Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on Biocompatibility

Mixtures of two cationic proteins were used to prepare protein-DNA gel particles, employing associative phase separation and interfacial diffusion (Morán et al., 2009a). By mixing the two proteins, we have obtained particles that displayed higher loading efficiency and loading capacity values than t...

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Detalhes bibliográficos
Autores: Morán Badenas, María del Carmen, Nogueira, Daniele R., Vinardell Martínez-Hidalgo, Ma. Pilar, Miguel, M. Graça, Lindman, Björn, 1942-
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2013
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/127935
Acesso em linha:https://hdl.handle.net/2445/127935
Access Level:acceso abierto
Palavra-chave:Gels (Farmàcia)
ADN
Partícules (Matèria)
Mort cel·lular
Càncer
Gels (Pharmacy)
DNA
Particles
Cell death
Cancer
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spelling Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on BiocompatibilityMorán Badenas, María del CarmenNogueira, Daniele R.Vinardell Martínez-Hidalgo, Ma. PilarMiguel, M. GraçaLindman, Björn, 1942-Gels (Farmàcia)ADNPartícules (Matèria)Mort cel·lularCàncerGels (Pharmacy)DNAParticlesCell deathCancerMixtures of two cationic proteins were used to prepare protein-DNA gel particles, employing associative phase separation and interfacial diffusion (Morán et al., 2009a). By mixing the two proteins, we have obtained particles that displayed higher loading efficiency and loading capacity values than those obtained in single-protein systems. However, nothing is known about the adverse effects on haemocompatibility and cytotoxicity of these protein-DNA gel particles. Here, we examined the interaction of protein-DNA gel particles obtained by two different preparation methods, and their components, with red blood cells and established cells. From a haemolytic point of view, these protein-DNA gel particles were demonstrated to be promising long-term blood-contacting medical devices. Safety evaluation with the established cell lines revealed that, in comparison with proteins in solution, the cytotoxicity was reduced when administered in the protein-DNA systems. In comparison with large-sized particles, the cytotoxic responses of small-sized protein-DNA gel particles showed to be strongly dependent of both the protein composition and the cell line being the tumour cell line HeLa more sensitive to the deleterious effects of the mixed protein-based particles. The observed trends in haemolysis and cell viabilities were in agreement with the degree of complexation values obtained for the protein-DNA gel particles prepared by both preparation methods.Elsevier B.V.2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/127935Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.ijpharm.2013.06.041International Journal of Pharmaceutics, 2013, vol. 454, num. 1, p. 192-203https://doi.org/10.1016/j.ijpharm.2013.06.041(c) Elsevier B.V., 2013info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1279352026-05-27T06:46:51Z
dc.title.none.fl_str_mv Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on Biocompatibility
title Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on Biocompatibility
spellingShingle Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on Biocompatibility
Morán Badenas, María del Carmen
Gels (Farmàcia)
ADN
Partícules (Matèria)
Mort cel·lular
Càncer
Gels (Pharmacy)
DNA
Particles
Cell death
Cancer
title_short Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on Biocompatibility
title_full Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on Biocompatibility
title_fullStr Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on Biocompatibility
title_full_unstemmed Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on Biocompatibility
title_sort Mixed Protein-DNA Gel Particles for DNA Delivery: Role of Protein Composition and Preparation Method on Biocompatibility
dc.creator.none.fl_str_mv Morán Badenas, María del Carmen
Nogueira, Daniele R.
Vinardell Martínez-Hidalgo, Ma. Pilar
Miguel, M. Graça
Lindman, Björn, 1942-
author Morán Badenas, María del Carmen
author_facet Morán Badenas, María del Carmen
Nogueira, Daniele R.
Vinardell Martínez-Hidalgo, Ma. Pilar
Miguel, M. Graça
Lindman, Björn, 1942-
author_role author
author2 Nogueira, Daniele R.
Vinardell Martínez-Hidalgo, Ma. Pilar
Miguel, M. Graça
Lindman, Björn, 1942-
author2_role author
author
author
author
dc.subject.none.fl_str_mv Gels (Farmàcia)
ADN
Partícules (Matèria)
Mort cel·lular
Càncer
Gels (Pharmacy)
DNA
Particles
Cell death
Cancer
topic Gels (Farmàcia)
ADN
Partícules (Matèria)
Mort cel·lular
Càncer
Gels (Pharmacy)
DNA
Particles
Cell death
Cancer
description Mixtures of two cationic proteins were used to prepare protein-DNA gel particles, employing associative phase separation and interfacial diffusion (Morán et al., 2009a). By mixing the two proteins, we have obtained particles that displayed higher loading efficiency and loading capacity values than those obtained in single-protein systems. However, nothing is known about the adverse effects on haemocompatibility and cytotoxicity of these protein-DNA gel particles. Here, we examined the interaction of protein-DNA gel particles obtained by two different preparation methods, and their components, with red blood cells and established cells. From a haemolytic point of view, these protein-DNA gel particles were demonstrated to be promising long-term blood-contacting medical devices. Safety evaluation with the established cell lines revealed that, in comparison with proteins in solution, the cytotoxicity was reduced when administered in the protein-DNA systems. In comparison with large-sized particles, the cytotoxic responses of small-sized protein-DNA gel particles showed to be strongly dependent of both the protein composition and the cell line being the tumour cell line HeLa more sensitive to the deleterious effects of the mixed protein-based particles. The observed trends in haemolysis and cell viabilities were in agreement with the degree of complexation values obtained for the protein-DNA gel particles prepared by both preparation methods.
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/127935
url https://hdl.handle.net/2445/127935
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.ijpharm.2013.06.041
International Journal of Pharmaceutics, 2013, vol. 454, num. 1, p. 192-203
https://doi.org/10.1016/j.ijpharm.2013.06.041
dc.rights.none.fl_str_mv (c) Elsevier B.V., 2013
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Elsevier B.V., 2013
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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