Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations

In conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was asso...

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Authors: Arellano, Juan B., Schröder, Wolfgang P., Sandmann, Gerhard, Chueca, Ana, Barón Ayala, Matilde
Format: article
Publication Date:1994
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/11101
Online Access:http://hdl.handle.net/10261/11101
Access Level:Open access
Keyword:Photosystem II
Nuclear contaminants
Copper
Histones
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spelling Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparationsArellano, Juan B.Schröder, Wolfgang P.Sandmann, GerhardChueca, AnaBarón Ayala, MatildePhotosystem IINuclear contaminantsCopperHistonesIn conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was associated with nuclear contaminants in the starch fraction. Among the contaminants, several histones were identified by specific antisera and by N-terminal sequencing. In order to obtain homogeneous BBY preparations of PSII a procedure is employed that involves a 10000 g centrifugation step and which eliminates non-specifically bound metals, nucleic acids and histones with the starch pellet. The resulting starch-free BBY (BBYs-), which is free of these nuclear contaminants, is an appropriate preparation for biophysical studies or for those of metal interactions with PSII.This work was supported by a grant from the Spanish DGICYT (Project PB 88-0092) and a Spanish-German Exchange Program (MEC-DAAD). J. B. Arellano was the recipient of a predoctoral fellowship from the Spanish Science and Education Ministry. Thanks are due to Junta de Andalucía and the Margit and Foike Pehrzon Foundation for financing W. P. Schroder's and J. B. Arellano's stays in Granada and Stockholm, respectively.Peer reviewedJohn Wiley & Sons200920091994info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501635245 bytesapplication/pdfhttp://hdl.handle.net/10261/11101reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://www3.interscience.wiley.com/journal/119267376/abstractinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/111012026-05-22T06:33:51Z
dc.title.none.fl_str_mv Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
title Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
spellingShingle Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
Arellano, Juan B.
Photosystem II
Nuclear contaminants
Copper
Histones
title_short Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
title_full Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
title_fullStr Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
title_full_unstemmed Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
title_sort Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
dc.creator.none.fl_str_mv Arellano, Juan B.
Schröder, Wolfgang P.
Sandmann, Gerhard
Chueca, Ana
Barón Ayala, Matilde
author Arellano, Juan B.
author_facet Arellano, Juan B.
Schröder, Wolfgang P.
Sandmann, Gerhard
Chueca, Ana
Barón Ayala, Matilde
author_role author
author2 Schröder, Wolfgang P.
Sandmann, Gerhard
Chueca, Ana
Barón Ayala, Matilde
author2_role author
author
author
author
dc.subject.none.fl_str_mv Photosystem II
Nuclear contaminants
Copper
Histones
topic Photosystem II
Nuclear contaminants
Copper
Histones
description In conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was associated with nuclear contaminants in the starch fraction. Among the contaminants, several histones were identified by specific antisera and by N-terminal sequencing. In order to obtain homogeneous BBY preparations of PSII a procedure is employed that involves a 10000 g centrifugation step and which eliminates non-specifically bound metals, nucleic acids and histones with the starch pellet. The resulting starch-free BBY (BBYs-), which is free of these nuclear contaminants, is an appropriate preparation for biophysical studies or for those of metal interactions with PSII.
publishDate 1994
dc.date.none.fl_str_mv 1994
2009
2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/11101
url http://hdl.handle.net/10261/11101
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://www3.interscience.wiley.com/journal/119267376/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 635245 bytes
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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