Transcriptomic analysis reveals the wound healing activity of Mussel Myticin C

18 pags., 6 figs., 2 tabs.

Detalles Bibliográficos
Autores: Rey-Campos, Magalí, Moreira, Rebeca, Romero Jódar, Alejandro, Medina-Gali, Regla María, Novoa, Beatriz, Gasset, M., Figueras Huerta, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/218886
Acceso en línea:http://hdl.handle.net/10261/218886
Access Level:acceso abierto
Palabra clave:Mytilus galloprovincialis
Myticin C
Hemocytes
Chemotaxis
Illumina
Wound healing
Tissue injury
Regeneration
id ES_76eae377ee9d2c12ae5facf58b35e014
oai_identifier_str oai:digital.csic.es:10261/218886
network_acronym_str ES
network_name_str España
repository_id_str
spelling Transcriptomic analysis reveals the wound healing activity of Mussel Myticin CRey-Campos, MagalíMoreira, RebecaRomero Jódar, AlejandroMedina-Gali, Regla MaríaNovoa, BeatrizGasset, M.Figueras Huerta, AntonioMytilus galloprovincialisMyticin CHemocytesChemotaxisIlluminaWound healingTissue injuryRegeneration18 pags., 6 figs., 2 tabs.Myticin C is the most studied antimicrobial peptide in the marine mussel Mytilus galloprovincialis. Although it is constitutively expressed in mussel hemocytes and displays antibacterial, antiviral, and chemotactic functions, recent work has suggested that this molecule is mainly activated after tissue injury. Therefore, the main objective of this work was to characterize the hemocytes’ transcriptomic response after a myticin C treatment, in order to understand the molecular changes induced by this cytokine-like molecule. The transcriptome analysis revealed the modulation of genes related to cellular movement, such as myosin, transgelin, and calponin-like proteins, in agreement with results of functional assays, where an implication of myticin C in the in vitro activation of hemocytes and migration was evidenced. This was also observed in vivo after a tissue injury, when hemocytes, with high concentrations of myticin C, migrated to the damaged area to heal the wound. All these properties allowed us to think about the biotechnological application of these molecules as wound healers. Human keratinocytes and larvae zebrafish models were used to confirm this hypothesis. Accelerated regeneration after a wound or tail fin amputation was observed after treatment with the myticin C peptide, supporting the chemotactic and healing activity of myticin C.This research was funded in parts by the Spanish AEI/EU-FEDER (RTI2018-095997-B-I00); Consellería de Economía, Emprego e Industria (GAIN), Xunta de Galicia (IN607B 2019/01); the EU-H2020 VIVALDI (678589); and the European Regional Development Fund (ERDF) Interreg V Spain—Portugal (OPE01833). M.R.-C. was supported by a Spanish AEI/EU-FEDER predoctoral contract (BES-2016-076302).Multidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Xunta de GaliciaEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/218886reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095997-B-I00info:eu-repo/grantAgreement/EC/H2020/678589http://dx.doi.org/10.3390/biom10010133Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2188862026-05-22T06:33:51Z
dc.title.none.fl_str_mv Transcriptomic analysis reveals the wound healing activity of Mussel Myticin C
title Transcriptomic analysis reveals the wound healing activity of Mussel Myticin C
spellingShingle Transcriptomic analysis reveals the wound healing activity of Mussel Myticin C
Rey-Campos, Magalí
Mytilus galloprovincialis
Myticin C
Hemocytes
Chemotaxis
Illumina
Wound healing
Tissue injury
Regeneration
title_short Transcriptomic analysis reveals the wound healing activity of Mussel Myticin C
title_full Transcriptomic analysis reveals the wound healing activity of Mussel Myticin C
title_fullStr Transcriptomic analysis reveals the wound healing activity of Mussel Myticin C
title_full_unstemmed Transcriptomic analysis reveals the wound healing activity of Mussel Myticin C
title_sort Transcriptomic analysis reveals the wound healing activity of Mussel Myticin C
dc.creator.none.fl_str_mv Rey-Campos, Magalí
Moreira, Rebeca
Romero Jódar, Alejandro
Medina-Gali, Regla María
Novoa, Beatriz
Gasset, M.
Figueras Huerta, Antonio
author Rey-Campos, Magalí
author_facet Rey-Campos, Magalí
Moreira, Rebeca
Romero Jódar, Alejandro
Medina-Gali, Regla María
Novoa, Beatriz
Gasset, M.
Figueras Huerta, Antonio
author_role author
author2 Moreira, Rebeca
Romero Jódar, Alejandro
Medina-Gali, Regla María
Novoa, Beatriz
Gasset, M.
Figueras Huerta, Antonio
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Xunta de Galicia
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Mytilus galloprovincialis
Myticin C
Hemocytes
Chemotaxis
Illumina
Wound healing
Tissue injury
Regeneration
topic Mytilus galloprovincialis
Myticin C
Hemocytes
Chemotaxis
Illumina
Wound healing
Tissue injury
Regeneration
description 18 pags., 6 figs., 2 tabs.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/218886
url http://hdl.handle.net/10261/218886
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095997-B-I00
info:eu-repo/grantAgreement/EC/H2020/678589
http://dx.doi.org/10.3390/biom10010133
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411084891324416
score 15,81155

Similares en LA Referencia