Development of an electrochemical sensor for coeliac disease serological markers
Coeliac disease (CD), a gluten-sensitive enteropathy, is an autoimmune disorder of the upper small intestine triggered from the gluten ingestion (cereal protein found in wheat, rye and barley) and affects 1% of the population around the globe. The ingestion of gluten, triggers the production of a se...
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| Tipo de recurso: | tesis doctoral |
| Estado: | Versión publicada |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universitat Rovira i virgili (URV) |
| Repositorio: | Repositori Institucional de la Universitat Rovira i Virgili |
| OAI Identifier: | oai:urv.cat:TDX:1138 |
| Acceso en línea: | https://hdl.handle.net/20.500.11797/TDX1138 http://hdl.handle.net/10803/96661 |
| Access Level: | acceso abierto |
| Palabra clave: | 62 - Enginyeria. Tecnologia 577 - Bioquímica. Biologia molecular. Biofísica 543 - Química analítica |
| Sumario: | Coeliac disease (CD), a gluten-sensitive enteropathy, is an autoimmune disorder of the upper small intestine triggered from the gluten ingestion (cereal protein found in wheat, rye and barley) and affects 1% of the population around the globe. The ingestion of gluten, triggers the production of a series of autoantibodies against gliadin (AGA) and tissue transglutaminase (anti-tTG) which can provoke inflammation and damage some parts of the intestine. The detection of those antibodies through serological testing represent a non-invasive, fast and reliable approach. The main objective of this Thesis is the development of a sensitive, rapid and cost-efficient real-sample-oriented immunosensor using thiol-self assembled monolayers on gold surfaces. Two strategies for the antigen immobilisation have been investigated: i) The use of monolayers of a carboxylic acid-ended bipodal alkanethiol, ii) The introduction of disulfide groups through three different moieties of the antigens: amine, carboxylic and hydroxyl. Both immunosensor approaches were optimized and used for the amperometric detection of CD serological markers from human serum samples. |
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