Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signatures

In recent years, the utility of lipidomics has been recognized in environmental toxicology and biomonitoring efforts due to the ubiquitous nature and importance of lipids in many cellular processes including signal transduction, energy storage, and cellular compartmentalization. Additionally, techno...

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Autores: Ahmadireskety, Atiye, Aristizabal-Henao, Juan J., Marqueño, Anna, Perrault, Justin R., Stacy, Nicole I.
Formato: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/221065
Acesso em linha:http://hdl.handle.net/10261/221065
Access Level:acceso abierto
Palavra-chave:Lipidomics
UHPLC–HRMS/MS
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spelling Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signaturesAhmadireskety, AtiyeAristizabal-Henao, Juan J.Marqueño, AnnaPerrault, Justin R.Stacy, Nicole I.LipidomicsUHPLC–HRMS/MSIn recent years, the utility of lipidomics has been recognized in environmental toxicology and biomonitoring efforts due to the ubiquitous nature and importance of lipids in many cellular processes including signal transduction, energy storage, and cellular compartmentalization. Additionally, technological advances in high-resolution mass spectrometry have enabled the rapid expansion of the field, creating a surge in interest in comparative studies of lipid metabolism from a Systems Biology standpoint. Here, we adapted a nontargeted lipidomic approach for the study of plasma samples from nesting female leatherback (Dermochelys coriacea), loggerhead (Caretta caretta), and green (Chelonia mydas) sea turtles in Florida using ultra-high-performance liquid chromatography/high-resolution tandem mass spectrometry. We identified 877 lipids in common between the three species, of which the concentrations for 467 lipids were statistically different between two or more group comparisons. Principal component analysis revealed unique lipidomic signatures associated with each species of turtle, including various glycerophosphatidylcholines, glycerophosphatidylethanolamines, triacylglycerols, and oxidized triacylglycerols that were higher in leatherback sea turtles, diacylglycerols and select glycerophosphatidylinositols which were higher in loggerhead sea turtles, and specific plasmanyl-phosphatidylcholines that were higher in green sea turtles. Our results indicate that lipidomic profiling can be a useful tool for studying lipid metabolism and physiology of different species of sea turtles, while establishing baseline data that may be used as reference in future studies for observation of differences in life stages, for following spatial and temporal trends in nesting turtles, and for investigating population dynamics in response to various stressors.Partial funding for sample collection and nightly surveys was provided by The Albert E. and Birdie W. Einstein Fund. The corresponding author would like to acknowledge funding support from the University of Florida College of Veterinary Medicine startup package.Peer reviewedSpringer NatureConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/221065reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1007/s00227-020-03747-1Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2210652026-05-22T06:33:51Z
dc.title.none.fl_str_mv Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signatures
title Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signatures
spellingShingle Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signatures
Ahmadireskety, Atiye
Lipidomics
UHPLC–HRMS/MS
title_short Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signatures
title_full Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signatures
title_fullStr Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signatures
title_full_unstemmed Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signatures
title_sort Nontargeted lipidomics in nesting females of three sea turtle species in Florida by ultra-high-pressure liquid chromatography–high-resolution tandem mass spectrometry (UHPLC–HRMS/MS) reveals distinct species-specific lipid signatures
dc.creator.none.fl_str_mv Ahmadireskety, Atiye
Aristizabal-Henao, Juan J.
Marqueño, Anna
Perrault, Justin R.
Stacy, Nicole I.
author Ahmadireskety, Atiye
author_facet Ahmadireskety, Atiye
Aristizabal-Henao, Juan J.
Marqueño, Anna
Perrault, Justin R.
Stacy, Nicole I.
author_role author
author2 Aristizabal-Henao, Juan J.
Marqueño, Anna
Perrault, Justin R.
Stacy, Nicole I.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Lipidomics
UHPLC–HRMS/MS
topic Lipidomics
UHPLC–HRMS/MS
description In recent years, the utility of lipidomics has been recognized in environmental toxicology and biomonitoring efforts due to the ubiquitous nature and importance of lipids in many cellular processes including signal transduction, energy storage, and cellular compartmentalization. Additionally, technological advances in high-resolution mass spectrometry have enabled the rapid expansion of the field, creating a surge in interest in comparative studies of lipid metabolism from a Systems Biology standpoint. Here, we adapted a nontargeted lipidomic approach for the study of plasma samples from nesting female leatherback (Dermochelys coriacea), loggerhead (Caretta caretta), and green (Chelonia mydas) sea turtles in Florida using ultra-high-performance liquid chromatography/high-resolution tandem mass spectrometry. We identified 877 lipids in common between the three species, of which the concentrations for 467 lipids were statistically different between two or more group comparisons. Principal component analysis revealed unique lipidomic signatures associated with each species of turtle, including various glycerophosphatidylcholines, glycerophosphatidylethanolamines, triacylglycerols, and oxidized triacylglycerols that were higher in leatherback sea turtles, diacylglycerols and select glycerophosphatidylinositols which were higher in loggerhead sea turtles, and specific plasmanyl-phosphatidylcholines that were higher in green sea turtles. Our results indicate that lipidomic profiling can be a useful tool for studying lipid metabolism and physiology of different species of sea turtles, while establishing baseline data that may be used as reference in future studies for observation of differences in life stages, for following spatial and temporal trends in nesting turtles, and for investigating population dynamics in response to various stressors.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/221065
url http://hdl.handle.net/10261/221065
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1007/s00227-020-03747-1
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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