Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)
The inclusion of a medicinal plant leaf extract (MPLE) from sage (Salvia officinalis) and lemon verbena (Lippia citriodora), rich in verbascoside and triterpenic compounds like ursolic acid, was evaluated in gilthead seabream (Sparus aurata) fed a low fishmeal-based diet (48% crude protein, 17% crud...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/253585 |
| Acceso en línea: | http://hdl.handle.net/10261/253585 |
| Access Level: | acceso abierto |
| Palabra clave: | Cell proliferation Feed additives Gut health Innate immunity Lectin histochemistry Ursolic acid Verbascoside acid GALT |
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Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata) |
| title |
Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata) |
| spellingShingle |
Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata) Salomón, Ricardo Cell proliferation Feed additives Gut health Innate immunity Lectin histochemistry Ursolic acid Verbascoside acid GALT |
| title_short |
Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata) |
| title_full |
Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata) |
| title_fullStr |
Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata) |
| title_full_unstemmed |
Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata) |
| title_sort |
Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata) |
| dc.creator.none.fl_str_mv |
Salomón, Ricardo Reyes-López, Felipe E. Tort, Lluis Firmino, Joana P. Sarasquete, Carmen Ortiz-Delgado, Juan B. Quintela, José C. Pinilla-Rosas, José M. Vallejos-Vidal, Eva Gisbert, Enric |
| author |
Salomón, Ricardo |
| author_facet |
Salomón, Ricardo Reyes-López, Felipe E. Tort, Lluis Firmino, Joana P. Sarasquete, Carmen Ortiz-Delgado, Juan B. Quintela, José C. Pinilla-Rosas, José M. Vallejos-Vidal, Eva Gisbert, Enric |
| author_role |
author |
| author2 |
Reyes-López, Felipe E. Tort, Lluis Firmino, Joana P. Sarasquete, Carmen Ortiz-Delgado, Juan B. Quintela, José C. Pinilla-Rosas, José M. Vallejos-Vidal, Eva Gisbert, Enric |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Ministerio de Agricultura, Pesca y Alimentación y Medio Ambiente (España) European Commission European Research Council Fondo Nacional de Desarrollo Científico y Tecnológico (Chile) Agencia Nacional de Investigación y Desarrollo (Chile) Programa Nacional de Becas de Postgrado en el Exterior Don Carlos Antonio López (Paraguay) Generalitat de Catalunya Universidad de Santiago de Chile Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Cell proliferation Feed additives Gut health Innate immunity Lectin histochemistry Ursolic acid Verbascoside acid GALT |
| topic |
Cell proliferation Feed additives Gut health Innate immunity Lectin histochemistry Ursolic acid Verbascoside acid GALT |
| description |
The inclusion of a medicinal plant leaf extract (MPLE) from sage (Salvia officinalis) and lemon verbena (Lippia citriodora), rich in verbascoside and triterpenic compounds like ursolic acid, was evaluated in gilthead seabream (Sparus aurata) fed a low fishmeal-based diet (48% crude protein, 17% crude fat, 21.7 MJ kg-1, 7% fishmeal, 15% fish oil) for 92 days. In particular, the study focused on the effect of these phytogenic compounds on the gut condition by analyzing the transcriptomic profiling (microarray analysis) and histological structure of the intestinal mucosa, as well as the histochemical properties of mucins stored in goblet cells. A total number of 506 differentially expressed genes (285 up- and 221 down-regulated) were found when comparing the transcriptomic profiling of the intestine from fish fed the control and MPLE diets. The gut transcripteractome revealed an expression profile that favored biological mechanisms associated to the 1) immune system, particularly involving T cell activation and differentiation, 2) gut integrity (i.e., adherens and tight junctions) and cellular proliferation, and 3) cellular proteolytic pathways. The histological analysis showed that the MPLE dietary supplementation promoted an increase in the number of intestinal goblet cells and modified the composition of mucins’ glycoproteins stored in goblet cells, with an increase in the staining intensity of neutral mucins, as well as in mucins rich in carboxylated and weakly sulfated glycoconjugates, particularly those rich in sialic acid residues. The integration of transcriptomic and histological results showed that the evaluated MPLE from sage and lemon verbena is responsible for the maintenance of intestinal health, supporting gut homeostasis and increasing the integrity of the intestinal epithelium, which suggests that this phytogenic may be considered as a promising sustainable functional additive for aquafeeds. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2021 2021 2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/253585 |
