Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F

The main objective of this work is the analysis of the structural and functional role of some amino acids in Bacteriorhodopsin.<br/>- Characterization of the motif Thr90-Pro91 in the centre of Helix C of Bacteriorhodopsin. Because Thr90 interacts with Asp115 through a hydrogen bond and with th...

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Detalles Bibliográficos
Autor: Perálvarez Marín, Alejandro
Tipo de recurso: tesis doctoral
Estado:Versión publicada
Fecha de publicación:2005
País:España
Institución:CBUC, CESCA
Repositorio:TDR. Tesis Doctorales en Red
OAI Identifier:oai:www.tdx.cat:10803/3542
Acceso en línea:http://www.tdx.cat/TDX-0502106-235503
http://hdl.handle.net/10803/3542
Access Level:acceso abierto
Palabra clave:Biofísica
Bacteriodopsina
Proteïnes transmembrana
Ciències de la Salut
577
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spelling Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and FPerálvarez Marín, AlejandroBiofísicaBacteriodopsinaProteïnes transmembranaCiències de la Salut577The main objective of this work is the analysis of the structural and functional role of some amino acids in Bacteriorhodopsin.<br/>- Characterization of the motif Thr90-Pro91 in the centre of Helix C of Bacteriorhodopsin. Because Thr90 interacts with Asp115 through a hydrogen bond and with the retinal through hydrophobic interaction, the mutants T90V and D115A were taken into account. The following objectives were settled:<br/>Design and construct the Bacteriorhodopsin mutants P91A, T90A,T90V and D115A by site directed mutagenesis.<br/>Express the mutants in the purple membrane of Halobacterium salinarum.<br/>Analyze the role of Thr90 and Pro91 in the properties of the protein and in the proton pumping function in particular through the study of the mutants.<br/>Infer a role to the motif Thr90-Pro91 in Bacteriorhodopsin.<br/>- Analyze the role of the other two Prolines located in transmembrane helices in BR, and to extend this role to other Transmembrane Proteins. The subsequent objectives were proposed:<br/>Design and construct the Bacteriorhodopsin mutants P50A and P186A by site directed mutagenesis.<br/>Express the mutants in the purple membrane of Halobacterium salinarum.<br/>Characterize the role of Pro50, and Pro186 in Bacteriorhodopsin properties and proton pumping through the analysis of the mutants.<br/>Infer a role to Helix-embedded prolines of Bacteriorhodopsin in particular and to transmembrane helices of proteins in general.Universitat Autònoma de BarcelonaQuerol Murillo, EnriquePadrós, EsteveUniversitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular2011200620052006info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://www.tdx.cat/TDX-0502106-235503http://hdl.handle.net/10803/3542TDX (Tesis Doctorals en Xarxa)reponame:TDR. Tesis Doctorales en Redinstname:CBUC, CESCAInglésADVERTIMENT. L'accés als continguts d'aquesta tesi doctoral i la seva utilització ha de respectar els drets de la persona autora. Pot ser utilitzada per a consulta o estudi personal, així com en activitats o materials d'investigació i docència en els termes establerts a l'art. 32 del Text Refós de la Llei de Propietat Intel·lectual (RDL 1/1996). Per altres utilitzacions es requereix l'autorització prèvia i expressa de la persona autora. En qualsevol cas, en la utilització dels seus continguts caldrà indicar de forma clara el nom i cognoms de la persona autora i el títol de la tesi doctoral. No s'autoritza la seva reproducció o altres formes d'explotació efectuades amb finalitats de lucre ni la seva comunicació pública des d'un lloc aliè al servei TDX. Tampoc s'autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant als continguts de la tesi com als seus resums i índexs.info:eu-repo/semantics/openAccessoai:www.tdx.cat:10803/35422026-06-14T12:46:07Z
dc.title.none.fl_str_mv Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F
title Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F
spellingShingle Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F
Perálvarez Marín, Alejandro
Biofísica
Bacteriodopsina
Proteïnes transmembrana
Ciències de la Salut
577
title_short Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F
title_full Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F
title_fullStr Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F
title_full_unstemmed Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F
title_sort Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F
dc.creator.none.fl_str_mv Perálvarez Marín, Alejandro
author Perálvarez Marín, Alejandro
author_facet Perálvarez Marín, Alejandro
author_role author
dc.contributor.none.fl_str_mv Querol Murillo, Enrique
Padrós, Esteve
Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular
dc.subject.none.fl_str_mv Biofísica
Bacteriodopsina
Proteïnes transmembrana
Ciències de la Salut
577
topic Biofísica
Bacteriodopsina
Proteïnes transmembrana
Ciències de la Salut
577
description The main objective of this work is the analysis of the structural and functional role of some amino acids in Bacteriorhodopsin.<br/>- Characterization of the motif Thr90-Pro91 in the centre of Helix C of Bacteriorhodopsin. Because Thr90 interacts with Asp115 through a hydrogen bond and with the retinal through hydrophobic interaction, the mutants T90V and D115A were taken into account. The following objectives were settled:<br/>Design and construct the Bacteriorhodopsin mutants P91A, T90A,T90V and D115A by site directed mutagenesis.<br/>Express the mutants in the purple membrane of Halobacterium salinarum.<br/>Analyze the role of Thr90 and Pro91 in the properties of the protein and in the proton pumping function in particular through the study of the mutants.<br/>Infer a role to the motif Thr90-Pro91 in Bacteriorhodopsin.<br/>- Analyze the role of the other two Prolines located in transmembrane helices in BR, and to extend this role to other Transmembrane Proteins. The subsequent objectives were proposed:<br/>Design and construct the Bacteriorhodopsin mutants P50A and P186A by site directed mutagenesis.<br/>Express the mutants in the purple membrane of Halobacterium salinarum.<br/>Characterize the role of Pro50, and Pro186 in Bacteriorhodopsin properties and proton pumping through the analysis of the mutants.<br/>Infer a role to Helix-embedded prolines of Bacteriorhodopsin in particular and to transmembrane helices of proteins in general.
publishDate 2005
dc.date.none.fl_str_mv 2005
2006
2006
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.tdx.cat/TDX-0502106-235503
http://hdl.handle.net/10803/3542
url http://www.tdx.cat/TDX-0502106-235503
http://hdl.handle.net/10803/3542
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universitat Autònoma de Barcelona
publisher.none.fl_str_mv Universitat Autònoma de Barcelona
dc.source.none.fl_str_mv TDX (Tesis Doctorals en Xarxa)
reponame:TDR. Tesis Doctorales en Red
instname:CBUC, CESCA
instname_str CBUC, CESCA
reponame_str TDR. Tesis Doctorales en Red
collection TDR. Tesis Doctorales en Red
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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