSCT. Results showed profound changes in gene expression profile of NK cells immediately after autoHSCT. Several biological processes including cell cycle, DNA replication and the mevalonate pathway were enriched. Significantly, we observed that following autoHSCT, NK cells acquired a decidual-like gene expression profile, including the expression of CD9. By using multiparametric flow cytometry, we confirmed the expansion of NK cells expressing CD9 immediately after autoHSCT, which exhibited higher granzyme B and perforin expression levels than CD9- NK cells. These results provide insights on the physiopathology of NK cells during their reconstitution after autoHSCT.

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