Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of...
| Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
| Repositorio: | r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
| OAI Identifier: | oai:iibsantpau.fundanetsuite.com:p7692 |
| Acceso en línea: | https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=7692 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123571981&doi=10.1038%2fs41598-022-05315-7&partnerID=40&md5=db1679cd167d201b2d9278e8a789ecad |
| Access Level: | acceso abierto |
| Palabra clave: | atypical Mycobacterium classification clinical trial human isolation and purification matrix-assisted laser desorption-ionization mass spectrometry multicenter study reproducibility species difference Humans Nontuberculous Mycobacteria Reproducibility of Results Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
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Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identificationRodriguez-Temporal, DAlcaide, FMarekovic, IO'Connor, JAGorton, Rvan Ingen, JVan den Bossche, AHery-Arnaud, GBeauruelle, COrth-Holler, DPalacios-Gutierrez, JJTudo, GBou, GCeyssens, PJGarrigo, MGonzalez-Martin, JGreub, GHrabak, JIngebretsen, AMediavilla-Gradolph, MCOviano, MPalop, BPranada, ABQuiroga, LRuiz-Serrano, MJRodriguez-Sanchez, Batypical Mycobacteriumclassificationclinical trialhumanisolation and purificationmatrix-assisted laser desorption-ionization mass spectrometrymulticenter studyreproducibilityspecies differenceHumansNontuberculous MycobacteriaReproducibility of ResultsSpecies SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationThe ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.NATURE RESEARCH2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=7692https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123571981&doi=10.1038%2fs41598-022-05315-7&partnerID=40&md5=db1679cd167d201b2d9278e8a789ecadScientific ReportsISSN: 20452322reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p76922026-06-14T12:41:47Z |
| dc.title.none.fl_str_mv |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
| title |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
| spellingShingle |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification Rodriguez-Temporal, D atypical Mycobacterium classification clinical trial human isolation and purification matrix-assisted laser desorption-ionization mass spectrometry multicenter study reproducibility species difference Humans Nontuberculous Mycobacteria Reproducibility of Results Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| title_short |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
| title_full |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
| title_fullStr |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
| title_full_unstemmed |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
| title_sort |
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification |
| dc.creator.none.fl_str_mv |
Rodriguez-Temporal, D Alcaide, F Marekovic, I O'Connor, JA Gorton, R van Ingen, J Van den Bossche, A Hery-Arnaud, G Beauruelle, C Orth-Holler, D Palacios-Gutierrez, JJ Tudo, G Bou, G Ceyssens, PJ Garrigo, M Gonzalez-Martin, J Greub, G Hrabak, J Ingebretsen, A Mediavilla-Gradolph, MC Oviano, M Palop, B Pranada, AB Quiroga, L Ruiz-Serrano, MJ Rodriguez-Sanchez, B |
| author |
Rodriguez-Temporal, D |
| author_facet |
Rodriguez-Temporal, D Alcaide, F Marekovic, I O'Connor, JA Gorton, R van Ingen, J Van den Bossche, A Hery-Arnaud, G Beauruelle, C Orth-Holler, D Palacios-Gutierrez, JJ Tudo, G Bou, G Ceyssens, PJ Garrigo, M Gonzalez-Martin, J Greub, G Hrabak, J Ingebretsen, A Mediavilla-Gradolph, MC Oviano, M Palop, B Pranada, AB Quiroga, L Ruiz-Serrano, MJ Rodriguez-Sanchez, B |
| author_role |
author |
| author2 |
Alcaide, F Marekovic, I O'Connor, JA Gorton, R van Ingen, J Van den Bossche, A Hery-Arnaud, G Beauruelle, C Orth-Holler, D Palacios-Gutierrez, JJ Tudo, G Bou, G Ceyssens, PJ Garrigo, M Gonzalez-Martin, J Greub, G Hrabak, J Ingebretsen, A Mediavilla-Gradolph, MC Oviano, M Palop, B Pranada, AB Quiroga, L Ruiz-Serrano, MJ Rodriguez-Sanchez, B |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
atypical Mycobacterium classification clinical trial human isolation and purification matrix-assisted laser desorption-ionization mass spectrometry multicenter study reproducibility species difference Humans Nontuberculous Mycobacteria Reproducibility of Results Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| topic |
atypical Mycobacterium classification clinical trial human isolation and purification matrix-assisted laser desorption-ionization mass spectrometry multicenter study reproducibility species difference Humans Nontuberculous Mycobacteria Reproducibility of Results Species Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| description |
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Lowenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score >= 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=7692 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123571981&doi=10.1038%2fs41598-022-05315-7&partnerID=40&md5=db1679cd167d201b2d9278e8a789ecad |
| url |
https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=7692 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85123571981&doi=10.1038%2fs41598-022-05315-7&partnerID=40&md5=db1679cd167d201b2d9278e8a789ecad |
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Inglés |
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Inglés |
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info:eu-repo/semantics/openAccess |
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openAccess |
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NATURE RESEARCH |
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NATURE RESEARCH |
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Scientific Reports ISSN: 20452322 reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
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Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau) |
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r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
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r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau |
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