Dynamic Factors of Bacteriorhodopsin: Regulation Key Points in Helices B, C and F
The main objective of this work is the analysis of the structural and functional role of some amino acids in Bacteriorhodopsin.<br/>- Characterization of the motif Thr90-Pro91 in the centre of Helix C of Bacteriorhodopsin. Because Thr90 interacts with Asp115 through a hydrogen bond and with th...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2005 |
| País: | España |
| Institución: | CBUC, CESCA |
| Repositorio: | TDR. Tesis Doctorales en Red |
| OAI Identifier: | oai:www.tdx.cat:10803/3542 |
| Acceso en línea: | http://www.tdx.cat/TDX-0502106-235503 http://hdl.handle.net/10803/3542 |
| Access Level: | acceso abierto |
| Palabra clave: | Biofísica Bacteriodopsina Proteïnes transmembrana Ciències de la Salut 577 |
| Sumario: | The main objective of this work is the analysis of the structural and functional role of some amino acids in Bacteriorhodopsin.<br/>- Characterization of the motif Thr90-Pro91 in the centre of Helix C of Bacteriorhodopsin. Because Thr90 interacts with Asp115 through a hydrogen bond and with the retinal through hydrophobic interaction, the mutants T90V and D115A were taken into account. The following objectives were settled:<br/>Design and construct the Bacteriorhodopsin mutants P91A, T90A,T90V and D115A by site directed mutagenesis.<br/>Express the mutants in the purple membrane of Halobacterium salinarum.<br/>Analyze the role of Thr90 and Pro91 in the properties of the protein and in the proton pumping function in particular through the study of the mutants.<br/>Infer a role to the motif Thr90-Pro91 in Bacteriorhodopsin.<br/>- Analyze the role of the other two Prolines located in transmembrane helices in BR, and to extend this role to other Transmembrane Proteins. The subsequent objectives were proposed:<br/>Design and construct the Bacteriorhodopsin mutants P50A and P186A by site directed mutagenesis.<br/>Express the mutants in the purple membrane of Halobacterium salinarum.<br/>Characterize the role of Pro50, and Pro186 in Bacteriorhodopsin properties and proton pumping through the analysis of the mutants.<br/>Infer a role to Helix-embedded prolines of Bacteriorhodopsin in particular and to transmembrane helices of proteins in general. |
|---|