Functional Homology among Human and Fission Yeast Cdc14 Phosphatases

[EN] Budding and fission yeast Cdc14 homologues, a conserved family of serine-threonine phosphatases, play a role in the inactivation of mitotic cyclin-dependent kinases (CDKs) by molecularly distinct mechanisms. Saccharomyces cerevisiae Cdc14 protein phosphatase inactivates CDKs by promoting mitoti...

Descripción completa

Detalles Bibliográficos
Autores: Vázquez-Novelle, M. Dolores, Esteban, Verónica, Bueno Núñez, Andrés Avelino, Sacristán Martín, María Paz
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2005
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/157107
Acceso en línea:http://hdl.handle.net/10366/157107
Access Level:acceso abierto
Palabra clave:Cell Cycle
Dephosphorylation
Cdc14 phosphatase
2302 Bioquímica
2415 Biología Molecular
2407 Biología Celular
2409 Genética
id ES_754a3e8d8dcd5f70ac4fb9168571aeec
oai_identifier_str oai:gredos.usal.es:10366/157107
network_acronym_str ES
network_name_str España
repository_id_str
spelling Functional Homology among Human and Fission Yeast Cdc14 PhosphatasesVázquez-Novelle, M. DoloresEsteban, VerónicaBueno Núñez, Andrés AvelinoSacristán Martín, María PazCell CycleDephosphorylationCdc14 phosphatase2302 Bioquímica2415 Biología Molecular2407 Biología Celular2409 Genética[EN] Budding and fission yeast Cdc14 homologues, a conserved family of serine-threonine phosphatases, play a role in the inactivation of mitotic cyclin-dependent kinases (CDKs) by molecularly distinct mechanisms. Saccharomyces cerevisiae Cdc14 protein phosphatase inactivates CDKs by promoting mitotic cyclin degradation and the accumulation of a CDK inhibitor to allow budding yeast cells to exit from mitosis. Schizosaccharomyces pombe Flp1 phosphatase down-regulates CDK/cyclin activity, controlling the degradation of the Cdc25 tyrosine phosphatase for fission yeast cells to undergo cytokinesis. In the present work, we show that human Cdc14 homologues (hCdc14A and hCdc14B) rescued flp1-deficient fission yeast strains, indicating functional homology. We also show that hCdc14A and B interacted in vivo with S. pombe Cdc25 and that hCdc14A dephosphorylated this mitotic inducer both in vitro and in vivo. Our results support a Cdc14 conserved inhibitory mechanism acting on S. pombe Cdc25 protein and suggest that human cells may regulate Cdc25 in a similar manner to inactivate Cdk1-mitotic cyclin complexes.ASBMB202420242005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10366/157107reponame:GREDOS. Repositorio Institucional de la Universidad de Salamancainstname:Universidad de Salamanca (USAL)InglésCC0 1.0 Universalhttp://creativecommons.org/publicdomain/zero/1.0/info:eu-repo/semantics/openAccessoai:gredos.usal.es:10366/1571072026-06-07T06:28:51Z
dc.title.none.fl_str_mv Functional Homology among Human and Fission Yeast Cdc14 Phosphatases
title Functional Homology among Human and Fission Yeast Cdc14 Phosphatases
spellingShingle Functional Homology among Human and Fission Yeast Cdc14 Phosphatases
Vázquez-Novelle, M. Dolores
Cell Cycle
Dephosphorylation
Cdc14 phosphatase
2302 Bioquímica
2415 Biología Molecular
2407 Biología Celular
2409 Genética
title_short Functional Homology among Human and Fission Yeast Cdc14 Phosphatases
title_full Functional Homology among Human and Fission Yeast Cdc14 Phosphatases
title_fullStr Functional Homology among Human and Fission Yeast Cdc14 Phosphatases
title_full_unstemmed Functional Homology among Human and Fission Yeast Cdc14 Phosphatases
title_sort Functional Homology among Human and Fission Yeast Cdc14 Phosphatases
dc.creator.none.fl_str_mv Vázquez-Novelle, M. Dolores
Esteban, Verónica
Bueno Núñez, Andrés Avelino
Sacristán Martín, María Paz
author Vázquez-Novelle, M. Dolores
author_facet Vázquez-Novelle, M. Dolores
Esteban, Verónica
Bueno Núñez, Andrés Avelino
Sacristán Martín, María Paz
author_role author
author2 Esteban, Verónica
Bueno Núñez, Andrés Avelino
Sacristán Martín, María Paz
author2_role author
author
author
dc.subject.none.fl_str_mv Cell Cycle
Dephosphorylation
Cdc14 phosphatase
2302 Bioquímica
2415 Biología Molecular
2407 Biología Celular
2409 Genética
topic Cell Cycle
Dephosphorylation
Cdc14 phosphatase
2302 Bioquímica
2415 Biología Molecular
2407 Biología Celular
2409 Genética
description [EN] Budding and fission yeast Cdc14 homologues, a conserved family of serine-threonine phosphatases, play a role in the inactivation of mitotic cyclin-dependent kinases (CDKs) by molecularly distinct mechanisms. Saccharomyces cerevisiae Cdc14 protein phosphatase inactivates CDKs by promoting mitotic cyclin degradation and the accumulation of a CDK inhibitor to allow budding yeast cells to exit from mitosis. Schizosaccharomyces pombe Flp1 phosphatase down-regulates CDK/cyclin activity, controlling the degradation of the Cdc25 tyrosine phosphatase for fission yeast cells to undergo cytokinesis. In the present work, we show that human Cdc14 homologues (hCdc14A and hCdc14B) rescued flp1-deficient fission yeast strains, indicating functional homology. We also show that hCdc14A and B interacted in vivo with S. pombe Cdc25 and that hCdc14A dephosphorylated this mitotic inducer both in vitro and in vivo. Our results support a Cdc14 conserved inhibitory mechanism acting on S. pombe Cdc25 protein and suggest that human cells may regulate Cdc25 in a similar manner to inactivate Cdk1-mitotic cyclin complexes.
publishDate 2005
dc.date.none.fl_str_mv 2005
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10366/157107
url http://hdl.handle.net/10366/157107
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv CC0 1.0 Universal
http://creativecommons.org/publicdomain/zero/1.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv CC0 1.0 Universal
http://creativecommons.org/publicdomain/zero/1.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ASBMB
publisher.none.fl_str_mv ASBMB
dc.source.none.fl_str_mv reponame:GREDOS. Repositorio Institucional de la Universidad de Salamanca
instname:Universidad de Salamanca (USAL)
instname_str Universidad de Salamanca (USAL)
reponame_str GREDOS. Repositorio Institucional de la Universidad de Salamanca
collection GREDOS. Repositorio Institucional de la Universidad de Salamanca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410970009337856
score 15,300719

Similares en LA Referencia