Removal of nuclear contaminants and of non-specifically photosystem II-bound copper from photosystem II preparations
In conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was asso...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 1994 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/11101 |
| Acceso en línea: | http://hdl.handle.net/10261/11101 |
| Access Level: | acceso abierto |
| Palabra clave: | Photosystem II Nuclear contaminants Copper Histones |
| Sumario: | In conventional photosystem II preparation high amounts of Cu are found. After fractionation by centrifugation, Cu can be completely removed from photosystem II without affecting either its photosynthetic activity or the composition of its specific proteins. We could demonstrate that the Cu was associated with nuclear contaminants in the starch fraction. Among the contaminants, several histones were identified by specific antisera and by N-terminal sequencing. In order to obtain homogeneous BBY preparations of PSII a procedure is employed that involves a 10000 g centrifugation step and which eliminates non-specifically bound metals, nucleic acids and histones with the starch pellet. The resulting starch-free BBY (BBYs-), which is free of these nuclear contaminants, is an appropriate preparation for biophysical studies or for those of metal interactions with PSII. |
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