| url |
http://hdl.handle.net/10261/253585 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/727315 http://dx.doi.org/10.3389/fimmu.2021.670279 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.publisher.none.fl_str_mv |
Frontiers Media |
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Frontiers Media |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869411038621859840 |
| spelling |
Medicinal Plant Leaf Extract From Sage and Lemon Verbena Promotes Intestinal Immunity and Barrier Function in Gilthead Seabream (Sparus aurata)Salomón, RicardoReyes-López, Felipe E.Tort, LluisFirmino, Joana P.Sarasquete, CarmenOrtiz-Delgado, Juan B.Quintela, José C.Pinilla-Rosas, José M.Vallejos-Vidal, EvaGisbert, EnricCell proliferationFeed additivesGut healthInnate immunityLectin histochemistryUrsolic acidVerbascoside acidGALTThe inclusion of a medicinal plant leaf extract (MPLE) from sage (Salvia officinalis) and lemon verbena (Lippia citriodora), rich in verbascoside and triterpenic compounds like ursolic acid, was evaluated in gilthead seabream (Sparus aurata) fed a low fishmeal-based diet (48% crude protein, 17% crude fat, 21.7 MJ kg-1, 7% fishmeal, 15% fish oil) for 92 days. In particular, the study focused on the effect of these phytogenic compounds on the gut condition by analyzing the transcriptomic profiling (microarray analysis) and histological structure of the intestinal mucosa, as well as the histochemical properties of mucins stored in goblet cells. A total number of 506 differentially expressed genes (285 up- and 221 down-regulated) were found when comparing the transcriptomic profiling of the intestine from fish fed the control and MPLE diets. The gut transcripteractome revealed an expression profile that favored biological mechanisms associated to the 1) immune system, particularly involving T cell activation and differentiation, 2) gut integrity (i.e., adherens and tight junctions) and cellular proliferation, and 3) cellular proteolytic pathways. The histological analysis showed that the MPLE dietary supplementation promoted an increase in the number of intestinal goblet cells and modified the composition of mucins’ glycoproteins stored in goblet cells, with an increase in the staining intensity of neutral mucins, as well as in mucins rich in carboxylated and weakly sulfated glycoconjugates, particularly those rich in sialic acid residues. The integration of transcriptomic and histological results showed that the evaluated MPLE from sage and lemon verbena is responsible for the maintenance of intestinal health, supporting gut homeostasis and increasing the integrity of the intestinal epithelium, which suggests that this phytogenic may be considered as a promising sustainable functional additive for aquafeeds.This work has been financially supported by the project “Nutritional strategies for the improvement of productive performance: the use of functional feeds and health diets in aquaculture (DIETAplus)”, funded by JACUMAR (Ministry of Agriculture, Fisheries and Environment of Spain, MAPAMA) and FEMP (EU), as well as by the ERC (European Research Council) in MedAID project (Mediterranean Aquaculture Integrated Development; Grant Agreement Nb. 727315). FR-L thanks the support of Fondecyt Regular grant (Nb. 1211841; ANID; Government of Chile). RS is supported by a PhD grant from the government of Paraguay (BECAL). JF have been subsidized by the Industrial PhD program of Generalitat de Catalunya and TECNOVIT-FARMFAES S.L. (Nb. 2017 DI 017). EV-V was financially granted with DICYT-USACH Postdoctoral fellowship (Nb. 022043IB). Collaboration between Ibero-American researchers has been done under the framework of the network LARVAplus “Strategies for the development and improvement of fish larvae production in Ibero-America” (117RT0521) funded by the Ibero-American Program of Science and Technology for Development (CYTED, Spain).Frontiers MediaMinisterio de Agricultura, Pesca y Alimentación y Medio Ambiente (España)European CommissionEuropean Research CouncilFondo Nacional de Desarrollo Científico y Tecnológico (Chile)Agencia Nacional de Investigación y Desarrollo (Chile)Programa Nacional de Becas de Postgrado en el Exterior Don Carlos Antonio López (Paraguay)Generalitat de CatalunyaUniversidad de Santiago de ChilePrograma Iberoamericano de Ciencia y Tecnología para el DesarrolloConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/253585reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/727315http://dx.doi.org/10.3389/fimmu.2021.670279Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2535852026-05-22T06:33:51Z |
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15,81155 |