Comparative Phytochemical Profiling and Wound Healing Potential of Scabiosa pseudograminifolia Hub.-Mor. and Scabiosa hololeuca Bornm.: UHPLC-HRMS/MS Analysis and Fibroblast-Based Evaluation
Species of the genus Scabiosa have long been used in traditional medicine for the treatment of skin disorders and inflammatoryconditions; however, comparative information on their chemical composition and biological activity remains limited. In thisstudy, the phenolic composition and functional prop...
| Autores: | , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2026 |
| País: | España |
| Institución: | Universidad de Sevilla (US) |
| Repositorio: | idUS. Depósito de Investigación de la Universidad de Sevilla |
| OAI Identifier: | oai:dnet:idus________::6a7d1c67a8aeb025cfb21aefe95f4ecf |
| Acceso en línea: | https://hdl.handle.net/11441/186281 https://doi.org/10.1002/fsn3.71738 |
| Access Level: | acceso abierto |
| Palabra clave: | Fibroblast cells Lomelosia hololeuca Lomelosia pseudograminifolia UHPLC-HRMS/MS wound healing |
| Sumario: | Species of the genus Scabiosa have long been used in traditional medicine for the treatment of skin disorders and inflammatoryconditions; however, comparative information on their chemical composition and biological activity remains limited. In thisstudy, the phenolic composition and functional properties of Scabiosa pseudograminifolia Hub.-Mor. and Scabiosa hololeucaBornm. were investigated using microwave-assisted extraction combined with UHPLC-HRMS/MS analysis and in vitro biologi-cal assays. Methanolic and aqueous extracts were analyzed for their phenolic constituents and evaluated in NIH/3T3 fibroblastsfor intracellular reactive oxygen species (ROS) modulation, cell viability, and wound healing activity. Antimicrobial activity wasadditionally assessed using a broth microdilution method against selected bacterial and fungal strains. All extracts significantlyreduced tert-butyl hydroperoxide (tBHP)-induced intracellular ROS levels, demonstrating antioxidant activity in the cellularmodel. Extracts of S. pseudograminifolia showed a pronounced wound healing effect, promoting more than 95% wound closureafter 48 h at low concentrations, whereas those of S. hololeuca exhibited more moderate activity. The concentrations responsiblefor these effects were confirmed to be non-cytotoxic. Distinct species- and solvent-dependent differences in phenolic compositionwere observed. In S. pseudograminifolia, chlorogenic acid was identified as the major compound in both methanolic and aqueousextracts, while the aqueous extract also contained high levels of 4- O-caffeoylquinic acid. In contrast, S. hololeuca extracts con-tained lower amounts of chlorogenic acid and were characterized by relatively higher levels of caffeic and protocatechuic acids.Antimicrobial activity was weak to moderate, suggesting that the principal biological relevance of these extracts is associatedwith oxidative stress modulation and wound repair rather than direct antimicrobial effects. This study represents the first inte-grated phytochemical and biological evaluation of these two closely related taxa. |
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