Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of Suckling

Immune system development during gestation and suckling is significantly modulated by maternal environmental and dietary factors. Breastfeeding is widely recognized as the optimal source of nutrition for infant growth and immune maturation, and its composition can be modulated by the maternal diet....

Descripción completa

Detalles Bibliográficos
Autores: Sáez Fuertes, Laura, Kapravelou, Garyfallia, Grases Pintó, Blanca, Bernabeu, Manuel, Knipping, Karen, Garssen, Johan, Bourdet-Sicard, Raphaëlle, Castell, Margarida, Collado, María Carmen, Pérez-Cano, Francisco José, Rodríguez-Lagunas, María José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/362722
Acceso en línea:http://hdl.handle.net/10261/362722
Access Level:acceso abierto
Palabra clave:Breastfeeding
Bifidobacterirum breve M-16V
scGOS/lcFOS
Immune system
immune system
id ES_75b68e61ba1e33be65d3652d91bff7fa
oai_identifier_str oai:digital.csic.es:10261/362722
network_acronym_str ES
network_name_str España
repository_id_str
spelling Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of SucklingSáez Fuertes, LauraKapravelou, GaryfalliaGrases Pintó, BlancaBernabeu, ManuelKnipping, KarenGarssen, JohanBourdet-Sicard, RaphaëlleCastell, MargaridaCollado, María CarmenPérez-Cano, Francisco JoséRodríguez-Lagunas, María JoséBreastfeedingBifidobacterirum breve M-16VscGOS/lcFOSImmune systemimmune systemImmune system development during gestation and suckling is significantly modulated by maternal environmental and dietary factors. Breastfeeding is widely recognized as the optimal source of nutrition for infant growth and immune maturation, and its composition can be modulated by the maternal diet. In the present work, we investigated whether oral supplementation with Bifidobacterium breve M-16V and short-chain galacto-oligosaccharide (scGOS) and long-chain fructo-oligosaccharide (lcFOS) to rat dams during gestation and lactation has an impact on the immune system and microbiota composition of the offspring at day 21 of life. On that day, blood, adipose tissue, small intestine (SI), mesenteric lymph nodes (MLN), salivary gland (SG), cecum, and spleen were collected. Synbiotic supplementation did not affect the overall body or organ growth of the pups. The gene expression of Tlr9, Muc2, IgA, and Blimp1 were upregulated in the SI, and the increase in IgA gene expression was further confirmed at the protein level in the gut wash. Synbiotic supplementation also positively impacted the microbiota composition in both the small and large intestines, resulting in higher proportions of Bifidobacterium genus, among others. In addition, there was an increase in butanoic, isobutanoic, and acetic acid concentrations in the cecum but a reduction in the small intestine. At the systemic level, synbiotic supplementation resulted in higher levels of immunoglobulin IgG2c in plasma, SG, and MLN, but it did not modify the main lymphocyte subsets in the spleen and MLN. Overall, synbiotic maternal supplementation is able to positively influence the immune system development and microbiota of the suckling offspring, particularly at the gastrointestinal levelThe research described in this paper was supported by LaMarató-TV3 (DIM-2-ELI, ref. 2018-27/30-31). L.S-F. holds a fellowship from the Generalitat de Catalunya, FI-SDUR (2023 FISDU 00035)With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2021-001189-S).Peer reviewedMultidisciplinary Digital Publishing InstituteFundació La Marató de TV3Generalitat de CatalunyaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2024202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/362722reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Español#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001189-SThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/nu16121890https://doi.org/10.3390/nu16121890Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3627222026-05-22T06:33:51Z
dc.title.none.fl_str_mv Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of Suckling
title Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of Suckling
spellingShingle Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of Suckling
Sáez Fuertes, Laura
Breastfeeding
Bifidobacterirum breve M-16V
scGOS/lcFOS
Immune system
immune system
title_short Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of Suckling
title_full Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of Suckling
title_fullStr Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of Suckling
title_full_unstemmed Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of Suckling
title_sort Maternal Synbiotic Supplementation with B. breve M-16V and scGOS/lcFOS Shape Offspring Immune Development and Gut Microbiota at the End of Suckling
dc.creator.none.fl_str_mv Sáez Fuertes, Laura
Kapravelou, Garyfallia
Grases Pintó, Blanca
Bernabeu, Manuel
Knipping, Karen
Garssen, Johan
Bourdet-Sicard, Raphaëlle
Castell, Margarida
Collado, María Carmen
Pérez-Cano, Francisco José
Rodríguez-Lagunas, María José
author Sáez Fuertes, Laura
author_facet Sáez Fuertes, Laura
Kapravelou, Garyfallia
Grases Pintó, Blanca
Bernabeu, Manuel
Knipping, Karen
Garssen, Johan
Bourdet-Sicard, Raphaëlle
Castell, Margarida
Collado, María Carmen
Pérez-Cano, Francisco José
Rodríguez-Lagunas, María José
author_role author
author2 Kapravelou, Garyfallia
Grases Pintó, Blanca
Bernabeu, Manuel
Knipping, Karen
Garssen, Johan
Bourdet-Sicard, Raphaëlle
Castell, Margarida
Collado, María Carmen
Pérez-Cano, Francisco José
Rodríguez-Lagunas, María José
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundació La Marató de TV3
Generalitat de Catalunya
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Breastfeeding
Bifidobacterirum breve M-16V
scGOS/lcFOS
Immune system
immune system
topic Breastfeeding
Bifidobacterirum breve M-16V
scGOS/lcFOS
Immune system
immune system
description Immune system development during gestation and suckling is significantly modulated by maternal environmental and dietary factors. Breastfeeding is widely recognized as the optimal source of nutrition for infant growth and immune maturation, and its composition can be modulated by the maternal diet. In the present work, we investigated whether oral supplementation with Bifidobacterium breve M-16V and short-chain galacto-oligosaccharide (scGOS) and long-chain fructo-oligosaccharide (lcFOS) to rat dams during gestation and lactation has an impact on the immune system and microbiota composition of the offspring at day 21 of life. On that day, blood, adipose tissue, small intestine (SI), mesenteric lymph nodes (MLN), salivary gland (SG), cecum, and spleen were collected. Synbiotic supplementation did not affect the overall body or organ growth of the pups. The gene expression of Tlr9, Muc2, IgA, and Blimp1 were upregulated in the SI, and the increase in IgA gene expression was further confirmed at the protein level in the gut wash. Synbiotic supplementation also positively impacted the microbiota composition in both the small and large intestines, resulting in higher proportions of Bifidobacterium genus, among others. In addition, there was an increase in butanoic, isobutanoic, and acetic acid concentrations in the cecum but a reduction in the small intestine. At the systemic level, synbiotic supplementation resulted in higher levels of immunoglobulin IgG2c in plasma, SG, and MLN, but it did not modify the main lymphocyte subsets in the spleen and MLN. Overall, synbiotic maternal supplementation is able to positively influence the immune system development and microbiota of the suckling offspring, particularly at the gastrointestinal level
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/362722
url http://hdl.handle.net/10261/362722
dc.language.none.fl_str_mv Español
language_invalid_str_mv Español
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/CEX2021-001189-S
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.3390/nu16121890
https://doi.org/10.3390/nu16121890
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410999825596416
score 15,811543

Similares en LA Referencia